We narrowed to 137 results for: 11

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Aug. 27, 2015, 2:30 p.m.

Nanoblades are a CRISPR delivery tool. These Nanoblades are engineered MLV-like particles loaded with Cas9-gRNA ribonucleoproteins.

Sept. 26, 2019, 12:50 p.m.

Learn how you can use plasmids to teach students about functional genomics with your own "Pathways Over Time" project.

May 22, 2018, 1:38 p.m.

Summary of degron tags along with their applications, advantages, and several commonly used tags.

March 14, 2024, 1:15 p.m.

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Dec. 10, 2020, 1 p.m.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Feb. 23, 2016, 3:30 p.m.

Interview with Nicola Patron about her work in plant synthetic biology and her Golden Gate MoClo plasmid kit.

June 12, 2014, 3:30 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Aug. 28, 2025, 1:15 p.m.

Learn about evaluating DNA quality with Spectophotometry using a Nanodrop

July 1, 2025, 1:15 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

March 11, 2025, 1:15 p.m.

Learn about how Cascade-Cas3 can be used as a CRISPR tool to degrade large fragments of DNA.

Feb. 11, 2025, 2:15 p.m.

Magnetoresponsive proteins have seemed like a dream... but Andrew York's lab has developed and deposited one, opening the possibility of magnetoresponsive tools!

Sept. 24, 2024, 1:15 p.m.

Data-driven design of Golden Gate Assembly overhangs greatly increases the number of fragments that can be used in cloning.

Aug. 1, 2023, 1:15 p.m.

Marc Zimmer's book, The State of Science, examines broad issues in science including racism and sexism, peer-review, funding, CRISPR, and the politicization of science.

July 30, 2020, 1:15 p.m.

David Liu's lab analyzed how COVID-19 affected their plasmid distribution.

July 16, 2020, 1:15 p.m.

Learn all about CAPTURE - a new CRISPR-based technique to study regulatory elements in their native chromatin contexts.

July 2, 2020, 12:40 p.m.

Learn about aptamer-based fluorphores and how they can be used to brighten up your experiments.

April 11, 2017, 2:30 p.m.

Learn how you can use MXS-Chaining to generate complex, multicistronic plasmid constructs.

Feb. 7, 2017, 3:30 p.m.

Addgene interviews Hodaka Fujii of Osaka University to learn about his lab's enCHIP system, their new CRISPR tools, community involvement, and more.

Dec. 2, 2014, 7:23 p.m.

Multicistronic or bicistronic vectors contain IRES and/or 2A peptides to allow for coexpression of multiple genes from a single transcript.

Sept. 9, 2014, 8:20 p.m.