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We narrowed to 388 results for: HAL;

Showing: 81 - 100 of 388 results

  1. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...hematopoietic disorders sickle cell anemia and beta-thalassemia. Lentiviral vectors are promising agents for ...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. ...HMGA2 activation after gene therapy of human β-thalassaemia. Cavazzana-Calvo M, Payen E, Negre O, Wang G...

  2. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...gRNA together (~4.2 kb) into an AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb...transgenes might seem daunting, it’s actually a challenge that the field has overcome with split AAVs. In...WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang...

  3. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...RNA is not inherently fluorescent and can be challenging to track intracellularly, but aptamers permit... when bound to DFHBI despite being approximately half the length (Warner et al., 2014); its length reduces...next additions to aptamer soup! References Alam, Khalid K., et al. "A fluorescent split aptamer for visualizing...

  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... et al., Science. 2014. Jan 3;343(6166):80-4. Shalem et al., Science 2014. Jan 3;343(6166):84-7. Koike-Yusa...identified a family of GFP proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They...implicated in disease progression. One of the major challenges in signaling research is the inability to replicate... (including His, S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for optimizing expression, ...

  5. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...become bored because I was not being mentally challenged. For these and other reasons, I started  looking...combination of genetic mutations. This is a particular challenge in pancreatic cancer where one drug does not work...while I’m based in London) does bring its own challenges. I’ve had to become skilled with technology like...

  6. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...That’s what makes it so wonderful. The most challenging part of jumping into this career path from academia... your hair out, your successes, failures, and challenges you’ve overcome. This information may help you...completely new field without issue. This may be more challenging if you are trying to apply for an outright job...

  7. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...the University of California Berkeley. It’s been half a year since the march for science on April 22, ...present at the lab bench," Liu, a UM undergraduate, challenged us. I worried the march would be a simple, unquestioning... fingers of skepticism to defend an agenda that halts current data collection, destroys existing data,...

  8. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ... lymph nodes at different times after antigen challenge, scientists were able to track where GFP labelled...et al. 2016, Cavanagh et al. 2008)? Technical challenges of two photon microscopy Intravital imaging is... at steady state or under physio-pathological challenge. However technical specificities have to be taken...

  9. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...that code for surface proteins. One of the first challenges with a custom screen approach is to identify ...PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide...essentiality profiling reveals gene networks and synthetic lethal interactions with oncogenic Ras." Cell 168.5 (2017...

  10. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ... This is the first half of a two-part interview with Vini Mani and Amy Gilson from Science in the News...eager to communicate their science. In this first half of our two-part interview with Vini and Amy, we’...

  12. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    Published
    July 1, 2014, 3:42 p.m.
    ... about salary, opportunities for advancement, challenges and disappointments. These more personal questions...people honor. Talking with strangers is usually challenging but does get easier with practice. Another perk...

  13. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...This is the second half of a two-part interview with Vini Mani and Amy Gilson from Science in the News...involved in science communication. In this second half of our conversation with Vini Mani and Amy Gilson...

  14. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ... cancer immunotherapy targets but are embryonic lethal, hindering studies post embryogenesis. Using C-... in a developing or developed immune system was lethal (LaFleur et al., 2024).   Figure 1: Schematic...

  15. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...that Addgene employees created and designed for the Hall of Human Life at the MOS. (If you want a fun refresher... ghost writers, of course! Amy Uddin and Emma Gottschall, two of Addgene's lab technicians, gave Blugene...

  20. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...problem that he considers “one of the greatest challenges of neuroscience.”  Hot plasmids: Twitch sensors...trial and error. Transition metals also present challenges in that they are found in cells at much lower...
Showing: 81 - 100 of 388 results