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...Escherichia coli bacteria. It’s hard to count the number of different commercial strains of E. coli currently... If you were to include customized strains, the number is probably in the thousands! The goal of this ... (F-) contain the fertility plasmid. A low copy-number plasmid, encodes proteins needed for bacterial ...

...’s wise to quickly assess whether a significant number of cells have been edited. For many experiments...population. You can additionally calculate the minimum number of clones you would have to screen to identify ...occur, allowing you to Sanger sequence a manageable number of the higher probability locations. If you’re ...

...achieved by traditional pharmacologic methods. A number of strategies have been developed for utilizing...cellular activity and physiology. However, there are a number of factors that can wreak havoc on your experiments...neuronal signaling or cancer pathways there are a number of optogenetic tools now available to help light...

...repeats (Ribeiro et al., 2009), and at high copy numbers (Williams, Carnes, and Hodgson, 2009). More research...larger plasmids are also maintained at lower copy numbers within bacterial cells, and bacterial cells with...Summers DK, Sherratt DJ. Multimerization of high copy number plasmids causes instability: CoIE1 encodes a determinant...

...a potential limitation in a study. Strength in numbers If you assess your off-target frequency to sit ...whatever your editing event outcome is at a reasonable number, the likelihood of an off-target confounding your... the same principle holds true - the higher the number of distinct editing events, the lower the likelihood...

...gold-standard” for LOF studies, the high gene copy number found in plants makes traditional knockouts unattractive...reversibly exert their effect independent of gene copy number. RNA interference (RNAi) is a conserved eukaryotic...thereby limiting CRISPR’s effectiveness at high copy numbers and genomic loci with low accessibility. Furthermore...

...-containing cells versus total cell number to determine the number of B cells in that sample. Alternatively...called an event, and assigns each event to a channel number based on intensity. Higher intensity events are...

...knockout library is available alongside a growing number of CRISPR libraries from Addgene’s repository that...by utilizing pre-assembled trimers to reduce the number of cloning steps. The FusX system was verified ...like CLIP require a specific antibody and a large number of cells to analyze. A new technique from Michael...different promoters in the kit and by altering the number of tandemly expressed gRNAs respectively) and 2...

...an average indel frequency of only 1.1%. These numbers are a vast improvement over Cas9-mediated HDR for...editors with distinct PAM sequences, expanding the number of available target sites for base editing (Kim...BE3 editing window variants   Greatly expands the number of target loci for base editing Rees et al., ...

... dates back to 1975 and is based on the average number of citations for recent articles. Of course, a ... research" (2013) Scientific Reports 3, Article number: 1649 doi:10.1038/srep01649    ...

... system that generates vectors containing high numbers of gRNAs in one overnight reaction. STAgR is highly...customizable as vector backbones, and gRNA structure, numbers, and promoters can all be chosen. Read the paper...

...organizational skills provide great training for a number of alternative careers in science. In return, the...to expand the conference to accommodate a larger number of attendees. This first-year event’s success is...

...action that can result in the initiation of a vast number of signaling cascades, inducing different cellular...for? The Roth lab used the TRUPATH plasmids in a number of different experimental approaches. They first...

...At first, the numbers I presented at biweekly meetings didn’t seem much more than numbers. As time passed...

[email protected] Entries include accession number, species, and assigned category (cancerous, hybridoma...interest. You can filter CLASTR results by minimal number of markers and similarity score match. This tool...

...expression of these three genes with different copy numbers, producing dozens of color hues (Livet et al., ...fluorescent proteins is only possible at low copy number ranges, resulting in low fluorescent protein level...

...to assess purity. For labs that produce a small number of AAV preparations this level of quality control... viral vector core facilities producing a large number of viral preparations in parallel, there is a very...

... Numbers in the large colored circles are rough approximations of the total number of CRISPR plasmids...

... authors for additional information (accession numbers, reagent details, author contributions, etc.) before...priority list. There has been progress—for example, a number of funders (e.g., the NIH) now look for data management...

...processes. Chemical effectors are suboptimal for a number of reasons, including their potential toxicity ...factors. These bulky systems were spread over a number of plasmids, with one RR (ompR) encoded chromosomally...