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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 469 results for: NOL

Showing: 81 - 100 of 469 results

  1. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    Published
    May 4, 2021, 1:15 p.m.
    ...revolution for antibodies. Recombinant antibody technology makes it easy for scientists to share, remix,...proposed a shift to recombinant DNA-based antibody technologies (Bradbury & Plückthun, 2015a).   Figure...monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife 8: . https://doi.org/10.7554...srep31730 Tsuruta LR, dos ML, Moro AM (2018) Display Technologies for the Selection of Monoclonal Antibodies for...

  2. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...ideas (how to stop rhino horn poaching using biotechnology) were thrown on the table and dissected with...reputation. The public is can be fearful of this technology and angry at the agriculture companies that produce...opportunity has given us the chance to develop the technology we will need, and the business training to make...and Biochemistry from the Illinois Institute of Technology in 2004, she accepted a commission in the United...

  3. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...qPCR Using SYBR Green TechnologyAAV Titration by ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV...Figure 1: Overview of key steps of AAV production, iodixanol gradient purification, and AAV titration by qPCR...purified by density gradient centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge. During the...

  4. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...Prime editing is a versatile genome editing technology that allows precise modifications of DNA (replacements...features and DNA repair determinants. Nature Biotechnology 2023, 1–11. https://doi.org/10.1038/s41587-023...and product purity by deep learning. Nature Biotechnology, 41, 1151–1159. https://doi.org/10.1038/s41587...efficiency across diverse chromatin contexts. Nature Biotechnology. https://doi.org/10.1038/s41587-024-02268-2 ...

  5. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ... last 5 years to incorporate more science and technology into the school curriculum. Our Parent Teacher...and the internet before it, genome engineering technology is moving along much faster than it can be officially...sugarcoat the potential dangers of people using the technology in an unethical manner, but I also tried not ... timely.” Genome engineering, and many other technologies and fields in biology, are moving so quickly...

  6. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...must stay current with literature and emerging technologies to remain competitive and relevant. Enter: the...department, we had two recurring journal clubs on Immunology and Microbial Pathogenesis that occurred once...spirited and focused as opposed to a wandering monologue?  Assuming the feedback is generally positive ... Will Arnold...

  7. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...Figure 1). Citorik et al. targeted beta-lactam and quinolone resistance genes in E. coli using plasmid and ...plasmid. In the case of the genomically encoded quinolone resistance, mediated by a single base pair change...efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed: PMID 252409288...produce sequence-specific antimicrobials.” Nature Biotechnol. 32(11): 1146-50. (2014). PubMed: PMID 25282355...

  8. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ... for clinical diagnostics, genotyping, and biotechnological advancements. Many methods of nucleic acid...Biosciences has been started based on DETECTR technology with the mission to “provide a CRISPR-based platform...strip format. They published this in Nature Biotechnology. Like the SHERLOCK detection system, DETECTR...CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Chen...

  9. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...the AOX1 promoter whose expression is induced by methanol. Once linearized, the delivery vector can be integrated...Zeocin resistance. Then, counter-selection with methanol medium selects for those cells that have removed... In cells where recombination has not occured, methanol induces the production of the toxic MazF leading...Vibrio cholerae vaccine strain CVD 103-HgR." FEMS Immunology & Medical Microbiology 57.1 (2009): 69-79. PubMed...

  10. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...reports suggest rapidly freezing virus in a dry ice/ethanol bath or liquid nitrogen prior to storing. Know ...effects, detection, elimination, prevention.” Cytotechnology 39.2 (2002). 75-90. Pubmed PMID: 19003295. ...oncoretroviral and lentiviral gene therapy vectors.” Biotechnology Advances 24.3 (2006). 321-37. Pubmed PMID: 16448798... effect of sera and polycations.” Molecular Biotechnology 53.3 (2013). 308-14. Pubmed PMID: 22407723. ...

  11. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...it comes to altering gene expression, CRISPR technologies have gradually taken over as the preferred method... their promoters in human and mouse. Nature Biotechnology, 35(9), 872–878. https://doi.org/10.1038/nbt... constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org/10.1186/1472-6750-6-1...mechanisms of actions, and circulation. Frontiers in Endocrinology, 9. https://doi.org/10.3389/fendo.2018.00402...

  12. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ..., 2013). APEX catalyzes the oxidation of biotin-phenol to the short-lived biotin-phenoxyl radical in the... 2012). It is important to note that the biotin-phenol reagent can be toxic to living cells thus APEX ...living cells and organisms with TurboID." Nature biotechnology 36.9 (2018): 880. PubMed PMID: 30125270. PubMed...encoded reporter for electron microscopy." Nature biotechnology 30.11 (2012): 1143. PubMed PMID: 23086203. PubMed...

  13. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...alternatives to restriction enzyme cloning. These newer technologies have become more and more common, and for good...Gibson assembly combined with the popular CRISPR technology and is described in a recent publication by the...technically challenging. Other homology based technologies We've described Sequence and Ligation Independent... Kahl, L. J. & Endy, D. A survey of enabling technologies in synthetic biology. J. Biol. Eng. 2013; 7,...

  14. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...founded.  This commercialization of gene editing technology led to the development of many important medical...double-strand breaks (DSBs) in the genome. These technologies rely on the cellular DNA repair machinery, which...genetic engineering and open advertisement of the technology on various non-scientific platforms, CRISPR has...perspective of the current. Although genome editing technologies have brought a renaissance in the field of health...

  15. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...sports photographer at the Super Bowl, a medical technologist taking an x-ray, or a biologist imaging the ...microscopes (see Table 1). Thin dynamic samples Ex: monolayers of live cells with fluorescently labelled, motile...doses to the cells. Thin static samples Ex: Fixed monolayers of cells or thin (<15 μm) tissue sections and...imaging technique but recent advances in scanner technology and parallelization (7) have allowed for real-time...

  16. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...classification system for the array of CRISPR technologies. Learning the general principles of this classification...the first taught when introducing CRISPR as a technology. There are three subtypes, A–C, with the beloved...Y. (2018). Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing. Cell ...Yoshimi, K., & Mashimo, T. (2022b). Genome editing technology and applications with the type I CRISPR system...

  17. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ...their hot-off-the-presses papers, the awesome new technology they’re utilizing, and the tricks they’ve used...plasmids in your own lab. Summaries of the latest technologies and breakthrough research. Insights about ...a special focus on cloning and plasmid-based technologies. Commentary about scientific sharing and the...

  18. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ...the Addgene YouTube page! AAV purification by iodixanol gradient ultracentrifugation This video goes over... over AAV vector purification using an iodixanol column gradient. We'll show the loading and unloading...instructions. AAV titration by qPCR using SYBR green technology Here’s some advice on how to successfully set...

  19. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ... and Diplomacy, with a focus on international technology management, from the Fletcher School at Tufts...investment banking analyst at Credit Suisse’s Technology Group in Palo Alto, CA.  Benjie Chen is a co-founder...and networks. Benjie was Addgene's Director of Technology for 7 years, and is now Chief Software Engineer...

  20. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...; Hellemans J; Huggett J; Kubista M; Mueller R; Nolan T; Pfaffl MW; Shipley GL; Vandesompele J; Wittwer...abundances using SYBR Green I and real-time RT-PCR. J. Immunol. Methods 283:291–306.Panina, Y., Germond, A., Masui...Reproducible Data the First Time, Trends in Biotechnology, Volume 37, Issue 7, 2019, Pages 761-774, SSN... for low copy number target DNA. Journal of Immunological Methods 270 (2002) 119 – 133Tichopad A; Dilger...
Showing: 81 - 100 of 469 results