We narrowed to 746 results for: TRI

...This post was contributed by Patrick Miller-Rhodes from the University of Rochester Medical Center.  ...guest blogger Patrick Miller-Rhodes from the University of Rochester Medical Center! Patrick is equal parts...in large enough quantities (and the correct stoichiometric ratios) to generate useable data. What’s more...

...dependencies they identified and their associated ancestries. While there were some exciting relationships...Dependency Map) than lines from European or East Asian ancestries. These SNPs, however, were not accounted for ...racial bias. It is easy, even unintentionally, to attribute to an ancestry group what is actually an artifact...and references References Germline variation contributes to false negatives in CRISPR-based experiments...experiments with varying burden across ancestries. Sean A Misek, Aaron Fultineer, Jeremie Kalfon, Javad Noorbakhsh...

...generally referred to as Antigen Retrieval. I chose not to get into antigen retrieval here because it’s not quite...permeabilization reagents are detergents, such as Triton X-100, Tween-20, or saponin. Each detergent has...membrane-associated proteins in place. In contrast, Triton X-100 and Tween-20 are examples of non-ionic detergents...Permeabilizing with a non-ionic detergent, like Triton X-100, can lose membrane-associated proteins, but...

...This post was contributed by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital...patients with skin disorders for research and clinical trials, as it allows access to a larger number of willing...phenomenon has been recorded in past gene therapy trials and remains a safety concern in the clinical field...Furthermore, working with Sendai virus requires stringent biosafety measures, thereby increasing the costs...Using Serial Plating of Reprogrammed Cells by Centrifugation. Stem Cells Int, 2016. 2016: p. 1329459. PubMed...

...This post was contributed by Katherine Rogers, a postdoctoral researcher at the Friedrich Miescher Lab...processes—not just development—are available from strain distribution centers in the US, Europe, and China. In addition...pathways—such as those regulated by one-eyed pinhead—contribute to the “sculpting” of tissues and organs during...Katherine W. Rogers is a postdoctoral researcher in Patrick Müller’s group at the FriedrichMiescher Laboratory...El-Brolosy, Mohamed A., et al. "Genetic compensation triggered by mutant mRNA degradation." Nature 568.7751 (...

... Weakly electric fish are excellent systems to study for convergent evolution because electric organs ...leave them to their own devices. Chytrid fungi (Lillian Fritz-Laylin) Chytrid fungi are useful for the study...but there are few genetic tools currently for terrestrial frogs. Cnidarian-Dinoflagellate symbionts (Virginia...

...It is also worth noting that plasmids can be symmetrically incompatible, meaning that both plasmids are... from the cell with the same probability, or asymmetrical, where one plasmid lost from the cell at a higher... higher rate than the coresident plasmid. Asymmetric plasmid loss occurs for several reasons, including...high copy incompatible plasmids that lead to asymmetrical plasmid loss of the usually large, low copy ...: 15916596 Kamruzzaman M, Shoma S, Thomas CM, Partridge SR, Iredell JR (2017) Plasmid interference for...

...controlled by collections of neurons that are intricately wired into circuits through synaptic connections...weakly expressed. As these epitopes tags have no intrinsic visual signal, they cannot be detected directly...neurons. As compared to epitopes tags, FPs are intrinsically fluorescent and can therefore be used in live... interactions. Also, overexpression of FPs can trigger protein aggregation which is toxic for the cells...darkened chromophores. These probes are both intrinsically fluorescent and can be detected with highly ...

... This post was contributed by Ryan Watkins, Professor at George Washington University and developer of...may also benefit, such as researchers in other countries or ot­her disciplines, are even less likely to...research from other disciplines, or from other countries, to explore new connections to our research. So...hours of video were watched each month, in 39 countries, and in over 54 languages. While most scientific...citation rates to measure the impact of their contribution. It offers a 5-step process and several examples...

...This post was contributed by ATCC Outreach Scientist Nick Amiss. Cell culture in the present day Cell...major cell lines on public databases. Image attributions (from top to bottom) Database Center for Life...culture outlined in this article. Now many journals strictly require evidence of cell line provenance and identity...Nature Publishing Group recently reiterated its strict stance on the matter. Funding bodies such as the... at LGC Group, London, which is the European distributor for ATCC. He studied biochemistry at Warwick ...

...This post was contributed by guest blogger, Stephanie Hays, a scientist with a passion for photosynthetic...States government? Politics and science are intrinsically linked: governments exist in reality and science...said, government must have the same fundamental attribute that enables science: “humility in the face of...supporting many different positions. It would be detrimental for them to be homogenized or thought of as just...Scott Pruitt says carbon dioxide is not 'primary contributor' to global warming The New York Times: With Trump...

...DNA, important when trying to cut with certain restriction enzymes (ex: ClaI or XbaI) dcm DNA cytosine...DNA, important when trying to cut with certain restriction enzymes that are methylation sensitive. dnaJ...relA1 Relaxed phenotype, mutation eliminating stringent factor Allows RNA synthesis in absence of protein...plasmid maintenance, blue/white screening, partly restriction-deficient; good strain for cloning repetitive...methylated, allows for methylation sensitive restriction enzymes to cut the plasmid after preparation....

...labs have deposited plasmids to Addgene and we distribute over 130,000 plasmids in 2014. Thus, we are in...on CRISPR technology, for example, the authors Patrick Hsu, Eric Lander, and Feng Zhang acknowledge the...accelerated through a combination of open-source distributors such as Addgene, as well as a number of online...with data comparing Addgene global deposit and distribution trends for Zinc Finger, TALE, CRISPR, and SynBio...

...extracellularly-anchored horseradish peroxidase (HRP) in striatal astrocytes and neurons to label surface proteins...astrocytes and neurons (CS SPAN) featured extracellular matrix proteins, cell adhesion molecules, transporters...to the nuclease, and excises the DsRed-STOP to trigger expression of EGFP. Figure 2: Schematic...as a mass that starves the cancer of essential nutrients and alters the tumor microenvironment (Nguyen ... cells utilizing glucose to the exclusion and detriment of the cancer cells.  Figure 5: Implantation...

...These classical 'undruggable' targets are often intrinsically disordered and structurally complex. The tumor...first identified that in a set of patients with gastric cancer (GC), TP53 was one of the most frequently...with Addgene's quality control, support, and distribution capabilities. Give HiBiT a try and see your ...Site-Linked Chemosensitivity in Early- and Late-Onset Gastric Cancer. Cancer Medicine, 14(8). https://doi.org...https://doi.org/10.1038/nmeth.4344  Peach, C. J., Kilpatrick, L. E., Woolard, J., & Hill, S. J. (2021). Use...

...announce that we recently acquired the ability to distribute plasmids with the piggyBac™ transposon. These...piggyBac™ transposase (available from a licensed distributor) allow you to quickly transfer a DNA sequence...transposon vector to one of many TTAA sequences distributed throughout the genome. We encourage you to deposit...expand this useful resource. While Addgene cannot distribute plasmids with the piggyBac™ transposase itself...from the folks at Transposagen. This post was contributed by Amar Singh and Mathew Goodwin from Transposagen...

...preparation for our upcoming affinity reagent distribution launch - and we're excited to say our long naming...to help us with our upcoming affinity reagent distribution launch! There was only one problem - we didn't... biggest discovery yet: Addgene will soon be distributing affinity reagents. Name Our New Antibody Friend...luck and happy naming!   Fig. 2: The mascot trio!     ...

...This post was contributed by guest blogger Eleanor Wang, a Research Specialist in Patrick Hsu's lab at ...problems. Biomedical research, for instance, contributes heavily to medical practice, and understanding... and applied. The current pandemic serves as a striking example of the applicability and importance of...Eleanor Wang is currently a Research Specialist in Patrick Hsu’s lab at UC Berkeley. She will be starting ...

...This post was contributed by Kartik Lakshmi Rallapalli, a graduate student at the University of California...manipulate it. The term genetic engineering itself is attributed to SciFi writer Jack Williamson who used it in... Zinc finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding...template.  Each zinc finger recognizes a nucleotide triplet and when several ZFNs are strung together, it can...the ZFNs, but instead of recognizing nucleotide triplets, the components recognize individual nucleotides...

...This post was contributed by guest blogger Søren Hough, a Biochemistry PhD candidate at the University...mismatch along the length of the gRNA given the stricter sequence requirement at the terminal 3’ PAM site... Dillard, and Ayokunmi Ajetunmobi for their contributions to this work. Søren Hough is a Biochemistry ... mismatch cleavage assays to detect mutations triggered by engineered nucleases." G3: Genes| Genomes| ...PMID: 27087594. 16. Tycko, Josh, Vic E. Myer, and Patrick D. Hsu. "Methods for Optimizing CRISPR-Cas9 Genome...

...scientists at 5,200 institutions in 82 different countries. We continue to ship out more plasmids each year...beginnings in 2004 to today, you can see how plasmid distribution has grown. Additionally, more and more scientists...research. *2015 total reflects the number of distributed plasmids as of March 26, 2015. How does Addgene... our depositors to allow us to help store and distribute their plasmids. The partnerships and collaborations...

...SaCas9 genome editing plasmids String assembly gRNA cloning (STAgR) String assembly gRNA cloning (STAgR)...studied Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal DNA cleavage at...STAgR), developed by Stefan Stricker’s lab, is a single step gRNA multiplexing system that generates vectors...

...component of Golden Gate and GreenGate are type IIS restriction endonucleases. They are like other endonucleases... should in principle work in any plant species. Tricks of the trade Lohmann does have one special tip ...their own lab: be very careful with the type IIS restriction endonuclease of choice, BsaI, which can be very... will get busy with GreenGate, producing and contributing more modules into the public domain in the process...

...cells that have been infected with a microbe, to trigger immune cascades that clear these infections. Anything...letters and be able to guess whether or not that string of letters is a part of the word you’re searching... that you want the antibody to recognize. This triggers an immune response within the animal, and its ...antibodies (i.e., immunofluorescence), or by a colorimetric reaction facilitated by an enzymatic reaction... antibodies for neuroscience research from the Trimmer lab ...

...detergents such as triton-x, saponin, or tween. Take care when using strong detergents like triton-x and tween...fluidics system they pass through a flow cell which restricts the size of the stream and forces the cells to...cell expressing low levels of the protein. The electrical system converts the fluorescence intensity to...you would need a way to determine the relative contribution of yellow and orange from FITC and that from...

...This post was contributed by Doug Richardson, Director of the Harvard Center for Biological Imaging and...focus fluorescence from reaching a detector or restricting fluorescence to specific excitation volumes to...reduction in signal intensity. 2-Photon microscopes restrict excitation (and bleaching) to a single focal point...microscopy uses a number of optical and chemical ‘tricks’ to turn subsets of fluorescent molecules on and... a large axial range. 2-photon imaging, which restricts excitation and photobleaching to the focal plane...

...various grades of plastic lumber and distribute it to industrial and residential markets. Rethinking our...this, we can use the ACT label, which is an eco-nutrition label for laboratory products that emphasizes ...typically is destined for a landfill. However, Triumvirate Environmental developed Red2Green, a program ...

...in the same vector backbone with very similar restriction enzymes. You can essentially cut and paste quite...mind that a very simple sequential or double restriction enzyme digest will open up possibilities exponentially...these vectors to the scientific community and contribute in a way that everyone could utilize what we ...really the one who had been very adamant that we distribute to as many researchers as we could, and Addgene...

...the first gRNA using restriction enzyme BbsI and the second gRNA using restriction enzyme BsaI. If you’... of gRNAs Golden Gate Assembly uses Type IIS restriction enzymes, which cleave outside of their recognition...These expression vectors each contain Type IIS restriction sites flanking the promoter-gRNA construct, but...oligonucleotides. These plasmids contain Type IIS restriction sites that flank the promoter-gRNA constructs...

...programs either. But with a little Googling and some trial and error, I found some free design software that...number of drawing tools, so it’s harder to draw intricate figures, like a brain or a mouse. Vectr Vectr ...and digital articles where it’s easier to give attribution, than for journal publications. Google Image ...includes images that can’t be reused without attribution, despite filtering for images labeled for reuse...

... al., 2020). A transducer complex consists of a trimer of three subunits: alpha, beta, and gamma (Gα, ..., Gβ, and Gγ), collectively known as a heterotrimeric G protein. This complex converts a signal received.... When inactive, these subunits are found in a trimeric complex bound to the intracellular surface of ...called BRET2. Figure 1: TRUPATH measures heterotrimeric G protein dissociation by bioluminescence resonance...

...let us know in the comments. Note:Marcy Patrick contributed to the writing of this article. ... Short segment of DNA which contains several restriction sites allowing for the easy insertion of DNA....utilize one of a variety of cloning methods (restriction enzyme, ligation independent, Gateway, Gibson...

...fulfill our mission by generating revenue through distribution of research materials. Our small shipping and...generate revenue (as Addgene does through materials distribution). In fact, healthy nonprofits should have some...their revenue. A nonprofit organization cannot distribute profits to private individuals through stock ...request plasmids (via web interface, processes, distribution, and customer service) Addgene provides unmatched...

...This post was contributed by guest blogger Jon Backstrom, a biochemist in the Vanderbilt Eye Institute... have been immobilized on a reduced glutathione matrix. The advantage of a fusion protein is the efficient...provide a more relevant kinetic analysis, but the trick is to utilize a second immobilization method. One...available. Factor Xa loves calcium, but metal-chelate matrices, not so much. Maybe a calcium-independent proteinase...

...The triple T’s of biology are transformations, transfections, and transductions! In this blog we will...basics but are here because you want to know the intricacies of transductions, we've got you covered there... easy. Producer cell media can be collected, centrifuged to remove any producer cells floating in it, ...long-term (AAV). If you perform a lipid-based or electrical-based transfection, most of the nucleic acid ...

...! Archon1, a voltage sensor AAV Historically, electrical activity in the brain was measured with implanted...voltage reporter allows a way to monitor neuronal electrical activity through imaging. Since it’s illuminated...particular cell types. For example, the mDlx enhancer restricts expression to GABAergic interneurons. These vectors...pAAV-hSyn-EGFP *Also coming in our new 20 ul trial size! Voltage indicators Plasmid Serotype Name...

...protein to regulate cell growth dynamics Article contributed by Klaus Wanisch  Listen to the optogenetics ...visualizing RNA production and localization Article contributed by Tyler Ford  Listen to the riboglow podcast...developing new FPs - the mCarmine story Article contributed by Caroline LaManna  Listen to the mCarmine podcast...base-editing and Cas9 cleavage activity Article contributed by Angela Kaczmarczyk  Listen to the base editing...

...training progresses.  In From Academic Solos to Industrial Symphonies, an article well worth reading on ... notice or choice Always working on a team/in a matrix - can be frustrating for solo flyers Other people...—science can be surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others... years—a short and exciting ride Complicated matrix structures are common Small integrated teams, ...

...cloning) is a DNA cloning method that does not use restriction enzymes or ligase, and requires no post-PCR procedures...depicted as a green cloud) functions both as a restriction endonuclease and as a ligase by cleaving and ...Design standard primers (no need to add unique restriction sites on the ends) and amplify your sequence ... confirm the presence of your insert by PCR, restriction digest, or sequencing. Pro tips Do not add 5...

...This post was contributed by guest bloggers Lauren Celano of Propel Careers and Rachel Casseus, Esq. ...practice in that field. H-1B work-authorization is strictly limited to employment by the sponsoring employer...of the work of those in your field. Original contributions to the field that have had a major impact. For...of original scientific or scholarly research contributions in the field Evidence of authorship of scholarly...Addgene.org Careers at Addgene Global Plasmid Distribution ...

...This post was contributed by Deborah Sweet, Vice President of Editorial at Cell Press. Almost everyone...Communication is the issue Much of the problem can be attributed to communication, and in particular communication...(accession numbers, reagent details, author contributions, etc.) before their paper can be published. ...across life and physical science, and editorial contribution to the strategic development of Cell Press. ...

...of proteins for degradation using AID Article contributed by Alyssa Cecchetelli Auxin Inducible Degradation... of CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam CRISPR/Cas9 is widely used...channelrhodopsins for the optogenetic toolbox Article contributed by Susanna Bachle Anion-conducting channelrhodopsins...31346176. Using LADL to loop with light Article contributed by Eric Perkins Mammalian genomic DNA is not ...

...This post was contributed by Manon Eckhardt and Melanie Brewer from the QBI Coronavirus Research Group... these proteins are FDA-approved or in clinical trials or preclinical studies already. We are following...all viral genes are flanked by EcoRI and BamHI restriction sites - so cloning projects should be fun and...partnering with Addgene, who will be taking over the distribution to expand the network of scientists working ...

...This post was contributed by guest blogger Lydia Morrison from New England Biolabs. What is DNA assembly...standardized DNA constructs, but its use of Type IIS restriction enzymes results in scarless assembly. Finally...allowing fragments to be taken directly from restriction enzyme digests to DNA assembly. What makes it...containing regions of homology) cut from plasmids via restriction enzyme digestion, and used directly in DNA assembly...

... exploded so quickly, citing the first clinical trial with iPSCs with his collaborator Takahashi in 2014...perspective: Dr. Yamanaka talked about his clinical trial patients with advanced macular degeneration; Laurenz...presented work that is leading to promising clinical trials for ALS.   I have an uncle with Parkinson's, a ...first stem cell line that could be used in clinical trials. The quality control process alone takes nearly...

...also use electroporation, the application of an electrical charge to cells, to increase cell membrane permeability... integrated phage remains dormant until it is triggered to enter the lytic cycle.   During both of these...they observed genetic recombination between two nutritional deficient E. coli strains that resulted in a ...cells. Electroporation on the other hand uses electrical pulses to create transient pores in the cell ...

...charming nighttime displays, these insects have contributed an important tool to the scientific community... small (19 kDa) luminous shrimp (Oplophorus gracilirostris) luciferase and engineered to be extra bright...Luciferin ATP No Luminous shrimp(Oplophorus gracilirostris) Natural 455 19 Coelenterazine None Yes NanoLuc... NanoLuc®(derived from Oplophorus gracilirostris) Engineered  462 19 Furimazine None Optional AkaLuc...

...floats freely, depicted in the figure below. Once triggered by blue light, the CRY2 and CIB1 proteins heterodimerize...specificity as full-length Cas9 (flCas9). When triggered by blue light (470nm), paCas9-1 induced indel ...response to far-red light (730 nm). This nucleotide triggers the translocation of the protein BldD to the nucleus...constructs. This proof-of-concept model opens intriguing possibilities for optogenetic manipulation of...Gene editing Manna et al., 2019   NPPOC-caged trimethoprim (TMP) Cas9 fused to destabilizing domains (Cas9...

...resources. There are three types of cognitive load: intrinsic cognitive load is the effort associated with a...even the most enthusiastic of learners.  What contributes to cognitive load?  Everything! Here are some...specialized knowledge and context I have spent years striving to obtain?” If the answer is yes, you will need... or to gloss over some aspects when needed. The trick is to communicate your key points well enough that...

... on size is ultracentrifugation. Sedimentation equilibrium analytical ultracentrifugation yields an affinity...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...on the dimerization tendency in living cells. Strikingly, the OSER assay reveals that tagRFP does not ...