We narrowed to 163 results for: Kars

1. Fluorescent Proteins 101: Fluorescent Biosensors

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   Blog Post

   Published

   May 18, 2017, 2:30 p.m.

   ...your biosensor Before expressing a biosensor in eukaryotic cells, think about the best place for the biosensor...

2. SunTag and Fluorescent Imaging

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   Blog Post

   Published

   March 28, 2017, 2:30 p.m.

   ... your imaging time to get enough signal. This workaround risks cellular phototoxicity and eliminates the...

3. Plasmids 101: Antibiotic Resistance Genes

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   Blog Post

   Published

   Jan. 30, 2014, 3:29 p.m.

   ...translocation Bacteriostatic 5-25 ug/mL in EtOH *In prokaryotes.     **Dissolve in dH2O and sterile filter unless...

4. Meet the New Addgene Board Members!

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   Blog Post

   Published

   April 28, 2020, 1:14 p.m.

   ... and former Associate Director of Viral Vectors Karen Guerin for the journal Molecular Therapy. Vandenberge...

5. New and Upcoming Viral Vectors - December 2019

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   Blog Post

   Published

   Dec. 19, 2019, 2:40 p.m.

   ...We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon...

6. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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   Blog Post

   Published

   April 25, 2019, 3:01 p.m.

   ...State University, Indiana University, and the Karolinska Institute developed mNeonGreen via 21 substitutions...

7. Hot Plasmids and Viral Preps - September 2021

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   Blog Post

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   Sept. 21, 2021, 1:15 p.m.

   ...previously developed such a system in Streptomyces, a prokaryotic genus capable of producing a variety of natural...

8. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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   Blog Post

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   June 22, 2021, 1:15 p.m.

   ..., Giandhari J, Pillay S, Wilkinson E, Naidoo Y, Karim F, Ganga Y, Khan K, Bernstein M, Balazs AB, Gosnell...

9. Plasmids 101: Origin of Replication

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   Blog Post

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   Nov. 11, 2020, 2:23 p.m.

   ...simplicity’s sake, we've ignored those used in eukaryotic cells and viruses and focused only on those found...

10. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    Blog Post

    Published

    Aug. 18, 2023, 4:15 p.m.

    ...infected cell.) This is in contrast to bacteria and eukaryotes, which use a binary replication process whereby...

11. Another Pathway into Cells: iTOP

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    Blog Post

    Published

    June 23, 2015, 8:37 p.m.

    ...Native Proteins. D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V, Lebbink RJ, Rehmann H...

12. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    Blog Post

    Published

    April 7, 2015, 4:21 p.m.

    ...of multiple loci is deftly coordinated by the eukaryotic cell for the execution of many complex cellular...

13. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    Blog Post

    Published

    Sept. 3, 2019, 1:15 p.m.

    ...sequence and ultimately its degradation. While other eukaryotes don’t naturally have this response, they do contain...

14. "Build Your Plasmid": The Game - Play to Teach

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    Blog Post

    Published

    July 17, 2018, 12:23 p.m.

    ... students are not even allowed to manipulate prokaryotic cells? Especially, if you only have 4 hours and...

15. Plasmids 101: Mammalian Vectors

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    Blog Post

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    March 25, 2014, 3:15 p.m.

    ... 100-400 ug/mL *Not comprehensive.   ** In eukaryotes.   ***The concentration used for selection is...

16. Antibodies 101: Monoclonal Antibodies

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    Blog Post

    Published

    June 29, 2021, 1:15 p.m.

    ...https://doi.org/10.1093/ilar.46.3.258  Mould AP, Askari JA, Byron A, Takada Y, Jowitt TA, Humphries MJ ...

17. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post

    Published

    May 9, 2017, 2:12 p.m.

    ... Gootenberg, J., Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015). Cpf1...

18. Addgene’s Viral Service - Why Virus? Why Now?

    Type

    Blog Post

    Published

    March 23, 2017, 2:30 p.m.

    ... our virus. Ed Boyden’s lab from MIT and Alla Karpova’s lab from Janelia Research Campus have provided...

19. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    Published

    Jan. 31, 2019, 1:32 p.m.

    ...PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine ...

20. Antibodies 101: Affinity Reagents

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    Blog Post

    Published

    April 14, 2022, 1:15 p.m.

    ...monobodies can be easily expressed in transfected eukaryotic cells, allowing for them to be used as intracellular...