We narrowed to 97 results for: Mycs

1. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   Published

   March 28, 2018, 6:52 p.m.

   ...bacteria where cleavage leads to the loss of spectinomycin resistance. xCas9 3.7 showed the broadest PAM...

2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

   Type

   Blog Post

   Published

   April 4, 2019, 12:53 p.m.

   ...the human gut bacteria B. thetaiotaomicron, Mycobacterium, Pseudomonas, and E. coli. However, many non-model...

3. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...Global analysis of protein homomerization in Saccharomyces cerevisiae. Genome Res 29:135–145. https://doi.org...

4. Droplet Digital PCR for AAV Quantitation

   Type

   Blog Post

   Published

   Jan. 16, 2020, 2:18 p.m.

   ...Song, Neng, et al. "Detection of circulating Mycobacterium tuberculosis-specific DNA by droplet digital...

5. Tips for Screening with Yeast Two Hybrid Systems

   Type

   Blog Post

   Published

   Oct. 22, 2015, 2:30 p.m.

   ...Two hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively ...

6. Tips from the Repository Trenches: Using Barcodes to Track Samples

   Type

   Blog Post

   Published

   Oct. 6, 2015, 2:30 p.m.

   ...majority of plasmids contain either an ampicillin or kanamycin resistance gene. Every step along the way involves...

7. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

   Type

   Blog Post

   Published

   July 7, 2020, 1:15 p.m.

   ... a mScarlet reporter, luciferase reporter, and neomycin resistance reporter. Image adapted from Katti ...

8. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

   Type

   Blog Post

   Published

   July 23, 2019, 12:46 p.m.

   ...check out these two references: Dayton, Robert D., Mychal S. Grames, and Ronald L. Klein. "More expansive...

9. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

   Type

   Blog Post

   Published

   May 2, 2019, 12:41 p.m.

   ...So, think beyond Drosophila melanogaster or Saccharomyces cerevisiae (both fine research organisms) -  ...

10. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ... vectors for CROPseq-multi using selection by Puromycin, Zeocin®, or nuclear expression of BFP2.   ...

11. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    Published

    Oct. 19, 2017, 1:54 p.m.

    ...Sadowski. 1985. “The FLP Recombinase of the Saccharomyces Cerevisiae 2 Micron Plasmid Attaches Covalently...

12. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ...Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae. Paques F and Haber JE. Microbiology...

13. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ...et al. "Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to resistance....

14. Plasmids 101: Repressible Promoters

    Type

    Blog Post

    Published

    Dec. 20, 2022, 2:15 p.m.

    ...genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS Yeast Res. 12(2) (2012): 197...

15. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type

    Blog Post

    Published

    April 25, 2017, 2:30 p.m.

    ...protein‐tracking studies in the budding yeast Saccharomyces cerevisiae." Yeast 25.9 (2008): 651-659. PubMed...

16. Plasmids 101: Modular Cloning Applications and Kits

    Type

    Blog Post

    Published

    May 14, 2024, 1:15 p.m.

    ...Toolkit for Extensive and Flexible Engineering of Saccharomyces Cerevisiae.” ACS Synthetic Biology, vol. 12,...

17. CRISPR 101: Cytosine Transversion Editors

    Type

    Blog Post

    Published

    March 25, 2025, 1:15 p.m.

    ...In addition to using a new UNG ortholog from Mycobacterium smegmatis, termed UdgX, they found three proteins...