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Showing: 81 - 100 of 141 results

  4. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...finishes repair of most types of breaks in tens of minutes – an order of magnitude faster than HDR. NHEJ is...

  6. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...you scale up, the harder it is to throw in last minute experiments or changes. Fun fact: Addgene’s high-throughput...

  13. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...as digestion and ligation can be done in one 30-minute reaction. The destination vector and entry vector...

  14. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...organs, which then return information such as nutritional status. Within the animal kingdom, this information...

  16. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...These editing frequencies can be achieved with a 30-minute incubation, even with multiplexed guides. ...

  17. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...activation is delayed and takes on the order of minutes. Studies looking at immediate effects of specific...

  19. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...activated, Cas9 cleavage can induce DSBs within minutes. New CRISPR plasmids for Borrelia burgdorferi from...
Showing: 81 - 100 of 141 results