We narrowed to 821 results for: LAS

...you the laser configuration. In general, if two fluorescent markers are read by the same laser, they will... markers you intend to use and your cytometer’s laser configuration. Your flow core or an online manual...reads out the fluorophores FITC and PE on the same laser, you will need to compensate between the two of ...your flow cytometer reads them out on different lasers, you should not need to compensate between them...

...transcription from the accessory plasmid (AP) encoding phage gene III. The accessory plasmid library contained all...PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the...doi: 10.1038/nature26155 PubMed PMID: 29512652 Plasmids from this paper are available at Addgene.  Additional...Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn about CRISPR History...

...cells. FPs classified by the emission color (emission wavelength range) FPs are usually classified by emission... relevant mutations, check out Marcy's post from last week on GFP. Additionally, many other FPs have been...emission. For example, if your microscope has only two lasers, at 488nm and 561nm, you will not be able to use... comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent Protein (GFP) Three Review...

...outlets have addressed CRISPR frequently over the last several years, but lay readers may be less familiar...is being done has changed significantly over the last decade. He describes the detection of gravitational...and cheap distribution of the newest CRISPR-Cas plasmids, has been a key driver of the technology.” As ...competitiveness of U.S. science and technology.” This last section of the book, Future Science, describes the...research. Even if Zimmer was able to rewrite the last chapter of his book today--the day you are reading...

...repair mediated by uracil glycosylase (Figure 1). The presence of a uracil glycosylase inhibitor (UGI) minimizes...all CRISPR plasmids and resources at Addgene Find CRISPR base editors Find CRISPR plasmids for plants ...smaller vectors suitable for transient assays using protoplast transformation. Cytosine base editors for plant...

...cells; however, Mycoplasma species do not possess cell walls. As a result, Mycoplasma are intrinsically...resistance are resistant to three or more different classes, or types, of drugs used to treat them. Very often...fantastic example of intrinsic resistance would be Mycoplasma species’ resistance to beta-lactam antibiotics...intrinsically resistant to this class of antibiotic. Acquired resistance can be the result of one bacterium sharing...

... either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic RFP in a specific cell type. The Confetti...et al., 2011) labels Drosophila neurons at the neuroblast stage, meaning that neurons of the same lineage...a specific tissue. XFPs are introduced using a plasmid construct called Zebrabow-GateDest containing an...introduced in vivo either by electroporation of plasmids, or using AAV vectors. The Imai lab also developed...

...Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids ... cellular processes by transforming cells with plasmids encoding aptamer-tagged RNA and later introducing...aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment...photostability of the Corn-DFHO complex. For dessert (last but not least), the only fruit-named aptamer, Mango...

...for future preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections...finger nucleases to remove exon 51 in DMD patient myoblasts. They observed a 13% removal rate of exon 51, ...gRNAs to delete exon 51 or exons 45-55 in patient myoblasts; when injected into DMD mice, these cells expressed... to ascertain how long the rescue phenotype will last, and more importantly, if it does extend lifespan... Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your...

...research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0...1.0, 1.1, 2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial... of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how...