We narrowed to 129 results for: JUN

1. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   ...Myers of HudsonAlpha (where Mendenhall is also an adjunct faculty member) have together been working toward...

2. Transferable Skills Guide: Cross-team Communication

   Type

   Blog Post

   Published

   Nov. 21, 2019, 1:53 p.m.

   ...collaborative lab work. Corralling context and junking jargon I suspected I knew at least one source of...

3. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   Published

   June 15, 2017, 2:30 p.m.

   ...fusions to connexin localized correctly to gap junctions (Shaner et al, 2008). Obligate dimers (such as...

4. Addgene: The First Twenty Years

   Type

   Blog Post

   Published

   Dec. 19, 2024, 2:15 p.m.

   ...depositors whose plasmids we were entrusted with. So in June 2016, we launched the Blue Flame Award, which recognizes...

5. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   Published

   May 30, 2023, 1:15 p.m.

   ... 6.2 (2002): 272. PubMed PMID: 12349826. Xiong, Wenjun, and Constance Cepko. "Distinct expression patterns...

6. Addgene's Tips for Plasmid Quality Control

   Type

   Blog Post

   Published

   Jan. 14, 2016, 3:30 p.m.

   ...predecessor. Important features may include cloning junctions (where most sequence assembly errors occur), deletions...

7. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   Published

   Oct. 20, 2015, 2:30 p.m.

   ...Shaping the Future of Research: a perspective from junior scientists." F1000Research 3 (2014). Pubmed PMID...

8. Plasmids 101: Cre-lox

   Type

   Blog Post

   Published

   Jan. 13, 2015, 3:47 p.m.

   ...alternative to Cre-lox, and has also been used in conjunction with it, allowing for two separate recombination...

9. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

   Type

   Blog Post

   Published

   Aug. 20, 2019, 12:31 p.m.

   ... vectors Behavioral testing is often done in conjunction with a genetic manipulation so that behavior ...

10. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...CRISPR Guide to Epigenetics References Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3...

11. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ...Kapanidis, Achillefs N. "Taking the ruler to the jungle: Single-molecule FRET for understanding biomolecular...

12. Hot Plasmids February 2024

    Type

    Blog Post

    Published

    Feb. 20, 2024, 2:45 p.m.

    ...cellular entry with other transfection methods. The Junwei Shi Lab addressed this problem by optimizing the...

13. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type

    Blog Post

    Published

    Sept. 13, 2016, 2:30 p.m.

    ...Shaping the Future of Research: a perspective from junior scientists."F1000Research 3 (2014). Pubmed PMID...

14. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...improving my skills and sharing with other more junior leaders what I had experienced and learnt. Opening...

15. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    Published

    June 25, 2024, 1:15 p.m.

    ... S., Min, S., Bae, J. Y., Choi, J. W., Park, J., Jung, D., Yoon, S., & Kim, H. H. (2019). SpCas9 activity...

16. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    Published

    Feb. 23, 2016, 3:30 p.m.

    ...clones displayed proper insertion at the 5’ and 3’ junctions, respectively. These results indicate that PITCh...

17. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    Published

    Sept. 24, 2015, 2:30 p.m.

    ...while a catalytically dead dCas9 can be used in conjunction with activator or repressor domains to control...

18. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    Published

    Aug. 27, 2020, 1:15 p.m.

    ...pdb.prot076497. https://doi.org/10.1101/pdb.prot076497 Kim Y, Jung JP, Pack C-G, Huh W-K (2018) Global analysis of ...

19. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    Published

    July 16, 2020, 1:15 p.m.

    ...has had a tremendous impact on our world -- as of June 4, 2020, COVID-19 has caused an estimated total ...

20. MXS Chaining

    Type

    Blog Post

    Published

    Feb. 7, 2017, 3:30 p.m.

    ...acids. The Journal of biological chemistry. 1962. June;237:1961–1967. Pubmed PMID: 13918810. ...