Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 213 results for: LUM;

Showing: 81 - 100 of 213 results

  1. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...reprobing works best with western blots that use chemiluminescent or fluorescent detection, like horseradish...remains, whether that involves adding fresh chemiluminescent substrate or imaging your fluorescent probe...

  2. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... expected range. For example, calcium in the ER lumen reaches the sub-millimolar range, much higher than...state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure to increased...

  3. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually introduced...1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https://doi.org/10.1016...

  5. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical Center. DNA transposons are...currently a PhD candidate in the Sternberg Lab at Columbia University Medical Center. He currently studies...

  6. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...= {[(R*C)(1000/V)]*D} R = Reaction volume C = Copies/uL V = Volume of virus in reaction mix D = Dilution...

  7. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...metal-affinity chromatography, usually using a Ni2+ column. Like other affinity tags, a His tag can be fused...with the cleavage reaction taking place between column runs. The TEV protease itself, with various mutations...

  8. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...variety of chromogenic, chemifluorescent, and chemiluminescent substrates are available. Labs with limited...reader as opposed to chemifluorescent, and chemiluminescent methods that require specialized equipment...

  9. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...spp., Aliivibrio salmonicida, Anabaena spp., Azospirillum braziliense, Azotobacter spp., Bordetella spp...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...

  10. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...dark areas (protected) as opposed to brightly illuminated ones (risky). They also prefer to explore novel...References Alpár, Alán, et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability...

  11. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...Master. Note: BRET= Bioluminescence Resonance Energy Transfer CRET= Chemiluminescence Resonance Energy Transfer...

  12. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...sensitivity, and photostability under two-photon illumination than previous GEVIs. Some constructs are also...00916-3. doi: 10.1016/j.cell.2022.07.013 pYtags illuminate RTK signaling Receptor tyrosine kinases (RTKs...

  13. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...trace decomposition. (2014) Nucleic Acids Research Volume 42, Issue 22, Page e168. https://doi.org/10.1093...CRISPR-Cas9 editing. (2018) Nucleic Acids Research Volume 46, Issue 10, Page e58. https://doi.org/10.1093...

  16. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...primer to each template. The PCR product is then column purified. Degrade wild-type template strand: Next...23766324. PubMed Central PMCID: PMC4409680. 2. Mccullum, E. O., Williams, B. A., Zhang, J., & Chaput, ...

  17. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ... sequencing, (which differ from the short-read Illumina MiSeq sequencing that Addgene uses.) However, ...article.   Long-read sequencing Addgene uses Illumina MiSeq NGS to perform whole plasmid sequencing ...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...recently developed a new method called DULIP (dual luminescence-based co-immunoprecipitation) to overcome the... this kit are derived from the Ralser lab's own pHLUM minichromosomal vector, and besides being used for... pooled library Parasites from the apicomplexan phylum cause severe human and livestock diseases such ...

  19. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...(Erdmann et al., 2019). Check out our recent Illuminating Choices blog post for more discussion about ...from Addgene Better Dyeing Through Chemistry Illuminating Choices: A Guide to Selecting Fluorescent Dyes...

  20. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent assay for detection of extremely rare mutations... probe DNA-immobilized magnetic beads and chemiluminescence detection." Analytical Sciences 29.7 (2013...
Showing: 81 - 100 of 213 results