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We narrowed to 301 results for: Nov

Showing: 81 - 100 of 301 results

  1. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...Berkeley, provides a new approach for generating novel genetic variants in bacteria. Read on to learn about...of EvolvR The Dueber Lab used EvolvR to discover novel genotypes that result in a spectinomycin resistant...spectinomycin, rpsE. These results not only uncovered novel resistance genotypes, but also provide a better ...

  2. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...; synthetic biology is required to achieve this novel and unique phenotype. It’s an unexpected use of ...creating new forums and opportunities for open innovation in plant synthetic biology. In our case, we selected...off patent, or not patented, and building our own novel pieces where applicable. We will also be working...

  3. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...one, is a sterling example of collaboration and innovation between legal and scientific fields.” Thanks ... every day for their dedication, flexibility, innovative thinking, and openness to team work.   Company...positions. We have an awesome talent pipeline. Turnover at Addgene is about half of what is standard in...

  4. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...Research Strategy highlights i) the significance and innovation of the project and ii) how you, the expert, will...writing a proposal to test hypotheses and develop novel technologies! If data from a publication or preliminary...funding. Convince the readers why and how your innovation will address a variety of challenges and impact...

  5. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ... in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). The least ...is usually classified as a BSL-1 safety level. Adenovirus is similar to AAV in that it does not integrate...integrate into the host’s genome. However, some adenovirus strains are known to cause respiratory diseases ...

  6. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...assembled into a circularized plasmid sequence de novo (without the use of a reference sequence) using ...the plasmid, QC scientists will first align the de novo NGS result to the deposited sequence(s) in SnapGene...if one was provided. If no reference is given, de novo assembly in Geneious can still provide additional...

  7. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...replicate itself and depends on helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient...a “helper-free” system, in which the required adenoviral helper genes are supplied on two different packaging..., J. (1999). Titration of AAV-2 particles via a novel capsid ELISA: Packaging of genomes can limit production...

  8. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...expressing your gene, especially if you are studying a novel gene, is not always a straightforward process. Rather... of interest, scientists have instead developed novel methods to co-express your gene along with a reporter...more time developing the clever experiments and innovative solutions necessary for advancing the field. ...

  9. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...locomotor sensitization and might open new ways for a novel therapeutic approach against drug addiction. Anxiety...illuminated ones (risky). They also prefer to explore novel environments by initially moving along a wall (protected...bright place, driven by their motivation to explore novel environments, rather than being inhibited by fear...

  10. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...members are linked to viral susceptibility (mouse adenovirus in mouse; HIV1, West Nile, and Influenza in humans...finding illustrates how the method used to select novel AAV capsid variants could unintentionally limit ...Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP. B capsids." bioRxiv...

  11. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...co-founded by Kaspar, acquired by Novartis and rebranded to Novartis Gene Therapies, and Taysha Gene Therapies... in tackling the root cause of Rett Syndrome in novel ways.  Addgene’s MECP2 Plasmid Collection and Resource...

  12. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ... publish on the health and economic benefits of novel drugs. Commercial Affairs teams make up a large ...for cancer with chemotherapies, or developing innovative mobile phone applications and services that help...the more challenging parts of the job? With fast turnover of projects, pressure to maintain consistently...

  13. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...developed NIR split probes (Tchekanda et al., 2014, Filonov et al., 2013). FRET biosensors were developed based... interactions. Nat Methods 11, 641-644 (2014). Filonov, G.S. & Verkhusha, V.V. A near-infrared BiFC reporter... vivo imaging. Nat Methods 10, 751-754 (2013). Filonov, G.S. et al. Bright and stable near-infrared fluorescent...

  14. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...inhibited. Recently, we at the Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research...recombinant antibodies (rAbs) either produced de novo using yeast display technology or produced from ...a science writer at the Institute for Protein Innovation. She writes about all things IPI, from the scientists...

  15. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...for inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives." Proceedings...Academy of Sciences 113.13 (2016): 3509-3514. Tikhonova, Elena B., et al. "A transcription blocker isolated...metalloproteinase: López-Pelegrín, Mar, et al. "A novel mechanism of latency in matrix metalloproteinases...

  16. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...vesicular stomatitis virus (VSV; Beier et al., 2011), adenovirus, and adeno-associated virus (AAV), have been ...the California Institute of Technology generated novel AAVs (Chan et al., 2017) that express one of a few...type-specific genetic and optogenetic tools reveal novel hippocampal CA2 circuits. Nat Neurosci 17(2), 269...

  17. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...Mark your calendars for November 16-20, 2020 for #DepositorWeek on Twitter!  That’s today through Friday...presented in this blog post includes data until November 9, 2020. Between then and the posting of the blog...

  18. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    Published
    Dec. 20, 2016, 3:30 p.m.
    ...University of Edinburgh where he worked on developing novel systems for bioconversion of cellulosic biomass ...spend some time learning and doing research at Novartis. Listen to learn how Kwabena plans to use his ...

  19. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ...Colorado, Berlin, Atlanta, Ottawa, Norwich, and Hannover, we raised money for the Superhero 5k and, of ...Increased Postdoc Wages I hope you all have a great November! Tyler J. Ford ...

  20. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...vector. Moreover, the screen identified putative novel regulators of CD8+ T cell responses in tumors, demonstrating... demonstrating that this system can also reveal novel pathways. The FITS deposit also includes pLX_305...
Showing: 81 - 100 of 301 results