... et al., Science. 2014. Jan 3;343(6166):80-4. Shalem et al., Science 2014. Jan 3;343(6166):84-7. Koike-Yusa...identified a family of GFP proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They...implicated in disease progression. One of the major challenges in signaling research is the inability to replicate... (including His, S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for optimizing expression, ...

...become bored because I was not being mentally challenged. For these and other reasons, I started  looking...combination of genetic mutations. This is a particular challenge in pancreatic cancer where one drug does not work...while I’m based in London) does bring its own challenges. I’ve had to become skilled with technology like...

...That’s what makes it so wonderful. The most challenging part of jumping into this career path from academia... your hair out, your successes, failures, and challenges you’ve overcome. This information may help you...completely new field without issue. This may be more challenging if you are trying to apply for an outright job...

...the University of California Berkeley. It’s been half a year since the march for science on April 22, ...present at the lab bench," Liu, a UM undergraduate, challenged us. I worried the march would be a simple, unquestioning... fingers of skepticism to defend an agenda that halts current data collection, destroys existing data,...

... lymph nodes at different times after antigen challenge, scientists were able to track where GFP labelled...et al. 2016, Cavanagh et al. 2008)? Technical challenges of two photon microscopy Intravital imaging is... at steady state or under physio-pathological challenge. However technical specificities have to be taken...

...that code for surface proteins. One of the first challenges with a custom screen approach is to identify ...PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide...essentiality profiling reveals gene networks and synthetic lethal interactions with oncogenic Ras." Cell 168.5 (2017...

... This is the first half of a two-part interview with Vini Mani and Amy Gilson from Science in the News...eager to communicate their science. In this first half of our two-part interview with Vini and Amy, we’...

...kinase activity is FRET, but FRET reporters are challenging to design and difficult to multiplex. The Covert...which fluorophores can be multiplexed together. Challenging  to Design: FRET reporter activity depends on...

... about salary, opportunities for advancement, challenges and disappointments. These more personal questions...people honor. Talking with strangers is usually challenging but does get easier with practice. Another perk...

... cancer immunotherapy targets but are embryonic lethal, hindering studies post embryogenesis. Using C-... in a developing or developed immune system was lethal (LaFleur et al., 2024).   Figure 1: Schematic...

...This is the second half of a two-part interview with Vini Mani and Amy Gilson from Science in the News...involved in science communication. In this second half of our conversation with Vini Mani and Amy Gilson...

...that Addgene employees created and designed for the Hall of Human Life at the MOS. (If you want a fun refresher... ghost writers, of course! Amy Uddin and Emma Gottschall, two of Addgene's lab technicians, gave Blugene...

...systems, modifications to the RNA components, and challenging target genomes, such as the malaria-causing Plasmodium...Addgene depositor, discussed some of the many challenges of creating biological circuits, and I can only...

...one of two global stock centers for Arabidopsis thaliana (Arabidopsis). Our mission is to collect, preserve...system comes with fewer ethical and logistical challenges than does working with animals. Because Arabidopsis...

...staining can be a challenge in the best of cases. But it can be particularly challenging when your only ...

...Schiebinger, another Quake Lab member joined for half of the trip. Preparations ahead of time included...spring requiring an overnight hike in. Navigating challenges: collecting and processing environmental samples...

...problem that he considers “one of the greatest challenges of neuroscience.”  Hot plasmids: Twitch sensors...trial and error. Transition metals also present challenges in that they are found in cells at much lower...

...Associate Professor at University of Barcelona. The challenge: Making courses on genetic manipulation more hands...could be played when and, depending upon who your challenger was, you might have to drastically alter your...

...exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for all ages...environment.  Be kind to yourself. This is a challenging period for everyone for numerous reasons, and...

...these indicators difficult to work with.  The Gottschalk lab has surveyed a range of microbial rhodopsin...previously reported for other organisms, and the Gottschalk group hypothesizes that this may be due to different...