...expression, measured using fluorescent protein fusions In addition to visible light, flow cytometers can...Which Fluorescent Protein Should I Use? However, fusion proteins tag your protein of interest with a fluorescent...- or C-terminus of the protein. One downside of fusion proteins is that the tag can alter the structure...get around the challenges of fluorescent protein fusions by binding to the protein in its native state.  ...

... any tags or fusions that are on your insert. Typically you would list any tag or fusion protein in the... pBACKBONE-Flag-hGene If there was also an EGFP fused to the C-terminal of your insert you would list ...

...After testing dCas9 fusions with transcriptional repressors, they found that fusing dCas9 to an Mxi1 repressor...covalently attached to the Cas9-gRNA RNP complex via the fused HUH endonuclease PCV. This increases HDR efficiency...

..., Georgieva et al. examined the effects of GFP fusions to inntags or traditional FLAG, HA, and Myc tags...untagged GFP overexpression, while the rest of the fusions had some negative effect on these parameters. IT5... cell morphology was observed with IT5 and IT6 fusions. In contrast, Flag and HA tags had especially detrimental...

...from the Zhang Lab. It's name, though a little confusing to the uninitiated, contains much of the information...directed by a gRNA. 2A-Puro In this plasmid, SpCas9 is fused to the puromycin resistance gene through a 2A peptide...the 2A peptide on its C-terminus, SpCas9 is also fused to a 3X FLAG tag at its N-terminus. This tag can...

...they identified included gene amplifications and fusions, missense mutations, and indels (Ng et al., 2018...carrying a single gene with a defined mutation or gene fusion. Plasmids carrying the corresponding wild-type ...Mutations in Cancer.” Of all the mutations and fusions represented in this collection, approximately 80%...

...genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays in the cytoplasm...vector to create knock-ins of fluorescent proteins, fusion proteins, or transcriptional regulators. In this...

... for Goldengate cloning   Design primers for In-Fusion Cloning Design primers for Gibson Assembly and ...restriction enzyme cloning, Gibson assembly, or In-Fusion cloning.  NEB Ligation Calculator: This tool works... from five different insert:backbone ratios. In-Fusion cloning molar ratio calculators Lab math calculators...

...an exon of a gene to generate a transcriptional fusion. This may disrupt the endogenous function of the...the gene product resulting in a transcriptional fusion, much like promoter traps. While this system does...disrupt the gene it monitors due to transcriptional fusion.   Examples of promoter, enhancer, and gene...

...by using the DNA cutting activity of a Protein A fused micrococcal nuclease (MNase) to specifically isolate... against the protein of interest. The Protein A fused MNase is then added and Protein A binds Immunoglobulin...protein interactions Learn about other types of fusion proteins Study chromatin interactions with CAPTURE...

...biosensors contain a single fluorescent protein fused to a domain that senses the concentration of a given...an example. Many calcium biosensors include GFP fused to calmodulin (CaM) and the chicken myosin kinase... an intracellular or extracellular molecule? By fusing a biosensor to an organelle-specific targeting ...

...available – you can always add an epitope tag or a fusion protein. Mammalian vectors One of our most popular... cells. The addition of an in-frame fluorescent fusion protein will allow you to visualize YGOI in vivo... offer a collection of vectors carrying several fusion proteins. Pick your promoter, selection cassette...

...sites to tether many copies of the FP-coat protein fusions to their reporter mRNAs. Using translation inhibitors...to the plasma membrane; this sequence prevents diffusion of the mCherry labeled mRNA and keeps it in a ...SunTag, a synthetic scaffold that can recruit GFP fused to the SunTag-specific antibody scFv. Each time ...

...conditionally and reversibly degrade proteins of interest fused to a degron, a domain that induces degradation. ...In addition to the engineered SpCas9, they also fused the SpCas9-NG with the activation-induced cytidine...light-activated-dynamic-looping (LADL) system. The group fused dCas9 to a truncated CIB1 protein from Arabidopsis...

...binding protein known as GltI. When the resulting fusion protein binds to glutamate, conformational changes...original iGluSnFR in the pMinDisplay backbone is fused to a specific peptide segment (PDGFR TMD) that anchors...Aggarwal et al… Figure 4. PDGFR is a C-terminal fusion to the PDGFR transmembrane domain in the mammalian...

...diversification by targeting a site of interest with dCas9 fused to the Activation Induced Cytidine Deaminase (AID...two key components: the error prone PolI3M that’s fused to a nicking Cas9 (nCas9). Similar to mutagenesis...consists of an error-prone polymerase (PolI) that is fused to a nicking Cas9. A guide directs Cas9 to a site...

... mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag fusions, while the anti-HA frankenbodies include mEGFP,...Leeson The large size of CRISPR-Cas effectors and fusion proteins, such as Cas9 and Cas12a, creates challenges...from Xiaoshu, et. al. 2021.   Created by the fusion of Cas12f with a transcriptional activator, CasMINI...

...producing monoclonal antibodies is from a hybridoma, a fusion between an antibody-producing B cell and a myeloma...cells from an immunized animal, those B cells are fused to myeloma cells at a 1:1 ratio and then cultured...medium called HAT. This medium selects against unfused myeloma cells and antibody-producing B cells that...

...beta-arrestin-TEV protease fusion to a receptor-transcription factor fusion. TEV protease cleave the transcription... et al., 2017). In this technique, the receptor fused to APEX2 catalyzes the biotinylation of proteins...

...interference (CRISPRi) targets a catalytically dead Cas9 fused to a transcriptional repressor to your gene of interest...activators (CRISPRa) are transcriptional activators fused to a dead Cas9. The system is led, via a custom ...specific ligand to mark the tag and whatever it is fused to for degradation. Since the tagged protein of ...