We narrowed to 884 results for: ica

...conventional microscopes and give a similar optical quality (magnification of 140X and 2 micron resolution). I... science communication and outreach - if you'd like to learn more about Science communication check out...listening to talks all day. But nope, not at the American Society for Microbiology 2018 Microbe meeting....biomolecule-based dyes. This technology is widely applicable as bacterial expression of dyes allows dye visualization...Bacterial Genome Engineering Learn about Science Communication using Twitter  Resources on Addgene.org Check...

...Balancing research with medical practice as an MD/PhD. Darrel does 20% clinical work 80% research and was...for Regenerative Medicine at Boston University Medical School. Darrell’s research focuses on the lungs...Center for Regenerative Medicine, and within the biomedical research community. Along these lines, Darrell...Pathology and Laboratory Medicine. He attends in the Medical Intensive Care Unit, the Pulmonary Consultation...the Pulmonary outpatient clinic, all at Boston Medical Center. In addition he conducts basic stem cell...Regenerative Medicine of Boston University and Boston Medical Center. Special thanks to our sound engineer/producer...

...variety of species, cell types, and research applications. Labeling neurons with Brainbow The Lichtman...mOrange2, EGFP, and mKate2 (red) to solve the problematically low fluorescent intensity of previous versions... Brainbow 3.1 and 3.2 (described in the same publication) are unique because by default, they are not ...level of these XFP is not strong enough for all applications. To resolve this, similar systems have been ...developed a version of Tetbow that incorporates chemical tags instead of XFP to optimize it for tissue ...architecture of a fluorescent neuron is visible. Chemical tags are more resistant to tissue clearing methods...neuronal circuits with fluorescent proteins and chemical tags." eLife 7 (2018): e40350. PubMed PMID: 30454553...

...insights into the complicated physical organization of the brain and other biological systems. Interested...deletions are defined using 2 loxP variants (only identical loxP sites can mediate recombination.) The deletions...of farnesylated derivatives enables labeling of delicate axonal and dendritic processes not previously ...Between Brainbow Systems   Brainbow variants and applications beyond the mouse brain In addition to improving...temporal control without the need for germline modification, and enables Brainbow to be used in a variety...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Snippert HJ, van der...

...content of this article. Biosensors (‘biological sensors’) are biological tools that monitor a process or detect...a reporter signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent..., and you should always check the associated publication for the specifics of your chosen plasmid. Types...Experiments Experiment  Detection Method Notes Quantification of a process Plate-based assays (plate reader...components of individual cells Separation/identification of fluorescently-labeled components Fluorescence-activated... Jessica Welch...

...attended both online and on site courses with the American Society for Microbiology (ASM) Scientific Writing...of any scientist’s career. We use writing to communicate our findings and share them with the world. If...simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts and...field of microbiology with multiple high-profile publications. My classmates consisted of graduate students...sessions and reinforced our newfound knowledge with practical understanding of how to apply it. The personal...well as being critiqued added an extra level of application not present in the online class.   Four ways ...paper will be generated. After you complete a publication quality figure, produce the figure, its legend...

... some of the drawbacks. Specifically, viruses are able to infect and replicate in host cells, but they...gene therapy using Sleeping Beauty to genetically modify clinical-grade T cells to target CD19. Immunol... though not all, of these defenses. For some applications of genome engineering - such as certain forms...to integrate. Additionally, the TA sequence is duplicated near the gene of interest insertion site.   The...Anderson Cancer Center presented results from a clinical trial using the Sleeping Beauty transposase as...

...the less acknowledged perks of scientific and technical training is that these educational paths prepare...Foundation study showed that 57% of PhDs in US Biomedical workforce will NOT go into “traditional” academic...tech transfer, management consulting, science communication, policy and the diverse options in nonprofit...science is much like industry but the “product” is publications—the pressure to produce is the same You get ...and teach students Collaborations may be less “complicated” - for example, easy to initiate a collaboration...is different about non-academia? Scientific publications are a nice to have, not a need to have Must ...every 3-6 years—a short and exciting ride Complicated matrix structures are common Small integrated...

...protein is (localization) and how much there is (quantification). But what if you’ve got several interesting...inactivate the fluorophores in the sample, either by chemical treatment or photobleaching or by stripping out...primary antibodies that have been validated in IF applications (find IHC validated or ICC validated antibodies...yourself stuck with red-green images generated automatically from the microscope software, you can always...it, the secondary antibody may be binding non-specifically – try optimizing your blocking and washing conditions...informative image-based figures for scientific publications. PLOS Biology, 19(3), e3001161. https://doi....Germain, R. N. (2020). IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial...

...generation when bound by the dL5** FAP.  The iodine-modifications also shift MG-2I’s excitation wavelength to ...FAP-TAP system works. Find the Plasmids from the Publication Here Light-induced cell ablation with MG-2I-dL5...the main advantages of MG-2I-dL5** over other genetically encoded photosensitizers is that it works with... ROS because NIR light doesn’t excite natural biological chromophores found in cells.  He et al also show...compared MG-2I-dL5** head-to-head with KillerRed, a genetically encoded red fluorescent photosensitizer protein...Watkins, S. C., . . . Bruchez, M. P. (2016). A genetically targetable near-infrared photosensitizer. Nature...Chepurnykh, T. V., . . . Lukyanov, K. A. (2005). A genetically encoded photosensitizer. Nature Biotechnology...