We narrowed to 123 results for: PTS;

... LTR) and Rev (facilitates nuclear export of transcripts). A different viral envelope protein (Env), usually...

...analogy that would help them grasp some of the key concepts (Fig. 2). One thing I will likely change for next...

...the RESCUE system’s ability to edit endogenous transcripts finding up to 42% editing efficiency. They note...

...and communicating science for over two decades, attempts to answer it in five paragraphs, with examples...

...’ results is available not through published manuscripts, but through word of mouth or old lab notebooks...

...isothiocyanate for the gel extraction protocol) disrupts the DNA’s hydrogen bonds with water molecules ...

... prone to create secondary structures in RNA transcripts Effects on transcription (remember, it’s not ...

...access on my phone. I use it for everything from receipts to blogs to recipes. I am not paid to say this...

...sequence as specified by the crRNA sequence, it adopts an enzymatically “active” state rather than reverting...

...Resources on the Addgene Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing ...

... vector.   Figure 2: The Gateway system adopts phage integration into the BP and LR reactions....

...opposing behaviors are possible: active swimming in attempts to escape, or passive floating on the water surface...

...use of introns, which will be removed from the transcripts in the plant but not from Agrobacterium. Test...

...rise to a mutant TadA (TadA*) that satisfactorily accepts DNA as its substrate (see figure 2).          The...

...into one AAV, Cas9 must be made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9 (~...

...the lab introduced a 43 base-pair insertion that disrupts fluorescence due to a frameshift. Restoration ...

...bacterial cloning glory, scientists have applied these concepts to deal with the more sinister plasmids of the...

...plasmid sequence, it moves to the next. If all three attempts fail, the QC team manually checks and assembles...

...Careful use of your glycerol stocks When your lab adopts the MoClo technology, you will quickly go through...

...the library sample, and any other information or scripts provided by the depositing lab for data analysis...