...between fluorescent proteins in the same spectral class (Cranfill et al, 2016). This non-linear dependence...excitation power (Shaner et al, 2008). In confocal laser scanning microscopy, fluorescent proteins are excited...localized fusion proteins and do not require dedicated plasmids or constructs. It should be noted that the photostability...several fluorescent proteins of the same spectral class on the microscopy system and in the cell type (or...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

...deposits of plasmids that have been described in a preprint. In fact, over 1,500 plasmids in our catalog...Depositing plasmids that were used in a preprint provides all the same benefits as sharing those plasmids alongside...can include the Addgene ID for their deposited plasmids in the final journal publication or an updated...

...pool of lentiviral plasmids. Multiple rounds of digestion and ligation result in plasmid libraries containing...and ligation steps to build barcoded lentiviral plasmids containing one or more gRNAs. As each ligation...containing combinations of different gRNAs in each plasmid. Each gRNA combination can be tracked and quantitatively...Background with Our CRISPR Guide Pages Find CRISPR Plasmids Find More Tools for Combinatorial Engineering ...

...working with the Rare Genomics Institute to provide plasmid grants to researchers working on rare diseases ...Richard Gronostajski who each are being awarded free plasmids form Addgene to accelerate their research. Read... on to learn how these researchers plan to use plasmids from Addgene to advance their research on diseases...Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG patient-derived immune cell...Nfix-negative neural stem cells. The lab will use plasmids from Addgene to construct NFIX expression vectors...

...infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and viral transduction...types they tested. Interested in using Nanoblades? Plasmids are available from Addgene. Genome editing primary...to DNA transfection of gRNA and Cas9 expression plasmids. These results are in line with previous research...sometimes requires intracellular injections into the cytoplasm or pronucleus of the zygote which is both technically...out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral production ...

...in research can fall under one of two broad classifications: inbred or outbred. Inbred strains share a ...embryonic stem cells that are then injected into a blastocyst. There are several types of genetically engineered...Modified embryonic stem cells are injected into a blastocyst which is then implanted into a female animal ...embryonic stem cells, which are then injected into a blastocyst. Nucleases can also be used to engineer knockout...create mosaic animals where not all cells in the blastocyst were successfully edited by the injection of ...

...you the laser configuration. In general, if two fluorescent markers are read by the same laser, they will... markers you intend to use and your cytometer’s laser configuration. Your flow core or an online manual...reads out the fluorophores FITC and PE on the same laser, you will need to compensate between the two of ...your flow cytometer reads them out on different lasers, you should not need to compensate between them...

...JEDI, and many more. They can be targeted to the plasma membrane to measure membrane potential and can ...containing GEVIs. Chemigenetic sensors are better classified by their fluorescent reporter and the mechanism...fused to a single fluorescent protein (FP). This class of GEVI was the earliest developed voltage sensor...these sensors contained a FP that localized to the plasma membrane due to the addition of several targeting...lipophilic compound that distributes itself within the plasma membrane as well. Another flavor of these tools...

...transcription from the accessory plasmid (AP) encoding phage gene III. The accessory plasmid library contained all...PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the...doi: 10.1038/nature26155 PubMed PMID: 29512652 Plasmids from this paper are available at Addgene.  Additional...Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn about CRISPR History...

...antibiotic selectable markers by the destination plasmid. Antibiotic selection allowed for high throughput...accurate assemblies was achieved by the sequencing of plasmids isolated from blue or white colonies. Sequencing...occasional uncut or cut/re-ligated pGGA destination plasmids. A final validation of the 5-, 12- or 24-fragment...'s work on ligase efficiency Learn about other plasmid cloning techniques Resources on Addgene.org Find... Find basic molecular biology protocols Find plasmid cloning protocols Check out our molecular biology...