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We narrowed to 610 results for: STO;

Showing: 201 - 250 of 610 results

  2. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...science, dating back several hundred years, with historical ties to psychology and philosophy (the mind-body...in a certain type of behavior (for a review see Stoica et al., 2013). Vector delivery may also be achieved...service for their behavioral studies. References Stoica L, Ahmed SS, Gao G, Sena-Esteves M. Gene transfer...

  4. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ... In the US, UC Santa Cruz, MIT, UW Madison, and Boston University offer science communication programs... and money to a full-time graduate course, UWE Bristol offers an intensive 4-day Masterclass in science...or upload your data to Zooniverse. The Natural History Museum has downloadable resources to enable you...

  5. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...so many scientists who were potential Addgene requestors and depositors. China is in one of the top 10...good job of seeing tRNAs because they contain “hard stop” mutations. New techniques used by the Lowe lab ...can view public circRNA datasets and download the custom python scripts needed to discover circRNAs in RNA-seq...

  6. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...Women’s Hospital/Harvard Medical School and then Boston University (institutions where I’ve worked as a...became interim Chair automatically as it was an understood expectation of any Vice-Chair. Following an official...Juliet Moncaster is an Assistant Professor at the Boston University School of Medicine and is the former...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...human genes and are recommended for use in immunohistochemistry (Figure 5). Anti-MAPT [AT8] specifically...Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant..., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org...

  8. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...course, you will want to make sure that your final stocks only contain rAAV. To minimize contamination, use...preparation.  rAAVs  Now your AAV virions are ready to store or use. They are quite stable and will withstand...Londono, L., Arjona, K., Parianos, M., Truong, V., Rostonics, E., Hernandez, A., Boye, S. L., Boye, S. E., ...

  9. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...spectral flow and can be thought of as a series of histograms flipped onto its side and compressed into a heat...for rare cellular markers, beads can sometimes distort the fluorescent signature upon antibody binding...: Secondary AntibodiesAntibodies 101: Buffers, Storage, and Conjugates Resources on Addgene.org Addgene's...

  10. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...methods. First, its modular design allows for customization with strain-specific promoters, antibiotic resistance...species where its modular nature allows for the customization of guides, promoters, selection markers, and...(2005): 443. PubMed PMID: 15908923. Johnson, Christopher M., and Alan D. Grossman. "Integrative and conjugative...

  12. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...approach gives me a bit of flexibility since I can store the cell lysates at -80 °C for up to 6 months before... completed in one long day, or the virus can be stored at 4 °C and buffer exchanged the next day. Refer... comments below! Special thanks for Dr. Dan Stone and Harshana de Silva Feelixge at Fred Hutchinson...

  13. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ....io beta platform for sharing, annotating, and storing life science protocols. Since we began sharing ...management and you want the works: organizing reagent storage and inventory, managing orders, tracking students... scientists workflow in mind and can offer a one-stop solution to your lab's organization and management...

  14. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...biology also falls within this section. The GFP story has its own Franklin in Doug Prasher, who originally...describes the detection of gravitational waves--a story I was much less familiar with as a biologist--as... feel slightly dated by next week.   A stepping stone When I discussed The State of Science with the five...

  15. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...for a scientist. I think that establishing this history helped demonstrate that a) I was a real person ...which demonstrated to the students that scientific history was very much in the making. One of the exciting... in an exciting, tumultuous time in scientific history. Whatever your reasons, let us know how it goes...

  16. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...using and whether you are inoculating from a frozen stock or a colony from an LB plate. In my hands, a 5 mL...culture can be used for colony PCR and glycerol stocks. Dip/drip plants more than once: Dip your plants...University. She studies cell fate maintenance in the stomatal lineage and has made countless transgenic plants...

  17. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ... Commons license.  Histograms  For analysis of a single marker, a histogram showing frequency of events... “true” signal.  Figure 8: A series of histograms from Alyamani et al., 2018, showing number of...

  18. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...frustrating it can be to get a “bad batch”); Customizable sensitivity and detection - since they frequently...changes after target binding, aptamers are highly customizable in terms of how they actually “report” as biosensors...a higher melting temperature than its Spinach ancestors, and has a lower magnesium dependence. Though ...

  19. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...like the cold or flu) Overuse of antibiotics in livestock farming Lengthy courses of treatment with antibiotics...those bacteria that are partially resistant. If you stop a treatment early, you may be un-wittingly selecting...used in the clinical laboratory. What can we do to stop the emergence of antibiotic resistance?  The problem...

  20. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...college I studied development and evolution of histone genes in Strongylocentrotus purpuratus, the spiny...Discovering ways to culture, propagate, transform, store, and share all of these new organisms continues ...promiscuous and the gene that is responsible is understood. It’s possible to actually silence this gene ...

  22. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...different codons that encode 20 amino acids and three stop codons, meaning that the same amino acid can be ...codons that encodes the 20 amino acids and three stop codons. (B) Example of codon usage among different... References Athey J, Alexaki A, Osipova E, Rostovtsev A, Santana-Quintero LV, Katneni U, Simonyan V...

  23. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    Published
    Sept. 16, 2025, 1:15 p.m.
    ... clinical application in under a decade is an astounding feat of public investment and collaborative scientific...effective treatment. And base editing advances won’t stop here. By coincidence, KJ’s genetic sequence was ...., Correia, B. E., … Kleinstiver, B. P. (2025). Custom CRISPR-Cas9 PAM variants via scalable engineering...

  24. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...at the Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research institute, partnered...will also work in immunoprecipitation and immunohistochemistry. Table 1: IPI antibodies  Antibody ...integrin antibodies comes from IPI’s work with the Boston Children’s Hospital and Harvard-based lab of Tim...

  25. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...a room, but still have control over focus, zoom, stop/start record, and allow me to see an image.  Remote...control some basic functions, including starting and stopping recording).  Test: It was unbelievable how well...the beating heart of Addgene. It’s why we exist, storing plasmids, and distributing them to labs that need...

  26. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...modification with fatty acyl chains. For instance, myristoylation requires an N-terminal consensus sequence (...variants and check the functionality of the fusion (Mastop, 2018). Summary GFP is an all-round useful genetically...21619590. PubMed Central PMCID: PMC3129320.  18. Mastop, Marieke, et al. "A FRET-based biosensor for measuring...

  27. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...to capture ideas, write down certain notes or brainstorm, but we have to admit that paper-based record... and included hundreds of researchers. The full story is available here. Our questions focused on researchers...all types of data, which enables researchers to store everything in one place. From pictures to excel ...

  28. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...2006): 703-9. PubMed PMID: 16582903. Potter, Christopher J., et al. “The Q system: a repressible binary...from this publication at Addgene. Wei, Xing, Christopher J. Potter, Liqin Luo & Kang Shen. “Controlling...s41598-022-07852-7 De Boer, Herman A., Lisa K. Comstock & Mark Vasser. “The tac promoter: a functional...

  29. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ... in your specific experimental context (Pillai-Kastoori et al., 2019). Tissues or cell lines other than...and references References Aydin S (2015) A short history, principles, and types of ELISA, and our laboratory...://doi.org/10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache ...

  30. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...the recombined product, you’d microinject into blastocysts to be implanted in pseudopregnant female mice...s a guide that directs cleavage right before the stop codon!  Figure 3: How to design a C-terminal...guide would be selected, since it cleaves at the stop codon TAG. Design your repair template with ...

  31. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...and whose structures are just beginning to be understood. Therefore, in addition to their utility in the... strain that you can use for long-term library storage. After overnight growth, scrape up, pool and extract...during one of these steps is also a convenient stopping (ie. going home and eating and sleeping) point...

  32. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...adapt. By the end of my three months, I fully understood why parasitology roles in the field require experience... available at a small science non-profit doing customer service (recruitment agencies often don’t tell...I was also surprised to learn that I enjoy the customer service side of my job. Because of my background...

  33. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ... viruses were produced in high-titer retroviral stocks generated by the transfer of a retrovirus into ...gene (packaging plasmid), and 3) the vesicular stomatitis virus G protein (VSV-G) that improves the stability...24h after transfection. This supernatant can be stored at 4 °C or can be directly added to your target...

  34. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...This is a good option for applications like immunohistochemistry on human tissues, when (1) other strategies...correspond to protein levels, so you perform immunohistochemistry with your antibody on these tissues (B)....mean it’s a bad antibody but more that it’s misunderstood and it could work great in another context! ...

  35. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...specific base sequences, but are sensitive to distortions in helix shape (22). Large purine:purine mismatches...enzymes.     References 1. Shuman, Stewart, and Christopher D. Lima. "The polynucleotide ligase and RNA capping...Jayakrishnan, Pravin A. Nair, and Stewart Shuman. "Last stop on the road to repair: structure of E. coli DNA ...

  36. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d ...performing quality control, and providing technical customer service. Team members are highly trained, and ...Jefferson dined alone.” There was a time in human history when an intelligent, motivated person really could...

  38. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...workhorse yeast — K. phaffii (formerly known as Pichia pastoris). If your goal is protein isolation, then the ...more MoClo? Check out Addgene's MoClo Guide! Customizing MoClo for your needs As you probably realize ...et al. “A Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol...

  39. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...Antibodies can be used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry). This could...bind to and inhibit the function of a protein to stop its effect within the body, or they can be used ...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

  40. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...leads to the first base transversion editor The story of base transversion editors starts with researchers...the SOX2 activation domain (SAD), which recruits histone acetyltransferase to open chromatin, improved editing... newly synthesized, templated sequence. And the story still isn’t over! Keep an eye out for our next post...

  41. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...creating flanking overhangs. These overhangs can be customized to link together multiple fragments, allowing...1x4; for 6 gRNAs, you’d use pX330A-1x6. This customization means you don’t ever need to clone in filler...plasmids allow expression of 2-5 gRNAs in zebrafish. Custom destination vectors are used depending upon the...

  43. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...Addgenie Eric Perkins attended the recent Keystone Meeting "Precision Genome Engineering and Synthetic...www.genome-engineering.org and http://www.egenome.org." At the Keystone Meeting Eric presented a poster with data comparing...

  44. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...including pseudorabies virus type 1, vesicular stomatitis virus (VSV; Beier et al., 2011), adenovirus, ...mechanisms of trans-synaptic trafficking are not fully understood and may be a form of transcytosis where the AAV...transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors. Proc Natl Acad Sci USA 108, 15414...

  45. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...elements from rabies plasmids to international requestors. We also offer export license applications for...processes, don’t hesitate to contact our friendly customer support team at [email protected].  Curious about...

  47. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...also take a look back on some fascinating Addgene history that you might not have known about. Here's the...Throwback Thursday (Blue Flame awards and Addgene history) Friday: Wrap up! To show the magnitude of plasmid...

  48. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...tradition in which we choose our teams and start brainstorming months in advance. Our teams comb the news (...year. They showed us their dedication to great customer support by dressing up as our Zendesk email system...

  49. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...our viral vector service Addgene has offered in-stock viral vectors since 2016, with the goal of providing...production and quality control processes as our in-stock service: ddPCR, purity testing, and sterility testing...

  50. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...total requests, total countries represented by requestors, year of first reagent sent, year with most reagents...Therefore, users with some knowledge of R can further customize the dashboard to their needs. I hope you find ...
Showing: 201 - 250 of 610 results