We narrowed to 130 results for: NAP
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TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....grows on a daily basis, the lists below provide a snapshot of relevant resources and a good jumping off point...
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A Deep Dive into BioBuilder
TypeBlog PostPublishedOct. 11, 2017, 1:44 p.m....and in more than 30 countries around the world. Snapshots of the BioBuilder experience With its intentionally... -
Fluorescent Proteins 101: Luciferases
TypeBlog PostPublishedJuly 22, 2025, 1:15 p.m.... (luciferin),730–743 (analogs) 64 Luciferin or naphthyl-luciferin analogs ATP No Deep sea copepod (Gaussia... -
Plasmids 101: Screens vs. Selections
TypeBlog PostPublishedNov. 10, 2022, 2:15 p.m....lot in common. In fact, selections are often inappropriately categorized as screens! Why the confusion? ... -
Antibodies 101: ChIP
TypeBlog PostPublishedMarch 10, 2022, 3:25 p.m....ChIP experiment! Image courtesy of https://www.wannapik.com/ Even though ChIP requires a larger ... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m....anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag fusions, while the anti-HA frankenbodies... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m.... neurons and six hundred trillion connections (synapses.) In this post we’ll describe the AAV retrograde... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m....optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling, et. al, "Robotic microscopy for... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....base editor corrects early stop codons within T7 RNAP. A phage encoding the deaminase TadA-7.10 A mutagenesis... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog PostPublishedJune 30, 2016, 2:30 p.m....illustrates how sequence visualization software (we used Snapgene here) can help you assess whether your restriction... -
RANbodies: Reporter Nanobody Fusions
TypeBlog PostPublishedApril 10, 2018, 12:56 p.m.... GFP, RFP) Detecting GFP reconstitution across synaptic partners (GRASP) assays No antibody required... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog PostPublishedOct. 1, 2019, 1:21 p.m....updates, or click bait of any kind. For more on inappropriate content see this article. However, do comment... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....These products, namely RNAlater® from Thermo and RNAProtect® from Qiagen, are ammonium sulfate-based solutions... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m....examples include Benchling, Serial Cloner, and Snapgene), and save these to a shared computer or server... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....spider silk, or the activity of a protein in a synapse. What kinds of tools enable us to measure these... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog PostPublishedAug. 6, 2019, 12:55 p.m....and Nancy Kleckner. "Recombination, pairing, and synapsis of homologs during meiosis." Cold Spring Harbor... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 3, 2022, 1:15 p.m....for the visualization of endogenous proteins and synapses. Feng S et al. Commun Biol. 2019 Bo Huang Near-Infrared... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....selectable markers by detecting common features using Snapgene. If we find information that differs from what... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m....neurons, and are especially abundant in neuronal synapses. shRNA silencing of one circular RNA they have... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m....EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP) tags, a selectable marker (floxed Cbr-unc-119) ...