We narrowed to 118 results for: Pts
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TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....and communicating science for over two decades, attempts to answer it in five paragraphs, with examples...
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DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....isothiocyanate for the gel extraction protocol) disrupts the DNA’s hydrogen bonds with water molecules ... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....’ results is available not through published manuscripts, but through word of mouth or old lab notebooks... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m....analogy that would help them grasp some of the key concepts (Fig. 2). One thing I will likely change for next... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m.... LTR) and Rev (facilitates nuclear export of transcripts). A different viral envelope protein (Env), usually... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....into one AAV, Cas9 must be made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9 (~... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog PostPublishedJuly 7, 2020, 1:15 p.m....the lab introduced a 43 base-pair insertion that disrupts fluorescence due to a frameshift. Restoration ... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....bacterial cloning glory, scientists have applied these concepts to deal with the more sinister plasmids of the... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....opposing behaviors are possible: active swimming in attempts to escape, or passive floating on the water surface... -
Save Time with Transient Plant Leaf Transformations
TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m....use of introns, which will be removed from the transcripts in the plant but not from Agrobacterium. Test... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m....rise to a mutant TadA (TadA*) that satisfactorily accepts DNA as its substrate (see figure 2). The... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....development. We’ve shared what we’ve learned, through manuscripts (Ersing et al., 2023; Haery et al., 2019; McDade... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....the library sample, and any other information or scripts provided by the depositing lab for data analysis... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog PostPublishedSept. 21, 2017, 2:07 p.m....sequence as specified by the crRNA sequence, it adopts an enzymatically “active” state rather than reverting... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....Resources on the Addgene Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing ... -
Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m.... vector. Figure 2: The Gateway system adopts phage integration into the BP and LR reactions.... -
5 Reasons to Use Reddit for Science Communication
TypeBlog PostPublishedOct. 20, 2016, 2:30 p.m....access on my phone. I use it for everything from receipts to blogs to recipes. I am not paid to say this... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m....of several layers of support: A front-line team accepts any incoming requests and answers questions that... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....I would have done. And finally the stakeholder accepts the new feature as done and we’re onto the next... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m.... prone to create secondary structures in RNA transcripts Effects on transcription (remember, it’s not ...