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We narrowed to 814 results for: LAS

Showing: 441 - 450 of 814 results

  1. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...to elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources on...studies and is not used as much for the standard plasmid prep. Diphenylamine method Another absorbance based...reader if no other instruments are available. Some last reminders about quantifying DNA In choosing your...Addgene.org Visit the Molecular Biology Reference Find plasmids for your research Browse protocols for molecular...

  2. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...going on there!).  Find the Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6 construct is undergoing QC and... has been inundated with requests to share our plasmids with other researchers. We have been excited to...Gordon) have been quite busy shipping and sending plasmids to all who request them -- in locations all over...Facebook. Find more COVID-19 and coronavirus related plasmids and resources at Addgene* Many thanks to our...people, not just scientists! *If you are requesting plasmids for coronavirus or COVID-19 research, please email...

  3. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...cleave proteins. Executioner caspases are activated last in the cascade and are responsible for the degradation...staining, a popular method to observe apoptotic cells. Lastly, the Shu Lab demonstrated that the FlipGFP-based...these diseases and disorders. You can find the plasmids for FlipGFP and FlipCherry at Addgene. References...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...protein fusions Browse the fluorescent proteins plasmid kits ...

  4. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    Published
    June 4, 2019, 1:02 p.m.
    ...unscalable.  Melissa Chiasson, a graduate student in Douglas Fowler's lab at the University of Washington sought...aspect of life.” Unger began with the a bacterial periplasmic binding protein, one similar to the protein which.... Unger redesigned the binding pocket of the periplasmic binding protein so that it would recognize serotonin... one population responds to a second population. Last, Bernach will simultaneously monitor cell cycle ...Addgene.org Find fluorescent protein biosensors Browse Plasmids from the Tsien lab Browse the Michael Davidson...

  5. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...microbiology class my sophomore year and it was the first time that I was in a lab class where we had ...has been critical to the Broad’s success over the last 11 years and was certainly critical to drawing me...like... when it looks good, that’s a high that can last for several days.” Working with CRISPR Recently...and talk to the people who actually have these plasmids, figure out what they do and what they might be...

  6. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...t require an excitation light source (usually a laser in fluorescence assays), which means luminescence...Engineered   677 61 AkaLumine ATP No Common uses for plasmids expressing luciferase Luciferase reporter assays... partnered with Promega to offer their Promega Plasmid Collection, including tools using NanoLuc®, NanoBiT...collection page Promega Plasmid Collection Fluorescent Proteins 101 ebook ... plasmids 101...

  7. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ... towards cold, we decided to focus on a special class of enzymes expressed by psychrophilic (cold-loving...transformed mesophilic, for example E. coli, carrying a plasmid expressing a protein of interest). Mesophilic microbes...granules. We overcame this problem by engineering a plasmid which contained cold tolerant chaperones (the Cpn...pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a ...biology blog posts Resources on Addgene.org Find plasmids for your synthetic biology experiment Read our...

  8. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...websites, we built our new search engine atop Elasticsearch, a powerful open-source search engine that can...experience experts, our software team tailored Elasticsearch to support the different usage patterns the ...and depositors — each with subcategories such as plasmids and viral preps for catalog, blog posts and protocols... and category. For example, a search scoped to plasmids shows filters for expression and vector type, ...related items (for example, a kit that contains a plasmid with the catalog ID). Depositor suggestions provide...

  9. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...EsCas13d. Find the Yan et al. plasmids here Find the Konermann et al. Plasmids here How do Cas13d enzymes...S0092-8674(18)30207-1. PubMed PMID: 29551272 Plasmids from this paper are available at Addgene. Yan...S1097-2765(18)30173-4. PubMed PMID: 29551514 Plasmids from this paper are available at Addgene. Learn... Resources on Addgene.org: Find RNA Knockdown Plasmids Check out our CRISPR Guide Read our CRISPR History...

  10. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ... If there’s a plasmid or AAV tool that you can’t find, you can always suggest a plasmid to us, request...want to get into the field. Find Optogenetics Plasmids for Use in Neuroscience Research Neuroscience ...400 who signed up for our newsletter and a free plasmid) were very excited about our new viral services...Service Find Retrograde AAV Browse Optogenetics Plasmids ...
Showing: 441 - 450 of 814 results