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We narrowed to 509 results for: Cit

Showing: 81 - 100 of 509 results

  1. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...Viral Vectors 101 2nd edition — I was incredibly excited when we picked our viral vector service as one ... is where it’s being delivered. Since target specificity is determined by the capsid of the AAV, many ...approach and involves reading primary literature, soliciting information from requestors and depositors, and...published with a DOI, allowing others to reference and cite the data when deciding on which viral vectors to...to guides to protocols, which was why I was so excited to delve into this data as we updated our Viral...

  2. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...  Lowering cytotoxicity. Different envelope proteins have different levels of cytotoxicity and the mechanisms...mechanisms behind the cytotoxicity can vary. For example, the popular VSV-G envelope protein causes cells... form syncytias that cause the cells to die. Cytotoxicity can also be cell type dependent: if a cell has...connectivity. However, because of rabies virus’s toxicity, it made more sense to pseudotype another virus...

  3. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...extracellular space; they also have low immunogenicity and cellular toxicity. AAV vectors can be equipped with... having the added advantage of lower toxicity, higher specificity and reduced technical variability compared...system where it replicates, spreads, causes neurotoxicity, and is lethal if left untreated (for review...Wang, Y., and Harvey, B.K. (2008). Tropism and toxicity of adeno-associated viral vector serotypes 1,2,5,6,7,8,9...

  4. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...legacy of each government and generation is in the veracity of its work. Alina Chan is a postdoctoral researcher... can follow him on twitter at @thompn4. New York City, NY, USA 40.7128°N 74.0059°W – Deanna McLafferty...that we often find it difficult to convey the excitement and importance of our research to family and ...outside of academia. But the fact is that we are citizens first and scientists second and that it is our...are that we, as a society, need to: 1) use more citations for out data so our sources are readily available...

  5. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...Knocks out a pair of genes pXPR_219 Violet-excited GFP (vex) Rosa26-Cas9 I-CHIME Inducible... knockout of a single gene pXPR_068 Violet-excited GFP (vex) Rosa26-FlpO-ERT2; H11-Cas9 L-CHIME...cell types (lineage-based) pXPR_070 Violet-excited GFP (vex) Lineage-Cre; Rosa26-Cas9 S-CHIME...pairs of genes sequentially pXPR_220 Violet-excited GFP (vex) Rosa26-FlpO-ERT2; H11-Cas9 Table...

  6. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...discoveries, this one was made by accident. After exciting GFP-labeled plant cells at 405 nm instead of 488... GFP could be imaged in the green state after excitation at 488 nm, photoconverted with 405 nm light, ..., and then captured in the red state after excitation at 561 nm. This imaging process worked well with...S65T GFP, and no red color was observed after excitation at 488 nm, indicating that spontaneous conversion...

  7. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ... a quick email at [email protected] with the citation and your deposit or plasmid ID numbers and we’...’ll be happy to update the plasmids to add the citation and release the hold. Increasing impact Making...before publication and we can update the plasmids’ citation when the paper comes out later. Or, if a project... these goals. And we can update your plasmids’ citation to the final journal article version any time ...

  8. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...to a protein of interest thereby giving it the capacity to enter cells. One such CPP was discovered in...protein entry into cells! After verifying the specificity of their luciferase assay, they concluded that...editing. The small molecule NSDB and Na+ hypertonicity are key elements of iTOP. iTOP can be used to... by nature transient, which may lower nuclease toxicity or the potential for off-target effects. Third...

  9. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ...screen, it is necessary to identify the gRNAs that elicit the phenotype, and this depends on the method used...fluorescence changes or a requirement for very high excitation light intensities have made these indicators ...surveyed a range of microbial rhodopsin GEVIs in excitable cells of the nematode Caenorhabditis elegans. ... as the eFRET sensors, QuasAr-mOrange and MacQ-mCitrine. All of these sensors can detect action potentials...

  10. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...you will find our fully-equipped laboratory for citizen scientists. In addition to maintaining a laboratory...over twenty members and volunteers. Transforming citizens into scientists Anyone can become a member of ...microbiome project. This project was inspired by previous city-wide genomic studies and our study will involve ... page on DIYBio.org to find a community lab in a city near you. To learn more about what we’re up to or...

  11. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ... studies And more! Control AAV We’re always excited to add new control AAVs to our inventory since ...biosensing AAV are always being developed and we are excited to offer these useful tools to the community. Among...mCherry 50467  AAV2 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine 121538  AAV5 pOTTC1484 - pAAV SYN1 HA-hM4D(Gi...GlyR IRES EGFP Recombinase AAV Finally, we are excited to add Flp-expression AAV to our recombinase AAV...

  12. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...its mission to make research easier. Addgene is excited to announce that we’ve added John G. Doench, PhD...at like 7 am to go check your plates and you’re excited to do so, you know that that’s the thing for you...on to apply to graduate school at MIT. Doench excitedly remembers: “When I came to MIT to interview, part...miRNAs regulate their targets and what confers specificity in the miRNA regulatory process. This early research...

  13. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...diversity in fluorescence brightness, intensity, excitation and emission spectra. The lab of Dimitri Deheyn...group to perform independent two-color optical excitation of neurons. The paper describes five previously...ShChR, nicknamed Chronos, is blue and green light-excitable and has faster kinetics than those of other channelrhodopsins...method to rapidly address questions of tissue-specificity and cell autonomy of gene function in development...Guo et al., Development. 2014..) A new, higher specificity genome editing system that combines TALENs and... Golden Gate reaction. While the DNA-binding specificity is imparted by the RVD at residues 12 and 13 ...

  14. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...package in low-capacity vectors like AAV, a popular vector due to its low immunogenicity. But these studies...the genome, but Cas13s have higher on-target specificity. New work from Konermann et al. and Yan et al... 2.8 kb in size and are easy to package in low-capacity vectors! These small, but mighty type VI-D enzymes...

  15. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...haven’t been characterized? Some have developed exciting computational algorithms, such as SIFT or PolyPhen...publication We also wrote an extensive review on the exciting new field we call “precision functional genomics....002. We hope that by releasing these new and exciting plasmid collections (together with the data generated...Resources on the Addgene Blog Learn About our Cancer Colleciton Pages Use the ClonTracer Library for Lineage ...

  16. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...Brew BJ, Cunningham AL, Churchill MJ. Pathogenicity and immunogenicity of attenuated, nef-deleted HIV-1 strains... addition to antibody response 2) very high immunogenicity 3) and long-lasting immune response even after... gene therapy. The tractability of AAV, low immunogenicity, and cell-specific targeting features make ...

  17. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...antibodies that are all of the same isotype and specificity. In other words, monoclonal antibodies are derived...also present in other proteins. Because of the specificity of monoclonal antibodies, they also lead to more... - the paratopes - may change and impact the specificity and avidity of the antibodies produced by the...light chain genes within a hybridoma impacts the specificity, avidity, and number of different antibodies ...

  18. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...cells and have an ~8kb packaging capacity. With this packaging capacity, reprogramming factors can be delivered...other delivery methods. Retrovirus has a cloning capacity of ~8 kb, so either a single polycistronic vector...and nondividing cells and have a larger cloning capacity (8-10 kb) than other retroviral vectors. This ...a vector and chromosomal DNA. PB has a cloning capacity of ~9-14 kb. This system is transfected into cells...

  19. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...self-process pre-crRNA into crRNA). The size and simplicity of Cas12a makes it easier to package for therapeutic...therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently been made ...Why would you want to give it a go? The high specificity of Cas14 makes it a promising detection tool ...are 1). non-specific RNA cleavage and 2). cell toxicity. Cas7-11 does not display collateral RNA cleavage...

  20. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...Pharmacology group at Novartis Oncology, is an exciting new tool that allows researchers to individually...barcodes infect the target cell population at low multiplicity of infection (MOI) such that each individual... and sharing protocols one by one,” and she is excited to deposit the library with Addgene because it ...these kinds of reagents.” Here at Addgene, we’re excited to see the creative new ways you put the ClonTracer...
Showing: 81 - 100 of 509 results