We narrowed to 120 results for: ALC
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TypeBlog PostPublishedJune 1, 2017, 2:30 p.m....) respectively. The theoretical brightness is calculated by multiplying EC by QY (EC*QY, sometimes normalized...
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Screening for Successful Genome Editing with Digital PCR
TypeBlog PostPublishedMarch 30, 2017, 2:30 p.m....wild type target in each sample can be quickly calculated (details in [2]) and can be used to quantify ... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....site. A specificity ratio can be measured by calculating the on-target activity divided by the off-target... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....physical map distances of 2 and 75 Mbp, with the calculated fluorescent distances correlating with the previously... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....doi.org/10.21203/rs.3.rs-3188559/v1. Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....doi.org/10.1523/ENEURO.0171-19.2019Jendryka M, Palchaudhuri M, Ursu D, van der Veen B, Liss B, Kätzel D,... -
Viral Vectors 101: Virus Safety
TypeBlog PostPublishedJune 22, 2023, 1:15 p.m...., which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic acid, 10% bleach, or 1% iodine ... -
Hot Plasmids - October 2020
TypeBlog PostPublishedOct. 13, 2020, 1:15 p.m....AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb. ... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....INTRSECT project. Check out the new additions. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m...., 1997), was designed to measure intracellular calcium and published in 1997. Since that time, numerous... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m.... Lysine (D123K), lowers biosensor affinity for calcium.” Primary Vector Type The vector type should... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m....transformation efficiency, or more specifically calculated based on the size of and weight of your library... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....used so far) or for cancer gene therapy such as nonalcoholic steatohepatitis cancer. In deficiencies of plasma... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....transfection Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines Transformed... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....Spalteholtz (10). He used a solution of benzyl alcohol and methyl salicylate which was later modified ... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....proteins and RNase. Finally, DNA is precipitated with alcohol and resuspended in TE buffer. After this, the DNA... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....enough to dunk your plant’s flowers inside. 50 mL falcon tubes or plastic weigh boats are both great options... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....guide RNA sequence you used. The ICE tool will calculate overall editing efficiency and determine the profiles... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog PostPublishedNov. 15, 2017, 3 p.m....measured by qPCR. Physical titer is typically calculated by two popular PCR-based methods: Quantitative... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m....18845710. Kim, Dal Hyung, et al. "Pan-neuronal calcium imaging with cellular resolution in freely swimming...