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  1. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...I was in luck when I attended a very interesting talk by Prof. Erin M Schuman, a former Caltech researcher...links the azido containing AHA to a fluorescent alkyne tag. The newly synthesized and tagged proteins ...

  2. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...conjugates are Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP). Various substrates for each ... are ready to use off the shelf. So why are we talking about this? Even though there are so many commercially...

  3. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...invitation. 3) Connect with people you have really talked to The best time is within a few days after you...you met IRL (in real life) and perhaps what you talked about (or how good the beer was at that place, ...

  5. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...organize events that bring different voices together to talk about genetics. In addition to events that we put...interface of science and society, and an ability to talk about science in a language that can be understood...

  6. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...before you start. Also, avoid unnecessary talking because talking generates microbe-laden aerosols that may...

  7. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...model. Until then, you can only wonder what I am talking about. How do researchers use physical models of... over and over in their hands. They then start talking to themselves as they compare the features displayed...

  8. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ... our search page (check out this video for a walkthrough of the ways you can search our website). A quicker...about Our New Citations Feature Check out Our Walkthrough of the Deposit Process Learn How We Do Quality...

  9. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...research into the classroom. I had the opportunity to talk with Provost recently about his development of what...Life Sciences Education. 21(4):ar74. Duboue, E., Kowalko, J., Keene, A. et al. 2022. Course-based undergraduate...

  10. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ... cells). The partition NTPase is recruited and “walks” each plasmid to a separate daughter cell. When ...centromere-like region, and the NTPases end up “walking” the same plasmids to one daughter cell, instead...

  12. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...use an orthogonal substrate, alkyne-phenol, to label proteins with an alkyne group for click chemistry reactions...

  13. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...then convince the contacts to give me some time to talk or to at least read my emails. Then pitching myself...meet collaborators  for lunch, make a point of talking to them at a seminars, or even set up regular meetings...

  14. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...? Make an effort to meet people. Attend smaller talks within the bigger conference, especially ones that...that are more career-focused. After these talks, introduce yourself to the speaker and offer your business...

  15. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...involves, and why you might enjoy doing it. I’ll also talk about the skills you’ll need to do well as a healthcare... career in healthcare consulting, I found that talking to alumni, friends, and friends of friends who ...

  16. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...discovered that many of my old suitemates stopped talking to each other if I was not there to arbitrate for... broker a compromise well, you can help everyone walk away from a conflict happy, feeling they each got...

  17. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ... different approaches to be familiar with when talking about IF, direct and indirect. For direct IF, researchers... Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy/   Uhlen M, Bandrowski A...

  18. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...living mouse. Image courtesy of Axel Nimmerjahn (Salk Institute for Biological Studies) Multiple ...light-oxygen-voltage (LOV) domain. They can also be used in crosstalk-free three-color imaging with green and red GFP-like...

  19. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...requirements are similar to standard IF. Here, we’ll walk you through the necessary types of controls for ...., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M., & Weissgerber, T. L. (2021...

  20. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...Pancreas AAV6, AAV8 Wang et al., 2006 Retina 7M8 Dalkara et al., 2013 Skeletal muscle MyoAAV Tabebordbar...1359–1368. https://doi.org/10.1089/hum.2008.123 Dalkara, D., Byrne, L. C., Klimczak, R. R., Visel, M., ...
Showing: 81 - 100 of 256 results