We narrowed to 722 results for: MAK

...over-diluting, and make sure to remeasure your titer after diluting to confirm. Luke Makana Hanley is a Research...Check out this post for a refresher on AAV titers Making contact with a cell Although an individual AAV ...see the dwindling proportion of AAV particles that make it through each part of the cell on their way to...additional best practices. Keep your cells happy - Make sure your cells are as healthy as can be to prepare...filter - When using a concentration filter column, make sure to rinse off the surface of the filter membrane...

...running one, you’ll know there’s a lot of choices to make when designing the experiment. What detection method...    Figure 1: The technical decisions to make when designing a western blot.  Psst! Looking...antibodies are relatively cheap and easy to source, making it easy to switch from one readout method to another...blocking buffer and a recommended concentration. That makes the choice easy! Protein-based blockers include ...Chemiluminescence kits When activating your enzyme, make sure to choose a chemilumescence kit appropriately...greater dynamic range than chemiluminescence enzymes, making it easier to accurately visualize proteins of wildly...proteins diffuse semiradially during the transfer, making membranes inherently “fuzzier” than gels. Quantification...

...variable actually makes a big difference for the robustness of the findings.   How do we make research more... noise in the process of sampling and inference-making. Even perfect transparent and rigorous studies ...with a specific statistical test.  Preregistration makes clear the distinction between hypothesis testing...With Registered Reports, the authors are able to make that clear distinction between hypothesis testing...Furthermore, the journal and reviewers are incentivized to make sure that any possible null is really a true null...

...we choose topics for the Addgene blog? Or why we make certain videos? In this post, we invite you behind...experiences from thousands of laboratories that makes up our Help Center, blog, website, social media,...process led to quick dives back into the literature to make sure absolutely everything was correct. Final touches...between our content team and our graphic designer to make sure things are clear, accurate, and in line with...shown.   However, for our blog posts, we usually make figures in BioRender or use appropriately licensed...review to catch those pesky typos, and hit publish. Making content is an iterative process. We are constantly...

... 2015. What makes lentiCRISPR v2 great? It produces a ~10 fold higher titer virus, making it ideal for...a point mutation in the puromycin cassette that makes it less efficient in some cell lines. pSpCas9(BB...V2.0 was made available through Addgene in 2015, making this the most recently available plasmid in the...a point mutation in the puromycin cassette that makes it less efficient in some cell lines so it may be... thank all of our depositors and requestors for making their reagents available and for supporting scientific...

...similar to previously discovered Cas13 enzymes - what makes these orthologs so special? The first property is...Cas13d enzymes have a median length of ~930aa - making them 17-26% smaller than other Cas13s and a whopping...whopping 33% smaller than Cas9! Their small size makes then easy to package in low-capacity vectors like...broader regulators of Cas13d activity. This property makes WYL proteins an intriguing counterpart to anti-CRISPR... yielded >80% knockdown. It seems that CasRx may make it possible to target essentially any RNA in a cell...and we at Addgene appreciate our depositors for making their tools available to the broader community....

...cargo is designed to make you sick…well, you feel it. But the same features that make these familiar viruses... and deposit a DNA/RNA cargo into their targets makes them attractive and tractable tools for genome engineering...essentially DNA (or RNA) delivery vectors, so what makes them better than the alternatives? Well, for one...Secondly, some viruses have a low immunogenicity which makes them ideal for applications with whole organisms... when introduced into an organism. This feature makes them an attractive tool for human subject delivery...

... aided design) drawings of their devices, which makes it very difficult to adapt the device to one's own...2020) or openUC2 (Diederich et. al., 2020) aim to make microscopy as a tool accessible to practically everyone...growing number of "community-driven" software that make everyday microscopy much easier. On platforms such...sources such as Amazon, Ebay, or Aliexpress, which makes it difficult to develop accessible open-source hardware...Distributing hardware via globally established makerspace networks and training users via workshops, seminars...newly developed techniques are the key enablers for making assemblies open-source. The sharing of digital ...

...light-gated ion channels such as channelrhodopsins (ChRs) makes them light-responsive. When light shines on excitatory...causes it to fire. Created with BioRender.com. To make channelrhodopsin-based optogenetics minimally-invasive...channelrhodopsins. Their new channelrhodopsins, called ChRgers, make the brain a little easier to access with optogenetics...injection. This lower multiplicity of infection (MOI) makes it challenging for ChR2(H134R) to function because...showed that ChRger2 stronger photocurrent properties make it better suited for systemic delivery with PHP....TL;DR The new channelrhodopsins, called ChRgers, make it easier and less invasive to use optogenetics ...

... unique cutting features and evolved PAM sites, making Cas9 an adaptable choice for many genome engineering... into crRNA). The size and simplicity of Cas12a makes it easier to package for therapeutic delivery by... to give it a go? The high specificity of Cas14 makes it a promising detection tool for rare, often hard...unlike Cas9, it targets RNA instead of DNA. This makes it particularly useful in generating transient edits...target sequence. The RNA-targeting ability of Cas13 makes it perfect for targeting mRNA and useful as a mechanism...targeting abilities of the other Cas family members make for useful implements in the scientist toolbox. ...

...your way. Label your tubes with a label maker. Label makers generate far more legible labels than your...into the lab wishing there was a machine that could make your life a hundred times easier. We’ve certainly...Addgene recently started automating a few protocols to make standard lab processes easier and more efficient...really liked that Beckman was flexible and willing to make removable parts to fit on the deck of the Biomek...pick up 8 tips and position the tips indepently making it easy to spread out your samples. This can be...

...mechanical work for editing. Class 1 CRISPR systems make up the majority of CRISPR-based immune systems in...broken down into different types, with Cas3 systems making up type I, the most abundant type in the Class ...away at both the non-target and target strands by making cuts every so often. Cutting both strands ensures... for Cas3’s biggest trick. Cas3 likes to bluff, making you into think it’s more powerful than it is. The...issues with expressing all Cascade components can make Cas9 easier to work with than Cas3. Future targets...1477. https://doi.org/10.1038/s41587-019-0310-0 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,...

... we’d like to make sure they are present at a very low concentration. So how do we make sure to catch ...an experiment because of poor reagent quality. To make sure our AAV vectors are of the highest quality,... mission of accelerating scientific research. To make sure that adeno-associated viral (AAV) vectors available...robots. Unfortunately, humans have been known to make mistakes. What if a tube is mislabeled or someone...K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020...

...be “let’s try to make this STEM activity fun,” but rather “let’s see how we can make this fun activity...time is giving fewer examples and think of a way to make this section more interactive. If you can think ...good analogy that involves student participation, make it happen! How should we use CRISPR? How shouldn...students that scientific history was very much in the making. One of the exciting, and challenging, aspects ... Perhaps you have young children and you want to make sure their STEM education is supplemented. A 2009...

...Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J,...Non-SpCas9's bind a variety of PAM sequences, which makes them useful when no suitable SpCas9 PAM sequence...non-SpCas9's may have other characteristics that make them more useful than SpCas9. For example, Cas9 .... BV3L6 Cas12a, AsCpf1, uses a 5'-TTN PAM which makes it easier to target AT-rich genomes (Kleinstiver...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...

...to be a part of projects that connect people and make you work in a collaborative environment. Think of...conversations about your ideas and don’t be afraid to make something concrete happen. You may have more willing... institutions personally on conference calls and make sure to maintain these relationships by keeping ...organization. You can meet collaborators  for lunch, make a point of talking to them at a seminars, or even...from an internal to an external connection is to make sure you keep open lines of communication with colleagues...see interesting events, articles and newsletters I make sure to share them with a short note - this creates...

...reliable and robust high quality viral vector service, making an array of ready-to-use viral vectors available... prioritization process to decide which tools we make available. This prioritization happens for good...of our most popular ready-to-use viral vectors, making up almost 10% of our requests by 2019 (Figure 5A...which preps to include in our service allows us to make ready-to-use viral preps that are available quickly...Hub before selecting an AAV can help a researcher make a more informed choice on which AAV to request and...since, we’ve learned so much about what it takes to make these viral vectors not only available, but accessible...

... Denotes the presence of an additional plasmid-- make sure to maintain this by growing on appropriate ...Induction timing is important, as you typically want to make sure your cells have first reached an appropriate...exponential growth phase are alive and healthy, which makes them ideal for protein expression. If you wait too... as there are not enough cells in the culture to make protein.  The DE3 lysogen/T7 promoter combination...positive selection and a p15A origin of replication, making it incompatible with other p15A plasmids. pLys ...Double-check your plasmid backbone and expression strain to make sure they are compatible. An arabinose-inducible...

... can promise is that my suggestions can help you make the best case for your candidacy. A major reason...complains that a seminar was too easy to understand. Make your seminar accessible. Let the experts ask you...minutes of the chalk talk. Realize that it is rare to make it through the details of all of the aims of a chalk...points you want to convey for each section or aim. Make sure those get priority. The fine details for aficionados...examples are not especially helpful. I set out to make something practical and accessible. If there's something...postdoc salaries all too well. If there's a group to make money from, postdocs are not that group. If you ...

...even one less run a week makes a big difference. Clean out freezers so you can make room and don’t have to..., check out my Recycling and Innovations posts. Making small changes in your daily habits can feel ineffective...sustainability problem, how can one little change make a difference? Individual changes to our behavior...wouldn’t leave your oven on all day to save time making dinner at night, so why leave your lab equipment...