...reprobing works best with western blots that use chemiluminescent or fluorescent detection, like horseradish...remains, whether that involves adding fresh chemiluminescent substrate or imaging your fluorescent probe...

... expected range. For example, calcium in the ER lumen reaches the sub-millimolar range, much higher than...state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure to increased...

...studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually introduced...1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https://doi.org/10.1016...

... of the curve, avoid pipetting small volumes (< 2 µL) or volumes too large for a standard micropipette...

...Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical Center. DNA transposons are...currently a PhD candidate in the Sternberg Lab at Columbia University Medical Center. He currently studies...

...= {[(R*C)(1000/V)]*D} R = Reaction volume C = Copies/uL V = Volume of virus in reaction mix D = Dilution...

...metal-affinity chromatography, usually using a Ni2+ column. Like other affinity tags, a His tag can be fused...with the cleavage reaction taking place between column runs. The TEV protease itself, with various mutations...

...variety of chromogenic, chemifluorescent, and chemiluminescent substrates are available. Labs with limited...reader as opposed to chemifluorescent, and chemiluminescent methods that require specialized equipment...

...dark areas (protected) as opposed to brightly illuminated ones (risky). They also prefer to explore novel...References Alpár, Alán, et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability...

...spp., Aliivibrio salmonicida, Anabaena spp., Azospirillum braziliense, Azotobacter spp., Bordetella spp...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...

...Master. Note: BRET= Bioluminescence Resonance Energy Transfer CRET= Chemiluminescence Resonance Energy Transfer...

... 37°C heat block, LED blue light illuminator or UV light illuminator. Approach: Highly similar to the ...

...qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini et al., 2012) measures...homodimerization of FPs targeted to the endoplasmid reticulum based on the appearance of OSER whorls. This assay...

...sensitivity, and photostability under two-photon illumination than previous GEVIs. Some constructs are also...00916-3. doi: 10.1016/j.cell.2022.07.013 pYtags illuminate RTK signaling Receptor tyrosine kinases (RTKs...

...trace decomposition. (2014) Nucleic Acids Research Volume 42, Issue 22, Page e168. https://doi.org/10.1093...CRISPR-Cas9 editing. (2018) Nucleic Acids Research Volume 46, Issue 10, Page e58. https://doi.org/10.1093...

...primer to each template. The PCR product is then column purified. Degrade wild-type template strand: Next...23766324. PubMed Central PMCID: PMC4409680. 2. Mccullum, E. O., Williams, B. A., Zhang, J., & Chaput, ...

... sequencing, (which differ from the short-read Illumina MiSeq sequencing that Addgene uses.) However, ...article.   Long-read sequencing Addgene uses Illumina MiSeq NGS to perform whole plasmid sequencing ...

...recently developed a new method called DULIP (dual luminescence-based co-immunoprecipitation) to overcome the... this kit are derived from the Ralser lab's own pHLUM minichromosomal vector, and besides being used for... pooled library Parasites from the apicomplexan phylum cause severe human and livestock diseases such ...

...so don’t be shy and reach out! Someone from your alumni community is a great place to start. What is science.... Many fellowship programs can connect you with alumni before and during your application process so you...

...PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent assay for detection of extremely rare mutations... probe DNA-immobilized magnetic beads and chemiluminescence detection." Analytical Sciences 29.7 (2013...