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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 814 results for: LAS

Showing: 471 - 480 of 814 results

  1. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...cells; however, Mycoplasma species do not possess cell walls. As a result, Mycoplasma are intrinsically...resistance are resistant to three or more different classes, or types, of drugs used to treat them. Very often...fantastic example of intrinsic resistance would be Mycoplasma species’ resistance to beta-lactam antibiotics...intrinsically resistant to this class of antibiotic. Acquired resistance can be the result of one bacterium sharing...

  2. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ... either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic RFP in a specific cell type. The Confetti...et al., 2011) labels Drosophila neurons at the neuroblast stage, meaning that neurons of the same lineage...a specific tissue. XFPs are introduced using a plasmid construct called Zebrabow-GateDest containing an...introduced in vivo either by electroporation of plasmids, or using AAV vectors. The Imai lab also developed...

  3. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids ... cellular processes by transforming cells with plasmids encoding aptamer-tagged RNA and later introducing...aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment...photostability of the Corn-DFHO complex. For dessert (last but not least), the only fruit-named aptamer, Mango...

  4. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...for future preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections...finger nucleases to remove exon 51 in DMD patient myoblasts. They observed a 13% removal rate of exon 51, ...gRNAs to delete exon 51 or exons 45-55 in patient myoblasts; when injected into DMD mice, these cells expressed... to ascertain how long the rescue phenotype will last, and more importantly, if it does extend lifespan... Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your...

  5. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0...1.0, 1.1, 2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial... of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how...

  6. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...recombinant antibodies (rAbs), which are produced from plasmids and have very little batch-to-batch variation.... of sequence can be done from relatively cheap plasmid sequencing. While rAbs are monoclonal, recombinant... buffer than proteins easily available in the cytoplasm or whole-cell lysate. A harsher, SDS-containing...However, these systems are expensive, generate plastic waste, and limit the amount of optimization that...temperature for 1–2 years and are available in formulas specific to your choice of detection methods. ...antibody required. Reporters There are two main classes of reporters: chemiluminescence enzymes and fluorescent...

  8. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization...organization (now with more than 50% international plasmid distribution!) Chapter 8 - Opportunities to create...

  9. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...can request any of over 3,000 eligible AAV cargo plasmids in the Addgene repository to be packaged in your...Request With Packaged on Request, you can find your plasmids and your viral packaging service in the same place...packaged on request process, simply navigate to your plasmid of interest. If it’s eligible for packaged on request...special processes involved; simply request your plasmid of interest in your desired serotype, place your...

  10. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... there? A typical biomedical postdoc fellowship lasts five years. Most universities and institutes annually...feel like they've made enough progress since the last presentation. Yet, PFs need to tell and sell their... practice. If you simply recycle slides from the last time you gave a presentation to a specific group...trainees, communicate your expectations, and teach classes. Therefore, seek out management and conflict resolution...quality control of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent proteins...
Showing: 471 - 480 of 814 results