We narrowed to 578 results for: CHI

... starting with an established method called ChIP-seq. ChIP-seq relies on antibodies to recognize and bind...been doing that for years using this assay called ChIP-seq, but it’s really hard for lots of transcription... antibody to any protein to get really beautiful ChIP-Seq results. The FLAG epitope is quite small—20 ...done comparisons of our new system versus standard ChIP-seq for transcription factors known to have good...

...Many thanks to our Guest Blogger Londa Schiebinger. Londa Schiebinger is a leading international expert on... post was contributed by guest blogger Londa Schiebinger, PhD, Hinds Professor of History of Science, ... stem cells. In this case, they found that sex-matching of donor and recipient yielded the best results...the recipient of numerous prizes and awards. Schiebinger addressed the United Nations on the topic of ...

...Guided by dCas9, VP64 recruits transcriptional machinery to specific sequences, causing targeted gene regulation...levels. Although other methods have been able to achieve much higher activation, dCas9-VP64 is great for...of interest, this allows more transcriptional machinery to be recruited per targeted gene. Performance...experiment! References Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable...Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon...

... A., Ichiki, T., Mazmanian, S. K., Osborne, P. B., Keast, J. R., Miller, C. T., Fox, A. S., Chiu, I. M...the brain difficult. Traditionally, delivery is achieved through the use of invasive methods, limiting ...control of transgene expression or activity can be achieved using switches, including riboswitches and optogenetic...Introduction to AAV References Asencor, A. I., Dvoryanchikov, G., Makhoul, V., Tsoulfas, P., & Chaudhari,... W. R., Fish, K. N., & Pfenning, A. R. (2022). Machine learning sequence prioritization for cell type-...j.ymthe.2019.07.017 Matsuzaki, Y., Konno, A., Mochizuki, R., Shinohara, Y., Nitta, K., Okada, Y., & Hirai...Vormstein-Schneider, D., Lin, J. D., Pelkey, K. A., Chittajallu, R., Guo, B., Arias-Garcia, M. A., Allaway, K...

...smaller pieces, and proximity ligation creates chimeric circles of DNA that are distant linearly but close...“picture” is then developed by detecting these chimeric DNA fragments by PCR or next-generation sequencing... outperformed other 3D chromatin methods such as ChIA-PET or Hi-C, by increasing both the number of unique...on-target enrichment. 2. Determining the spatial and hierarchical organization of chromatin interaction networks...CAPTURE 2.0 is the construction of spatial and hierarchical chromatin interaction networks. Think of these...

...partners, and/or children about everything from what you’re going to eat for dinner (frozen chicken fingers or...issue with childcare timing pickup. Whoever takes the earlier slot agrees to pick up both children while the...that the brand new grad student keeps using the machine? Let’s walk through a more specific example....

...Filter By…” options changed when we changed from searching for viral preps instead of plasmids? The available... use to find materials from this publication. Searching for the title of a paper works You can also find...search results to exactly what you need: Better searching with filters Not every plasmid search will start...in the previous layout, the number of plasmids matching each filter is displayed in parentheses.  In this...insert, or use the other options to find plasmids matching your search terms with gene and guide RNA / shRNA...

...they had even established their own labs.  Fried chicken with a side of inspiration  I look back at the ...randomized coffee trials for us at ASCB|EMBO 2019, matching Mentors with Singletons for short coffee breaks... even more valuable program to Microbe 2020 in Chicago.  Finding our place in networking and mentoring...Conference, and the Society for Advancement of Chicanos/Hispanics and Native Americans in Science’s (SACNAS...Joe taught high school chemistry for a year. The teaching/mentoring bug never went away. While he has frequent...

...problem! We also have chimeric versions of Anti-PSD-95 [K28/43R] in rabbit, chicken, and rat. Find Anti-...Tracking cellular history with protein ticker tape Teaching an old antibody new tricks   New compact, efficient... into the TurboID enzyme, Lee and Cheah et al. achieved precise control over when and where the enzyme... bioRxiv — doi: 10.1101/2021.10.13.463862.    Teaching an old antibody new tricks By Ashley Waldron While...

...ophthalmology & visual science 59.3 (2018): 1630-1640. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright... Zebrabow animals to an inducible Cre line. The Schier group developed Zebrabow as a system that takes...optimizes Brainbow for current technologies Tetbow (Sakaguchi et al., 2018) overcame the low expression of XFP...such as the brain, transparent so that the 3D architecture of a fluorescent neuron is visible. Chemical...

...therapeutic settings. Silencing of a target gene is achieved through the delivery of a double-stranded RNA ... plasmids, shRNAs hijack the endogenous miRNA machinery for processing (Figure 1). The shRNA sequence ...the bound miRISC can simply block translation machinery. The composition of the RISC varies, but at the...% end_module_attribute %}{% module_attribute "child_css" is_json="true" %}{% raw %}{}{% endraw %}{% end_module_attribute... Silencing Complex: a versatile gene-silencing machine. Journal of Biological Chemistry, 284(27), 17897...

...has been achieved with the Rinehart system (6); however different proteins will likely achieve different...suppression, as described (6). To determine the stoichiometry of phospho-incorporated to non-phospho-incorporated...-Sung, et al. "Expanding the genetic code of Escherichia coli with phosphoserine." Science 333.6046 (2011...al. "A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation...

...obvious that a desired career outcome is not easily achieved. Of note, it was highlighted during the conference...Positive Points for Trainees - Taking Steps to Achieve a Successful Career Career Advice from the Career...showing researchers that they can take steps to achieve success in their careers. The take-away message...research. As during previous panels, speakers Moon Duchin, Rafael Luna, Joan Reede, Alberto Roca, and Jessica...and stability of academic research. Panelist Moon Duchin provided a concrete example of how underrepresented...

...Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette Knockin...mouse embryos, the embryos allowed to develop into chimeric mice (with a fraction of the cells in the adult...adult mice derived from the engineered ESCs), and chimeric adults mated to produce completely transgenic ...Wang is a professor at the Institute of Zoology, Chinese Academy of Sciences, and he is interested in using...

...dual-nuclease Cas9-Cas9 chimeras Scot Wolfe’s lab has developed dual-nuclease Cas9-Cas9 chimeras with higher target... in Nature Communications Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing...transcription factor localization and binding via ChiP-seq. Learn more about ENCODE Find the transcription...

... Many thanks to our guest blogger, Chinmaya Sandangi! Dr. Chinmaya Sadangi is currently a postdoc at the...This post was contributed by guest blogger Chinmaya Sadangi, a postdoc at the University of Toronto. ...goal of communicating science to non-scientists. Chinmaya Sadangi, a postdoc at the University of Toronto...

...well as archived seminar presentations. One of SITN's newest initiatives, DayCon was the brainchild of SITN...Currently in its 16th year, Science in the News has two chief goals. First, SITN works to bridge the communication...information about SITN: DayCon event website (includes archived content) Science in the News website Check our...

...tumors effectively in mice. Chimeric antigen receptors T cells producing chimeric antigen receptors (CARs)...expressing CD19 chimeric antigen receptors for acute lymphoblastic leukaemia in children and young adults...proteins involved in immune tolerance. Recently, China spearheaded the first ever CRISPR-based clinical...

...wonderful opportunity to once again reflect on our achievements in the last year and share the exciting things...distributor partners, with the launch of antibodies in China and expansion of viral services available in South...partially through work with our distributors in China, Korea, Japan, India, and Brazil. Through an expansion...companies, while making use of our nonprofit status to achieve mission-driven goals of accelerating research and...

...another method Transfection and transduction seem to achieve similar outcomes, so why and when should you opt...Second, what is the biological outcome you want to achieve? For example, do you want the nucleic acid cargo...work is that you can swap out your virus type to achieve different biological outcomes. For example, some...targeting of that virus, which can’t be easily achieved with alternative methods. Ready, set, transduce...