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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 292 results for: ADA

Showing: 81 - 100 of 292 results

  2. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...recombinase-based state machines and how they function. Adapting Toehold Switches to Detect Zika Virus The RNA-... understanding of Cas9 biophysics! Casilio: An Adaptive, Multitasking “CRISPR-OS” In this post, Albert...

  3. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...tool for enrichment of ABE-targeted cells By Ellie Adams Single base modifications through ABE or CBE's have... from hypersaline samples, were engineered and adapted for RNAi experiments for mammalian cell lines. ...

  4. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...researcher. There existed, therefore, a need for an adaptable tool that could continuously monitor multiple ...- Overview of the neural and gut fiber system (adapted from Sahasrabudhe et al., 2023).   To develop...

  5. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...adherent monolayer, though derivatives have been adapted for growth in suspension. Of the many derivatives... allows for large scalability, and can also be adapted for serum-free growth, which significantly reduces...

  6. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...nucleus and cleave the specified target. Figure adapted from D’Astolfo et al. In certain instances,...mammalian cells by osmotic lysis of pinocytic vesicles. Okada CY, Rechsteiner M. Cell. 1982 May;29(1):33-41. PubMed...

  7. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...high school classrooms, but it could be widely adapted and used in a variety of settings. If you are a..., if you are a fellow researcher interested in adapting your research ideas into hands-on educational ...

  9. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...Arabia -- as well as closer to home in the US and Canada. In fact, we received so many requests (175 and...imagine, it’s also been quite the administrative headache receiving constant email requests and tracking...

  10. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...career paths and things you can do to prepare. Adapt to WFH while parenting Keep your kids on a schedule... your work schedule as possible so that you can adapt to your kids' needs. Plan ahead. Think about and...

  12. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...RNA-seq was performed to map TFs to cell fates. (Adapted from Joung et al.)    This atlas linked TF ... color coded nominated reference cell types.  (Adapted from Joung et al.)    Putting MORF to work ...

  13. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from Figure 3 Colognori et al 2022.   Find CRISPR...temperatures, and in the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods ...

  14. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...with synthetic biology lab kits and lesson plans adapted from her own MIT curriculum. Drawing on current... BioBuilder book chapter and an illustrated, downloadable classroom poster — with both of these resources...

  16. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...intra-molecular annealing. Figure and legend from Okada, 2013.   scAAV If your gene of interest is truly...PMID: 20649475. PubMed Central PMCID: PMC2957237. Okada, T. (2013). Efficient AAV Vector Production System...

  17. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...for assembling the barcoded gRNA library pool. (Adapted from Wong et al., 2016). With a list of gRNA...assembling barcoded combinatorial gRNA libraries. (Adapted from Wong et al., 2016). With CombiGEM and ...

  18. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...in optogenetics, opening up the possibility of adapting GFP lines for optogenetics experiments in specific...transcription activation domains. Both systems are easily adaptable to neuroscience applications, including optogenetics...

  19. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...few steps. More recently, CRISPR technology has adapted Golden Gate cloning for inserting the appropriate...single gRNA-expressing plasmid, but it can also be adapted to express multiple gRNAs. Addgene has two Golden...
Showing: 81 - 100 of 292 results