...Fluorescent tools for mammalian cells Nanobody purification CRISPRi and CRISPRa system for hPSCs The CRISPR...activity with a fluorescent protein--no affinity purification required! The team used other molecular techniques...compartments in mammalian cells By Angela Abitua Genetically encoded fluorescent proteins are often used to...collaborators have an ongoing effort to improve genetically encoded fluorescent markers for labeling specific...authors generated and described several improved genetically encoded fluorescent markers tagged with mTurquoise2...beads. Workflow of nanobody expression and purification. Iwaki et al., Protein Expr Purif, 2020. ...dCas9 and the gRNA. This expression can vary significantly depending on transgene design and delivery. ...

...be part of the answer. Problems with written publications Online tools like ResearchGate, GoogleScholar...from the science of others. Improving science communication through video     Video abstracts about research... to be that video is the future in terms of communicating ideas. In 2012, for example, 3 billion hours...video usurps the written word as the means for communicating research. Short videos of 3 to 5 minutes provide... researchers to leverage the video medium to communicate their research more effectively.” How to share...concentration of citations, 1900–2007." Journal of the American Society for Information Science and Technology...study." Journal of Librarianship and Scholarly Communication 2.2 (2014): 2. Additional Resources on the Addgene...

... line from a reputable distributor such as the American Type Culture Collection and freeze as many early...contamination. For those labs that prefer a more economical mode of detection, Uphoff et. al. outline a do... freshly collected lentivirus for downstream applications. While this ensures that the highest titer virus...virus. To circumvent this problem, transductions typically employ the positively charged polycation, polybrene...establishes the best conditions for specific applications, stick with them! Changes in such variables ...Ward T.H., Margison G.P. “Mycoplasma infection significantly alters microarray gene expression profiles.”...lentiviral and retroviral vectors.” Human Gene Therapy Clinical Development 25.3 (2014). 175-85. Pubmed PMID: ...

...Within that research group, you will also typically receive technical mentorship from a ‘bench mentor’. In ...receive after you’ve found your mentor, though. Typically, you will identify a ‘scientific mentor’: someone...scientific project. Your scientific mentor is typically a professor who operates a research group. Within...to do? Walk through the protocol mentally (or physically where relevant) and identify any gaps or questions...bring them to your mentor. Pro tip! Consider physically walking through the protocol, by going to each...something. It can be intimidating to ask for clarification at first, but in the end everyone wins. Your...experiments. Unlike in lab courses, where everything is magically made up by helpful TAs or assistants, and sitting...

... the bacteria were injected, limiting their applicability. They also did not scatter ultrasound nonlinearly...contrast in E. coli. We deleted an unannotated hypothetical protein, improving expression, and named this...promoter pBAD and a low-copy number origin of replication (p15A) worked best under a wide range of inducer...signal inside the tumors was overlaid with the anatomical B-mode images in greyscale.   ARG expression...of the mARG plamsids into HEK293T cells using a chemical transfection reagent (e.g. PEI-MAX). Simply cotransfect...-specific ultrasound signal is in hot and the anatomical B-mode images are in greyscale. (scalebar = 2mm...authors: Marjorie Buss is a PhD student studying chemical engineering. Mengtong "Tom" Duan is a PhD student...

...several ways. Below is a table listing some of critical differences (adapted from Zhou and Melton, 2008...this process. Moreover, it is much easier to genetically modify cells during the reprogramming process...This opens up a wide range of possibilities in clinical situations. In cases where the objective is to...Kushi) Mukherjee is the Editor of Trends in Pharmacological Sciences, a Cell Press reviews journal. She...joined Cell Press to pursue a career in science communication and publishing after completing her postdoctoral...from Massachusetts General Hospital and Harvard Medical School. Connect with her on LinkedIn @ https://...PMCID: PMC2854424. 23. Burridge, P.W., et al., Chemically defined generation of human cardiomyocytes. Nat...

...variety of species, cell types, and research applications. Labeling neurons with Brainbow The Lichtman...mOrange2, EGFP, and mKate2 (red) to solve the problematically low fluorescent intensity of previous versions... Brainbow 3.1 and 3.2 (described in the same publication) are unique because by default, they are not ...level of these XFP is not strong enough for all applications. To resolve this, similar systems have been ...developed a version of Tetbow that incorporates chemical tags instead of XFP to optimize it for tissue ...architecture of a fluorescent neuron is visible. Chemical tags are more resistant to tissue clearing methods...neuronal circuits with fluorescent proteins and chemical tags." eLife 7 (2018): e40350. PubMed PMID: 30454553...

...insights into the complicated physical organization of the brain and other biological systems. Interested...deletions are defined using 2 loxP variants (only identical loxP sites can mediate recombination.) The deletions...of farnesylated derivatives enables labeling of delicate axonal and dendritic processes not previously ...Between Brainbow Systems   Brainbow variants and applications beyond the mouse brain In addition to improving...temporal control without the need for germline modification, and enables Brainbow to be used in a variety...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Snippert HJ, van der...

...content of this article. Biosensors (‘biological sensors’) are biological tools that monitor a process or detect...a reporter signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent..., and you should always check the associated publication for the specifics of your chosen plasmid. Types...Experiments Experiment  Detection Method Notes Quantification of a process Plate-based assays (plate reader...components of individual cells Separation/identification of fluorescently-labeled components Fluorescence-activated... Jessica Welch...

...attended both online and on site courses with the American Society for Microbiology (ASM) Scientific Writing...of any scientist’s career. We use writing to communicate our findings and share them with the world. If...simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts and...field of microbiology with multiple high-profile publications. My classmates consisted of graduate students...sessions and reinforced our newfound knowledge with practical understanding of how to apply it. The personal...well as being critiqued added an extra level of application not present in the online class.   Four ways ...paper will be generated. After you complete a publication quality figure, produce the figure, its legend...