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We narrowed to 86 results for: K-Ras protein

Showing: 81 - 86 of 86 results
  1. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...the same page in terms of the vocabulary--DNA, protein, plasmid, gene, etc. Though I talked about CRISPR...teach  5-10 year olds in a single evening. Grades K-5 cover a broad range of attention spans, skill levels... I came up with an analogy that would help them grasp some of the key concepts (Fig. 2). One thing I will...
  2. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... be used as a proxy measure for differences in protein expression. (If you're just getting started, check... amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma, 2019; Trampuz, 2003.   You...doi.org/10.1038/s41598-017-02217-xTaylor, SC; Nadeau, K; Abbasi, M; Lachance, C; Nguyen, M; Fenrich, J. The...References and Resources  References Biassoni R; Raso A. (eds.), Quantitative Real-Time PCR: Methods and...
  3. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...components including (1) a CRISPR RNA, (2) four proteins forming the QCascade DNA-targeting module with...with the crRNA, (3) three transposase proteins, and (4) the donor DNA. By using a multi-spacer CRISPR array...Zalatan’s lab used an approach to identify known proteins involved in the activation of transcription including...including transcription factors, phage proteins, and RNAP components (Dong et al., 2018). They found a...dCas9 variant fused to bacterial enhancer binding protein required for the activation of sigma-54 type promoters...glycosylase inhibitor and degradation tag on the fusion protein to limit the activity of the system as a whole.... Rep 6: . https://doi.org/10.1038/srep19452 Selle K, Fletcher JR, Tuson H, Schmitt DS, McMillan L, Vridhambal...
  4. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...complexes to study neural function. Often the reporter proteins associated with these complexes are so robustly...vectors: packaging capacity and the role of rep proteins in vector purity." Human gene therapy 18.2 (2007... 1248. PubMed PMID: 11509958. 8. Ferrari, Forrest K., et al. "Second-strand synthesis is a rate-limiting...Surprisingly, little research has compared and contrasted optogenetic and chemogenetic receptors delivered...
  5. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ..., Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht...exciting for scientists working to visualize multiprotein complexes.  Fig. 2. Multiplex immunolabeling...
  6. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...cancer.     References 1. Russell, David W., and Roll K. Hirata. "Human gene targeting by viral vectors." ...devised methods to deliver CRISPR components as ribonucleoproteins (RNPs) that can be supplied separately from.... PubMed Central PMCID: PMC4752446. 5. Asuri, Prashanth, et al. "Directed evolution of adeno-associated...28646206. PubMed Central PMCID: PMC5482879. 7. Bak, Rasmus O., and Matthew H. Porteus. "CRISPR-mediated integration...
Showing: 81 - 86 of 86 results