We narrowed to 783 results for: INA

...protein is composed of an n-terminal DNA recognition domain and a mostly c-terminal nuclease domain. Feng Zhang's...Splitting wild-type Cas9 into n-terminal (Cas9(N)-2xNLS) and c-terminal (Cas9(C)-2xNLS) fragments facilitates...with different N- and C-terminal tags. Additionally, by using an adapted destination vector with a heterologous...bearing the "Bunch of Baby Spinach"aptamer, which is a brighter version of Spinach2, an RNA aptamer that fluoresces.... Bunch of Baby Spinach, or BoBS, consists of three tandem copies of the Baby Spinach core embedded in...with Broccoli Vegetable tags, including Spinach, Spinach2, and Broccoli, can benefit your RNA experiments... control of gene knockout or activation, the c-terminal cas9 fragment was fused with FK 506 binding protein...

... Other variants of Spinach include Baby Spinach, a miniaturized version of Spinach that retains similar... Hammond’s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified...was consequently brighter than Spinach. The initial studies using Spinach2 showed its ability to track RNA...imaging. Bunch of baby spinach, or BoBS, contains tandem repeats of Baby Spinach. It was developed in a...twice as bright as Spinach2 when bound to DFHBI. It’s also smaller than any form of Spinach - only 49 nucleotides...Addgene’s collection. Green fluorescent aptamers Spinach and Broccoli and their derivatives are aptamers..., but not quantitative, studies. Additionally, Spinach, the first aptamer identified to bind DFHBI, is...

...bacteria. In vivo, these recombination reactions are facilitated by the recombination of attachment sites ... in the donor vectors and the destination vectors prior to recombination, and it is exchanged with the...or by Restriction Cloning Destination vector attR sites for recombination; ccdB gene for negative selection...vector systems. With the appropriate entry and destination vectors, one can use Gateway to clone a gene ...vitro version of the integration and excision recombination reactions that take place when lambda phage ...attP) and the bacteria (attB). As a result of recombination between the attP and attB sites, the phage integrates...into the bacterial genome flanked by two new recombination sites (attL-left- and attR-right-, Figure 1)...

... primer on how Cre-lox recombination works. Advances in Cre-lox recombination have included inducible...coupling of Cre-lox with FLP-FRT recombination to generate additional recombination events, and the synthesis...excision results in one of three recombinations, and subsequent recombinations cannot occur. While the Brainbow...Brainbow-1.0 mice.) The combinatorial effect of multiple, independent recombination events leads to a rainbow...systems remain valuable to biology. Cre-lox recombination, discovered in the 1980s, is one of the most... loxP sites to specify distinct patterns of recombination. In 2007, Joshua Sanes and Jeff Lichtman, both...M-CFP (membrane tethered cyan). Without Cre recombination, RFP is expressed. Cre can mediate one of two...

...factor and promoter pairs referred to as binary systems. Binary systems are genetic tools that consist ...repressible element to this binary system (making this a repressible binary system). The GAL80 repressor...expression and largely defines the amount of recombinant protein that will be produced. Many commonly-...default ON to an OFF state, as well as repressible binary systems. In recent years, a number of more complex... previous post. In the past 30 years since the original Tet-Off system was described by Gossen and Bujard...inducible manner detailed in our previous post. While originally developed for use in mammalian cells, this system...consumption phase of yeast culture. Repressible Binary Systems GAL4/UAS In Drosophila or development studies...

...cytosine base editor by coupling a cytidine deaminase with the inactive dCas9 (Komor et al., 2016). These fusions...RNA adenine deaminases have been engineered and/or evolved to catalyze cytidine deamination instead of ...adenine deaminases. The team used directed evolution to create one from the RNA adenine deaminase TadA.   ...protein (catalytically dead or nickase) fused to a deaminase that makes the edit A gRNA targeting Cas to a ... and team wanted to force the cell to use the deaminated DNA strand as a template. To do so, they used...copying the base edit. Figure 2: Cytidine deamination takes place on the free strand of DNA and converts...created the Target-AID base editor using a cytidine deaminase from sea lamprey fused to Cas9 nickase (Nishida...

...how the Cre-loxP recombination system can be used to induce site-specific recombination events, and that...sites to induce site specific recombination events: Cre recombinase binds loxP sites, while FLP binds ...presence of Cre recombinase.   Once the SSR of choice is introduced, recombination can proceed either...any additional recombination events are impossible even in the presence of Cre recombinase. This plasmid...BL. 2012. Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression...orientation of the flanking loxP sites directs the Cre recombinase to invert, translocate, or excise a DNA fragment...such strategy--the FLEx switch--which utilizes recombination elements to turn off expression of one gene,...

...break down how different contaminants impact these ratios.  Protein contaminants Whether you are purifying...indicator of protein contamination, but a number of other potential contaminants alter this ratio as well...phenol in your sample.  RNA contaminants DNA samples can also be contaminated with RNA (and vice-versa)....Characteristic absorption spectra for pure DNA and likely contaminants found in DNA samples. The blue lines in B-D ...B-D represent the spectra for the specified contaminant, while the grey lines in B-D represent the pure ...pure DNA spectra for comparison. Each of these contaminants will have a slightly different impact on the ...alter the overall 260 nm reading, distorting the final concentration calculation and potentially impacting...

...protein genes, Cre recombination is required before fluorescence can occur. Cre recombinase can be used alongside...only expressed following a recombination event. Tissue-specific Cre recombination in this mouse generates...may acquire one of a variety of colors when a combination of XFP are expressed. Each cell remains the same...with Brainbow The Lichtman group developed the original iteration of this technology in 2007. Used to ...cells will fluoresce either red or orange until recombination occurs. Following the development of Brainbow...the default state of the transgene such that recombination is necessary to excise the non-fluorescent YFP...that express the construct but do not undergo recombination can be detected by immunostaining of the truncated...

... Keep your contaminants away While it’s likely that you’re the main source of contamination for your experiments... their original condition and can replace your working stock if it gets too old or contaminated. This ...hopefully help you keep a well organized lab and contamination free environment for successful experiments:... up to half an hour can help keep your hood contamination free, but you should keep in mind that UV light...precautions necessary to keep your cells from becoming contaminated. Wipe down everything that’ll be brought under...entering the hood is another great way to prevent contamination. This sounds rather trivial, but often solves...is in progress.Learn more about cell culture contamination in this post from the ATCC Declutter! Keep the...