...exchange vector. This vector contains sacB, an chloramphenicol resistance cassette, and the R6K origin of ...products. To allow manipulation of naturally chloramphenicol-resistant strains, we have also included versions...exchange system, he is so excited to use it to explore how hospital-associated Gram-negative bacteria ...

...targets with different colors. Multiplex immunofluorescence Speaking of different colors, having a palette ...secondary antibodies in a few different species and colors makes it easy to perform multiplexed IF. By using...

...limited selection of fluorophores. If the available colors are already reserved for other markers in your ... all cells positively labeled with the selected color will not be CD8+ T cells and we can simply “dump...undesired cell populations. Dump gating uses the same color for different markers, making it easy to gate on...

...with many challenges, researchers have begun to explore genome editing approaches for exon skipping. Addgene...diseases will require HDR-mediated precision editing tailored to smaller patient populations. To bring DMD CRISPR... Cas9s such as eSpCas9 and SpCas9-HF should be explored to reduce off-target editing. Short-term CRISPR...

...rural Africa Once this position ended, I wanted to explore another area which I was really passionate about...returning from Ghana at the beginning of 2010, I explored options for going into parasitology but another... for young scientists and PhD students to also explore a range of alternative careers outside of acidemia...

...is a PhD trained scientist at the University of Colorado, Anschutz Medical Campus.  He studies viruses ...currently a postdoctoral fellow at the University of Colorado Anschutz Medical Campus in Aurora, CO where she.... Erin is also the President of Project Bridge Colorado, an advocacy initiative that seeks to bridge the...

...cytometry experiment uses 2 or 3 different fluorescent colors each measuring a different target. Flow cytometers...versatile and some can accommodate up to 30 different colors. This lends flow cytometry particularly well to...causing dead cells to fluoresce.  When running a multicolor flow cytometry experiment, users must also prepare...

...an action potential. Halorhodopsins (HR) are green/yellow-light sensitive chloride ion pumps, activation...activation of which results in efflux of chloride ions, subsequent hyperpolarization, and inhibition of action...

...Cas9 in the apo state (PDB ID 4CMP) with domains colored as in Figure 1. HNH, red; RuvC, orange; CTD, yellow...mismatches between base pairs 18 and 20.   Pro tip! The Taylor lab mutated the residues in this loop to produce... L., Jung, K., McCool, R. S., Johnson, K. A., & Taylor, D. W. (2022). Structural basis for mismatch surveillance...

...United States including green cards that are well-tailored to meet the needs of researchers. The categories...and the arts, and requiring the attainment of a bachelor's degree or its equivalent as a minimum (8 U.S....). The foreign worker must possess at least a bachelor's degree or its equivalent and state licensure,...

....”- Joanne Kamens, Executive Director Career exploration “You can't wait for opportunities to fall in .... But there were a few teachers and 'guidance counselors' along the way who thought it was risky, "not...

...methods in this blog post. 8. Chromoproteins: Colorful Proteins For Molecular Biology Experiments by ...the fluorescent protein family and they generate color! They've been adapted for many molecular biology...

... who passed away in 2008. His work exposes and explores the hidden structures within flowers with the ...technique provides scientist with a new method to explore the inner structures of delicate plants and other...

...]. With the advent of CRISPR/Cas9, we began to explore the paired double-strand break (DSB) approach at...ectopic reporter assays. We have been interested in exploring the function of non-coding regulatory elements...

...fluorophore tagging vectors for your single or multi-color imaging experiments. Interested in epitope tags?... Julian Taylor-Parker...

...responsibilities on the QC team, I was able to explore other parts of Addgene. This was done through the.... Specifically as a co-op, it is encouraged to explore different teams and work on projects there. At ...

...highlight our blog, daily updates, and offer better exploration throughout the site.  Blugene gets a make-over...AAV friend.  We encourage you to take a look and explore the new homepage. We hope every time you come to...

...and drag and drop an icon onto the workspace. The color and size of icons are adjustable. New icons can ...scientific graphics. The tools settings let you tailor your search to particular types of images. For ...

...opportunities for promotion. It’s not uncommon for bachelor’s and master’s level scientists to have job titles...Now? Academic v. Non-Academic Career Decisions Explore alternative science career options Resources on...

...science. “It ended up we moved to Colorado for my husband’s postdoc. Colorado is growing in the biotech sphere...