We narrowed to 821 results for: LAS

...been cloned into plasmids. Because their sequences are known and encoded on a plasmid, they can be easily...are identical for antibodies within certain classification groups called isotypes.  Why does isotype matter...recombinant antibodies. Recombinant antibodies are plasmid-based and are created synthetically or derived ...regions of a specific antibody are cloned into a plasmid backbone containing all constant regions, just ...

...transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral plasmid containing the cargo...the kidney—either endothelial, epithelial, or fibroblast. However, Frank Graham showed HEKs display many...T-antigen. This allows them to replicate viral plasmids with the SV40 origin of replication. To generate...cell lines are susceptible to contamination by mycoplasma and bacteria, but HEKs are also particularly ...

...This construct will serve as the repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins...proteins. The repair template and Cas9-sgRNA plasmid is then injected into the germline of young adult ....115.178335. [Epub ahead of print] PubMed. Find plasmids from this publication at Addgene. Other strategies...10.1038/nmeth.2641. Epub 2013 Sep 1. PubMed. Find plasmids from this publication at Addgene. Scalable and...genetics.114.170423. Epub 2014 Sep 23. PubMed. Find plasmids from this publication at Addgene. Additional...

...), 339-350 (2015). PubMed. Find the Plasmids @Addgene: Plasmids for CRISPRi are described here: http...constructs to be introduced simultaneously in a single plasmid. The functionality of the RNA scaffolds follows...http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan et al. Cell  (2015) can be found here...https://www.addgene.org/browse/article/9593/ Plasmids described in Qi et al. Cell  (2013) can be found...

...Looking for Optogenetics Plasmids? Plasmids for Optogenetics Research (Addgene) Plasmids from Ed Boyden's lab...conditions. Addgene: You've contributed various plasmids to Addgene over the years, many of them very popular... lab Sign up to receive email alerts when new plasmids from the Boyden lab become available. Visit Ed...

...to elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources on...studies and is not used as much for the standard plasmid prep. Diphenylamine method Another absorbance based...reader if no other instruments are available. Some last reminders about quantifying DNA In choosing your...Addgene.org Visit the Molecular Biology Reference Find plasmids for your research Browse protocols for molecular...

...going on there!).  Find the Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6 construct is undergoing QC and... has been inundated with requests to share our plasmids with other researchers. We have been excited to...Gordon) have been quite busy shipping and sending plasmids to all who request them -- in locations all over...Facebook. Find more COVID-19 and coronavirus related plasmids and resources at Addgene* Many thanks to our...people, not just scientists! *If you are requesting plasmids for coronavirus or COVID-19 research, please email...

...unscalable.  Melissa Chiasson, a graduate student in Douglas Fowler's lab at the University of Washington sought...aspect of life.” Unger began with the a bacterial periplasmic binding protein, one similar to the protein which.... Unger redesigned the binding pocket of the periplasmic binding protein so that it would recognize serotonin... one population responds to a second population. Last, Bernach will simultaneously monitor cell cycle ...Addgene.org Find fluorescent protein biosensors Browse Plasmids from the Tsien lab Browse the Michael Davidson...

...cleave proteins. Executioner caspases are activated last in the cascade and are responsible for the degradation...staining, a popular method to observe apoptotic cells. Lastly, the Shu Lab demonstrated that the FlipGFP-based...these diseases and disorders. You can find the plasmids for FlipGFP and FlipCherry at Addgene. References...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...protein fusions Browse the fluorescent proteins plasmid kits ...

...-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology Plasmids ...are single cell, eukaryotic organisms formally classified as fungi. They reproduce by either fission or..., yielding higher production power from a given flask volume. What’s even better? K. phaffii is highly...the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the...