We narrowed to 814 results for: LAS
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TypeBlog PostPublishedFeb. 19, 2019, 1:36 p.m....that Addgene provides is an open repository of plasmids and other necessary information that’s required...the collection, storage, and distribution of the plasmids and their ability to identify and fulfill a need... helping the scientific community manage those plasmids so scientists aren’t bogged down with distributing...continuing to look at providing other things besides plasmids to the scientific community through Addgene’s ...Information Systems Audit and Control Association. Lastly, Carl also serves on the Board of Advisors and ...
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Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m....tools for molecular and cell biologists over the last two decades, but their use can be limited by a few...: 1262. PMID: 23232392 PMCID: PMC3535334 Find plasmids from this paper at Addgene. Takai, Akira, et ...4352-6. PMID: 25831507. PMCID: PMC4394297 Find plasmids from this paper at Addgene. Suzuki, Kazushi, ... 13718. PMID: 27966527 PMCID: PMC5171807 Find plasmids from this paper at Addgene. Additional Resources...All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein ... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....deposits of plasmids that have been described in a preprint. In fact, over 1,500 plasmids in our catalog...Depositing plasmids that were used in a preprint provides all the same benefits as sharing those plasmids alongside...can include the Addgene ID for their deposited plasmids in the final journal publication or an updated... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m....isotype classes, it is possible to get secondary antibodies generated against isotype subclasses, such ...from a specific species, such as the rabbit IgG class of antibodies. They do so by targeting the Fc region...secondary antibodies which recognize specific isotype subclasses of primary antibodies can increase the possible...Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....their signal strength, and how long their signals last all impact transduction of the message. To study...ERK KTR and red fluorescence localizes to the cytoplasm. When MEK1 and ERK are inactive, the ERK KTR isn...pathway (Raf1, EGRF), the KTR localized to the cytoplasm. This all-optical psMEK1 + ERK KTR-mRuby2 system...photoswitchable kinases in my experiments? You can find the plasmids from Zhou et al here. In addition, it could be...GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable fluorescent proteins in ... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....CytERM, to direct the fluorescent protein to the Endoplasmic Reticulum (ER). If homodimerization occurs (most...typical structures known as Organized Smooth Endoplasmic Reticulum (OSER) whorls are visible. These whorls...Chudakov et al, 2007). We have developed a variety of plasmids that can be used in the OSER assay including CytERM-mKOkappa...documenting it in some other way. Besides the data, new plasmids and/or cells or organisms that are engineered ...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog PostPublishedJune 8, 2017, 2:30 p.m....between fluorescent proteins in the same spectral class (Cranfill et al, 2016). This non-linear dependence...excitation power (Shaner et al, 2008). In confocal laser scanning microscopy, fluorescent proteins are excited...localized fusion proteins and do not require dedicated plasmids or constructs. It should be noted that the photostability...several fluorescent proteins of the same spectral class on the microscopy system and in the cell type (or...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....24360272. PubMed Central PMCID: PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui...25713381. PubMed Central PMCID: PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui...27088723. PubMed Central PMCID: PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira...26344044. PubMed Central PMCID: PMC4666719. Find plasmids from this publication at Addgene. Additional ...Resources on Asddgene.org Find Fluorescent Protein Plasmids Find Fluorescent Protein Kits Find Empty Backbones... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog PostPublishedApril 12, 2016, 2:30 p.m....pool of lentiviral plasmids. Multiple rounds of digestion and ligation result in plasmid libraries containing...and ligation steps to build barcoded lentiviral plasmids containing one or more gRNAs. As each ligation...containing combinations of different gRNAs in each plasmid. Each gRNA combination can be tracked and quantitatively...Background with Our CRISPR Guide Pages Find CRISPR Plasmids Find More Tools for Combinatorial Engineering ... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog PostPublishedJune 26, 2018, 1:33 p.m....working with the Rare Genomics Institute to provide plasmid grants to researchers working on rare diseases ...Richard Gronostajski who each are being awarded free plasmids form Addgene to accelerate their research. Read... on to learn how these researchers plan to use plasmids from Addgene to advance their research on diseases...Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG patient-derived immune cell...Nfix-negative neural stem cells. The lab will use plasmids from Addgene to construct NFIX expression vectors...