...native enzyme, which are summarized in Table 2 below.  Table 2: Properties of native luciferases Organism...ostracod(Cypridina noctiluca)Sea firefly(Cypridina hilgendorfii) 465 62 Cypridina (Vargulin) luciferin None ...

...host range plasmids. FEMS Microbiology Letters, 348(2), 87–96. https://doi.org/10.1111/1574-6968.12241  ...mobilization of broad host-range IncQ plasmids. Plasmid, 62(2), 57–70. https://doi.org/10.1016/j.plasmid.2009.05.001...negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit.22695  Schmidhauser...Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2. Molecular...

...presented in a series of quick (10 minute) or very quick (2 minute) talks so we got to hear from everyone. Addgene...evolutionary tree at least 6-7 times and on at least 2 separate continents. Mimulus (Andrea Sweigart) Monkeyflowers...Organisms, Part 1 Five Popular Model Organisms, Part 2 ...lab has the capability) with receptor mediated endocytosis. They used this to edited those brown marmorated...

...to a specific area at the plasma membrane (Figure 2). Just express the plasma membrane-localized iLID ...cells will be ready for the spotlight.    Figure 2: Opto-RhoGEFs to control Rho GTPase activity. A) Schematic...OptoTIAM (magenta) expressed in a monolayer of endothelial cells, showing cell protrusions and overlaps ...junction integrity in the vascular barrier of the endothelium, but they could be applied to study other actin-dependent...provided CIB constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD...scale, enabling precise control over vascular endothelial barrier strength. eLife, 12, RP84364. https:/...

...expressed as a fusion with your favorite protein and (2) a synthetic fluorophore-contianing “ligand” that ...cell 58.4 (2015): 644-659. PubMed PMID: 26000849. 2. Grimm, Jonathan B., et al. "A general method to improve...conjugated to molecular species with high affinity for endogenous molecular targets such as paclitaxel. Unlike ...required as the small-molecule binding motif targets endogenous protein (Figure 1f). Labeling strategy pros...

...green squares, purple circles and blue triangles. 2) An antibody immobilized on a bead binds specifically...protein G, a novel IgG-binding reagent. J Immunol 133(2):969-974. Hjelm H, Hjelm K, Sjöquist J (1972) Protein...typically expressed at much higher levels than endogenous proteins. Data obtained from experiments using...tagged protein may not be translatable to the endogenous system and will need to be verified using alternative...

...is always best to have options.      Fig. 2: Addgene’s western blot for mEGFP-HRas (from Plasmid...the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods   Find YFP plasmids...also demonstrated the ability to be used as an endogenous RNA imaging tool.   Fig. 1: Csm, shRNA...

... as muscular dystrophies, heart disease, and type 2 diabetes, across four different types of human cells...(#186715) and hCtRNA_VT (#186716).   Fig. 2: Comparison of previous representative dual-gRNA architectures...expression, the individual gRNAs are released by endogenous tRNA processing machineries, specifically, RNaseZ...

...while increasing the standard mutation rate by only ~2-fold over nCas9 levels. Two sets of modifications ...10-3 mutations per nucleotide per generation, and (2) the addition of the thioredoxin-binding domain (TBD...extends it from the 3’ end, while its native endonuclease activity degrades the displaced strand.   ...

... is the genetic test used for sickle cell anemia (2). Southern blotting, in conjunction with restriction...program) can then reproduce the DNA sequence (Figure 2). Accuracy and accessibility led to the widespread...biology98.3 (1975): 503-517. PubMed PMID: 1195397. 2. Chang, Judy C., and Yuet Wai Kan. "A sensitive new...cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae." Proceedings of the...

...Table 2: Lab strains of E. coli Strain Natural resistance Primary Use Genotype ccdB Survival 2 T1R ...resistance to tetracycline Additionally, Table 2 provides a quick reference for some of the popular... of certain expressed proteins endA, endA1 Endonuclease I (nonspecific cleavage of dsDNA ) mutation ...

... tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain. PLoS One. 2013 ...PMID: 24086725. PubMed Central PMCID: PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher...recommends using an anti-mCherry antibody rather than endogenous mCherry fluorescence when visualizing hM4D(Gi...

...VP64 fusion; 2. A sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop 2; 3. The MS2...complex for the transcriptional activation of endogenous genes and it consists of three components: 1....

... to share information about the emerging SARS-CoV-2 virus quickly and openly. By sharing results faster...Open Scientific Communication Ecosystem Fig. 2: The scientific ecosystem, enriched by open access...providing a public record of changes, critiques or endorsements. In contrast, journal-based peer review is usually...

...First, the light-responsive molecules of cryptochrome 2 are the chromophores flavin-adenine dinucleotide and...it as “a novel mode of optogenetic control of endogenous cellular processes." The LITE system can now ...Konermann et al. "Optical control of mammalian endogenous transcription and epigenetic states." Nature....

...faster when using a dual guide system.   Fig. 2: Size screening for large deletion events.   ... Biol 17: https://doi.org/10.1186/s13059-016-1012-2 Find more CRISPR Resources at Addgene: Read our CRISPRGuide...this method, but your edit doesn’t affect any endogenous restriction enzyme sites? Never fear! You can...Resources References Eva K. Brinkman, Tao Chen, Mario Amendola, Bas van Steensel. Easy quantitative assessment...

...plasmid kit consists of 20 plasmids encoding 14 alpha, 2 beta, and 4 gamma subunits of the heterotrimeric G...activation via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on GPCR signaling...chemogenetics as they are relatively insensitive to their endogenous ligand, acetylcholine, and are only activated...

..., et. al. Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell, 163(3): 759-771...11 and Cas13 are 1). non-specific RNA cleavage and 2). cell toxicity. Cas7-11 does not display collateral...

... strategy for linking RSM inputs to endogenous gene expression (2). Our paper (1) explains how to arrange...certain states of interest. For example, in Figure 2 below we present a two-input, five-state GRSM register...Science. 353, aad8559 (2016). PubMed PMID: 27463678. 2. Green, Alexander A., et al. "Toehold switches: de-novo-designed...

...without leaving behind any accessory DNA sequences [2], [3]. Simply put, the piggyBac™ transposon containing...with a Footprint-Free™ genome edit.   Figure 2: Editing DNA using the piggyBac™ transposon selection...PMID: 26211958. PubMed Central PMCID: PMC4663986. 2.  A. M. Singh, V. V Adjan Steffey, T. Yeshi, and D...genome following donor removal, which alters the endogenous DNA sequence and has been suggested to cause ...