... by loxP sites. While there is only a small loxP scar behind at the site of excision, this approach is...insertional mutagenesis, the presence of a genetic scar, and incomplete silencing of transgenes. Overall...antigen-1 (EBNA1) based episomes: These plasmids carry the origin of replication (oriP) element and the...5. González, Federico, Stéphanie Boué, and Juan Carlos Izpisúa Belmonte. "Methods for making induced pluripotent... pluripotent stem cells: reprogramming à la carte." Nature Reviews Genetics 12, no. 4 (2011): 231-42. ...Mouse Expression Vectors Human Expression Vectors Carey et al Hockemeyer et al Brambrink et al Maherali...

...politicized. It would be nearly impossible for a career scientist such as Zimmer to introduce any one of...otherwise follows science closely through their careers or hobbies, are unlikely to be surprised by many...been aware of these problems since I began my own career over 20 years ago, and I’m sure they were issues...here, but it’s unclear how much they will actually care. The section of the book I’d really like to press...they need to grasp the challenges you face in your career.  Acknowledgements: I would like to thank fellow...

...non-academic science careers Learn about mentoring Resources on Addgene.org Check out our careers page Watch ...Creativity Data analysis Listening Negotiation Career Planning Resources Cross team communication  External...Watch our career videos ... career...

...all experiments carried out on low passage cells and this means you can regularly discard the cells you ...Resistance and Metastatic Potential in Adenoid Cystic Carcinoma, Up-Regulation of pVHL along with Down-Regulation.... The main difference is the QC and validation carried out by the commercial repository. Repositories ...also provides standards and has laboratories to carry our independent quality control for a range of testing...

...Symposia Prepare Yourself for a New Career in Science with Our Career Readiness Posts Learn Good Management...paying them overtime accordingly. Read Additional Career Posts on the Addgene Blog How did this change in...immediately lose jobs; that tenured professors/mid-career scientists/junior faculty will all be most/least...Scientists Resources on Addgene.org Learn about Careers at Addgene ...

...Enterobacter sp., Erwinia amylovora, E. carotovora subsp. Carotovora, E. herbicola, E. rubrifaciens, E. stewartii...can replicate and stably maintain the genes they carry among organisms belonging to many different phylogenetic...self-transmissible, meaning that the plasmid is conjugative and carries the genes necessary for transfer initiation between...Caulobacter crescentus, Desulfovibrio vulgaris, Erwinia carotovora, E. chrysanthemi, Escherichia coli, Gluconacetobacter...

...editing can integrate DNA cargoes of 80–100 bp, PASSIGE can integrate cargoes over a hundred times that... Structural determination on magnetic beads for scarce macromolecules in heterogeneous samples. bioRxiv...transduce cells directly with ribonucleoprotein cargo. eVLPs contain no genetic material, which ensures... transduced cells, limiting off-target editing. Cargo size limits are also less stringent for eVLPs than...

...swapping out printer cartridges can place the spent cartridge in the large cardboard box in the copy area...area. These spent cartridges are returned to Xerox for recycle and reuse, reducing the need for the production...

...- listen to learn more about Crystal’s exciting career path, to gain insight into industry research, and...and to get advice on how to succeed in your own career. One pro tip from Crystal - be agile and willing...Novartis. The importance of mentorship in developing career skills. Outro Many thanks to our guest, Crystal...Scientists Series Learn about Turning Your PhD into a Career with Joanne Kamens' AMA Get tips on Writing a Great...

...MacLeod, and Maclyn McCarty’s experiment debunked the myth that protein is the carrier of genetic information...of our research.  Want the Genome Editing Trading Card seen in this blog post?Download high resolution ...10.1038/s41586-019-1711-4 Avery OT, MacLeod CM, McCarty M (1944) STUDIES ON THE CHEMICAL NATURE OF THE ...137–158 . https://doi.org/10.1084/jem.79.2.137 Carroll D (2011) Genome Engineering With Zinc-Finger Nucleases...

...are seen in the culture, it is a good option to discard the flasks (unless the source of culture is irreplaceable...the prevention steps start from you. If you are careful enough to provide a sterile environment for work...sneeze or cough. Wear gloves while working and discard them after use. Human contact can spread contamination...contamination, what do you do next? The best way is to discard the infected cell culture flasks. In cases where...contamination rather than eradicating it later. Don’t be scared when mycoplasma infects your cell cultures because...

...Sign up to receive our science career guide Learn about different careers in science communication Resources...without having to extensively use Google search. Be careful about hidden connotations behind a word. Non-scientists...ambassador, SfN community leader, and an early research career panelist at BioTechniques.     Additional resources...

...are tempted to seek out alternative evidence and discard inconvenient findings. Yet, we cannot prosper in...propaganda that limits access to full reproductive health care. And it’s needed most in low-income and minority...W – Mark Lucera I am a passionate scientist.  I care about my work because it's important to our health...I had no idea what to expect. Admittedly, I was scared. I worried that, because we were speaking out against...ever thinking that research scientist would be a career that could simply cease to exist. But then November...

...learn about the myriad of diverse, nonacademic careers that will be available once they have a graduate...other research project. You’ve met someone whose career interests you or you want to pursue someone doing...nonthreatening request to discuss experience, interests and career path. Because there is no demand for a favor, the...opportunity to learn. Additional Reading More "Career Advice for Scientists" posts  ...

...science career search.  You might also enjoy the other posts in the Addgene Blog Scientist Careers Series...of a job application. 3) Read job descriptions carefully Be open minded and be bold. You don’t have to ... careers...

...to isolate, multiply, or express the insert they carry in the target cell. These vectors allow you to test...approach of an autonomously replicating vector that carries either a wild-type version of YGOI to rescue a ...a microscope, we offer a collection of vectors carrying several fusion proteins. Pick your promoter, selection...can be expressed in fly culture cells like S2 and carry the metallothionein promoter (e.g. pMT-puro). Worm...

...field that you may want to work on in your future career, so the research topic will be more important. ...advisor that will support your growth in your science career. Will you be given opportunities to learn and practice...the PI support this path and help you pursue any career alternative? Listen to our podcast interview with...that will be a lifelong partner in developing your career in science.  Read other posts in our Science Mentors...

...patients or caregivers affected by the disease in question who are involved in online patient/caregiver communities...socioeconomic and/or racial/ethnic background, patient or caregiver status, geographic location, sense of humor, media...Activity Example audience  Talking about your career as a scientist underrepresented minorities in ...

... prototype to gain any real traction.  This can carry a heavy price tag, especially if you're working ... not value your potential contribution to the healthcare landscape.  You will find an extra layer of difficulty...name (P-H-D).  Finally, having spent your entire career in science, you are likely lacking the necessary...milabinc.com       Additional Resources Find more career posts on the Addgene blog Read our recent post ...

...mentors and, perhaps, asked someone to support your career development as your mentor. How do you make the...that you can reap rewards in the form of reaching career development goals. Check out Joanne's Reddit AMA...together and chatting with another person about their career path is a great start to a mentoring relationship...actionable steps (e.g. Meet 10 PhDs with interesting careers, find a summer internship, etc.). It is helpful...

...Schwiesow A leading cause of death worldwide, cardiovascular disease is driven by atherogenic lipoproteins...been shown to have a strong correlation with cardiovascular disease and indirect measurement of ApoB-LPs...concentration, Steven Farber and colleagues at the Carnegie Institute for Science have developed the LipoGlo...to aid in the understanding and treatment of cardiovascular disease. Find the LipoGlo plasmids at Addgene...

... of researchers special: 1. Stem cell scientists care deeply about ethics Ethics must play a prominent...at scientific conferences. In my nearly 20-year career in science, I have never attended a scientific ...Researcher Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent...alone takes nearly a year. Addgene depositor Juan Carlos Izpsua Belmonte said it took the joint effort of...

... a lab that will be a good fit for you and your career ambitions. First, how does one stand out amongst...interview process. What if I want a non-academic career path?  If you are considering this, make sure to...will want in year 6. Have lab alumni taken diverse career paths? Ask other lab members how the advisor treats...inquiries. If you know you would like to head for a career in pharma or biotech, this is one case where the...

...materials to specific destinations, haul a variety of cargo, and even handle a variety of terrains. They are...infection) and delivering a genetic payload. This cargo is typically their own genome, but this step can...cell) can be tuned so that the copies of library cargo delivered are controllable. For many screening applications...essential to deliver a single copy of your library cargo to isolate the effects of each variable being manipulated...

...application to assess your eligibility, interest, and career fit. Most internships share a common set of criteria...potential, and drive. Read the associated prompt carefully, plan your response, and let your inner wordsmith...applying to several opportunities. In an age of scarcity, the rational human being will tend to clinch ... Check out other tools and resources for early career researchers Applying for jobs? Check out these ...

...Regenerative Biology is to understand the basis for cardiovascular and metabolic human diseases. They do that ...efficiently generate human stem cells edited to carry mutant versions of 15 different disease-associated... target a large number of genes involved in cardiovascular and metabolic diseases. We are knocking out... type relevant to the disease in question – cardiomyocytes, adipocytes or neurons, for instance – to see...

...Although it goes without saying that one should take care whenever doing any type of DNA or RNA purification...purification to avoid contamination, take extra care when performing RNA extraction. RNA is inherently not as ...problem with no-kit RNA extraction methods is the carryover of DNA that can potentially complicate results...extracting, to only take the aqueous layer to avoid carryover of DNA from the bottom, organic layer. Another...

...that’s an important soft skill to carry into the next phase of your career.  Happy defining! Additional...other transferable skills Find all of our science career related blog posts Read our science communication...communication blog posts Resources on Addgene.org Find careers at Addgene ...

... the Institutions” promotes gender equality in careers through structural change in research organizations...hormone therapy resulted in 76,000 fewer cases of cardiovascular disease, 126,000 fewer breast cancers, and ...fractures. The overall economic savings to the US healthcare system was $35.2 billion (or $140 per $1 invested...Extracurricular Activities for a Strong Science Career Path Additional Resources Check Out Vidoes of...

... about a Variety of Science Careers Resources on Addgene.org Find Career Opportunities at Addgene   ... - securing higher salaries. This decision will carry over into other aspects of postdocs’ lives. We know...plans and budgets working around issues such as childcare using money from their increased salaries. In ...