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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 772 results for: INA

Showing: 461 - 470 of 772 results

  1. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...to have a check-in with my boss Jennifer. I had finally found my footing with the company. I’d identified... Bench series about PPE! We made this with a combination of in-lab filming and remote filming. Learn: ...ensure smooth filming in the future, but we were finally refining the process down to a place I could be...

  2. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...Created with BioRender.com. In recent years, deaminase-mediated base editing (cytosine base editor or...FJ, Fan C, Landry MP (2019) DNA nanostructures coordinate gene silencing in mature plants. Proc Natl Acad...

  3. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...career scientists are those in the Young Scientist Seminar Series. Watch how these speakers talk without looking...their level of explanation in presenting data. Finally, let me take this opportunity to recommend all ...your slides for typos and clarity in a big room.  Finally, don’t pass up opportunities to show cool visuals...

  4. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...individuals. Antiretroviral therapy (ART) can virtually eliminate plasma HIV-1, improving life expectancy and quality... designed multiple gRNAs to target the 5’ long terminal repeat (LTR) of the HIV-1 genome, which acts as... Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination of HIV-1 Genomes from Human T-lymphoid Cells ...

  5. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers. Although the lab used this...thought to be reversible once the effector is inactivated/removed from the system. In contrast, epigenetic...

  6. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...While off-target effects are nearly impossible to eliminate, it’s essential to understand them in order to...repair to allow for detection, not Homologous Recombination or Alternative End Joining, two alternative ...different gRNA would have distinct off-target sites, eliminating its effectiveness as a control. Since off-targets...

  7. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...transformation due to background re-circularization and contamination from uncut plasmid. 4. Transformation Transform... higher competency cells. Additionally, if your final product is going to be very large (>10kb) you might...DNA and lets other materials pass through; and, finally, selectively elute the plasmid DNA from the column...

  8. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...of dsRNA. In plants, CRISPR technologies are predominantly used to disrupt function at the level of DNA...Darren Gilmour at the University of Zurich. He is fascinated by how organisms exquisitely and robustly self-organize...Interference System in Physcomitrella patens Reveals a Dominant Role of Augmin in Phragmoplast Microtubule Generation...

  9. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...be analyzed with a paired t-test, because they eliminate subject-to-subject variation (the test assesses...tests exist to identify significant pairwise combinations – Tukey’s test, Newman-Keuls, Duncan’s multiple...Comparison of Multivariate Means across Groups with Ordinal Dependent Variables: A Monte Carlo Simulation Study...

  10. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...antibodies here. Recombinant antibodies Similar to monoclonal antibodies, recombinant antibodies are a...from naturally-occurring immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences...
Showing: 461 - 470 of 772 results