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We narrowed to 837 results for: RING;

Showing: 81 - 100 of 837 results

  1. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...camera lens without having to physically rotate the rings around the lens. It allowed me to focus on the subject...constraints given. And there were many constraints during the COVID-19 pandemic. The Pandemic Pivot Back...Even Blugene is practicing social distancing and wearing their mask. But while the camera is here, the operator...chair not watching their actors live on set, but peering at a 7-inch wireless monitor. Wireless video technology...control, and extra hand sanitizer.    Cycle 4: Venturing into the Lab Design: The lab is the beating heart...heart of Addgene. It’s why we exist, storing plasmids, and distributing them to labs that need them. The...projects lined up, some that started production during the middle of lockdown, and some that will spin...

  2. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...spent hours in the biosafety cabinet with cloning rings or following labor-intensive limiting dilution protocols...is used for a variety of applications such as measuring target protein expression levels, assessing post-translational...uses 2 or 3 different fluorescent colors each measuring a different target. Flow cytometers however are...methanol, fix and permeabilize in a single step by denaturing proteins and dissolving lipids including those...specialized components that can divert a subset of cells. During FACS, the sample stream oscillates to generate ...be targeted with specific fluorescent antibodies during the flow cytometry experiment and will appear as...of yellow and orange from FITC and that from PE. During compensation the signal arising from a specific...

  3. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...like to go to. During the conference Take notes during talks. If you have questions during the talk, jot...conference depends on what you put into it before, during, and after the meeting. Let’s break it down into...Try to avoid jargon in this initial introduction. During the conference When you meet someone new at a conference... new method that you learned about at the talk, bring it to your next lab meeting. Don’t be afraid to ...for both instances. If your poster material is nearing publication, consider uploading your work to a ...poster) so your visitors can have a DOI to go to. During the conference Arrive at the poster session early...the board and meet your poster session neighbors. During the session, if someone comes up to my poster, ...

  4. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ... from Meiring et al., 2022, under CC-BY 4.0 license. A naturally occurring microtubule-severing protein...cells with physical structure, separate chromosomes during mitosis, support transport of organelles and vesicles...localized microtubule disassembly, Opto-katanin (Meiring et al., 2022), that overcomes many of those limitations...Opto-katanin induces fast, localized microtubule severing. A) Schematic of Opto-katanin constructs and activity...highly localized, rapid and reversible microtubule severing and has very low dark-state activity and toxicity...can be critical when, say, the gene is essential during development and a knockout is not feasible or when... One great way to do this is to use CRY2-CIB1 clustering. Fuse either CRY2 or CIB1 to your protein of ...

  5. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...This year, the IMP mentoring program extends its scope to the fields of Engineering, Economics, and Philology...internationalization and there is a lack of expert mentorship during undergraduate and graduate studies. Students also...Mentor Circle Programs at BWH and providing career mentoring to several students from Spain and all over the...in several countries, however programs like IMP, bring closer than ever (although not physically) the ideas...exist outside their immediate frontiers. Moreover, sharing a culture/country of origin with an international...2014-15, University of Valencia): “It is very inspiring to be in contact with mentors who are at a professional...the ability to accept feedback and adapt their mentoring styles to the mentee’s personality and professional...

  6. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ..., try lowering the voltage and using a longer run time.   Samples can run into empty neighboring lanes... two most common (non-technical) issues with transferring are smaller proteins going completely through...through the membrane or larger proteins not transferring completely onto the membrane. If you’re not sure ...sure if your protein of interest is over-transferring (going completely through your membrane to the other...other side), you can check by using two membranes during your transfer instead of one. If you see signal...both membranes, you know your protein is over-transferring! If you see very little signal, but you know... sample of a large protein, you may be under-transferring.  Membrane pore size Membranes come in different...

  7. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...Kleckner. "Recombination, pairing, and synapsis of homologs during meiosis." Cold Spring Harbor perspectives...generate double-mutant offspring. Crossing mice allows for the production of offspring that have a combination...generate a desired phenotype Instead, genetic engineering should be used to separately introduce each mutation...modification will be passed along to all of the offspring in the F1 generation, your breeding scheme should... two mutations that you want combined in your offspring. Genotyping involves getting a small piece of ...is homozygous for a different mutation, the F1 offspring will be either hemizygous or null at one locus...mice that were bred in your colony, as opposed to ordering control mice from a stock center. This serves ...

  8. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...tools available for monitoring how both the brain and visceral organs interact during various behaviors.... This is all an impressive engineering feat, but you’re probably wondering “did it actually work”? The...Neuromodulation During Free Behavior. 2020 42nd Annual International Conference of the IEEE Engineering in Medicine...continuously monitor it. How to address this? Through the pairing of materials scientists (Anikeeva lab) with gut-brain...physically hooked up to invasive, cumbersome systems during experiments – neither ideal for the intrepid researcher... fiber (Park et al., 2019). This provided an engineering road map of sorts, giving an idea of the stiffness...affect their function (the gut twists and contorts during the digestion of food, while brain tissue is relatively...

  9. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ... professor or hiring manager. Otherwise, use a gender-neutral greeting like “Dear Hiring Manager.” Highlight...If you are job searching, you may be wondering if you need to go to the effort of writing a cover letter...requires ddPCR skills. Your resume lists ddPCR, so the hiring manager knows that you have ddPCR experience. In...assay to answer an experimental question. Now, the hiring manager knows that you can design experiments with...briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based: if ... of the department] at [name of the university].During my thesis work on [phenomenon X], I read several...joining your team as a postdoctoral researcher. Dear Hiring Manager, I am writing to apply for [title of the...

  10. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...of “bringing tomorrow’s science into today’s classrooms,” BioBuilder answers the call by offering a variety...Tamer from BioBuilder, an innovative nonprofit bringing the study of synthetic biology into the hands ... Kuldell from MIT’s Department of Biological Engineering. Built primarily from her 20 years of college... boost interest and understanding around the engineering of biology. Participating schools are provided...BioBuilder inspires students and teachers to apply engineering principles to biological systems. Today, what...BioBuilder module allows students to explore how engineering fields rely on rapid prototyping to develop and...high school teams around the world who combine engineering approaches and scientific know-how to design/...

  11. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...and scientists - Culturing a scientific network We refer to the process of mentoring within a bin as Horizontal...and more. We are partnering with ASM and are working together with the society to bring an even more valuable...next component of Binning Singletons is when we bring all the bins together at a mixer. We call this Quorum...also sought expertise from many with experience mentoring in STEM, including Addgene’s Joanne Kamens. Expanding...Chicago.  Finding our place in networking and mentoring circles We looked at a lot of approaches scientific...scientific meetings have taken to provide mentoring. For example, Goldschmidt conference, Coastal & Estuarine...historically underrepresented groups. Through such mentoring programs, scientists can build and expand their...

  12. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...28060411. Thomason, Lynn C., et al. "Recombineering: genetic engineering in bacteria using homologous recombination...leaves a “scar” in the chromosome, sometimes interfering with expression of surrounding genes. However...Developed over the past 20 years, Lambda Red recombineering, which uses enzymes domesticated from the Lambda...enzymes, require multiple transformation and plasmid curing steps, and can generate off-target mutations. ...of bacterial toxins to facilitate efficient recombineering (Khetrapal et al., 2015). They created vectors...counter-selection which enabled very efficient recombineering.     We were inspired by this work and sought...16568824. Hmelo, Laura R., et al. "Precision-engineering the Pseudomonas aeruginosa genome with two-step...

  13. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ... IPI and the Springer lab (Hao et al., 2024). In this study, we, along with the Springer lab, reported...stabilizes the extended open integrin conformation. During embryonic development, extended-open integrins ...development, and the shaping of the extracellular matrix. During immune responses, this same conformation encourages...produced from hybridoma antibodies developed in the Springer lab. Some are rabbit immunoglobulin G1 (IgG1) ... to be developed and defined. According to the Springer lab, a highly specific function-blocking antibody...   Targeting integrins in the lab The Ha and Springer labs recently showed RGD-binding integrins demanded...integrins. The Ha lab — in collaboration with the Springer lab, the Jaumouillé lab, the Waterman lab, and...

  14. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...As Addgene believes many of the topics discussed during the symposium will prove incredibly important to... that the main question they wanted to focus on during the symposium was “How many graduate students/post-docs...problems in academic research and potential solutions During the first couple of sessions of the symposium it...In fact it is this very idea that postdocs are acquiring training to become professors that is provided...estimates that postdocs are paid $16.15/h before fringe benefits, less even than the $21/h [on the low ...low end] that grad students are paid before fringe benefits); what they don’t make in wages, they make up...programs as the standard option for scientists by requiring graduate students to pay for part of their tuition...

  15. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...cooking competition featuring a selected ingredient. Anyone who wants to participate brings in a dish that .... The four of us would eat together every day, sharing ideas for the company, but also talking about sports...a culture of openness and trust that comes from caring about your colleagues on a personal level, and ...special occasions. However, employees regularly bring in baked goods and we have a few unique lunchtime... from the software folks and started Hack Days. During Hack Days, teams at Addgene will forgo their regular...website. Free lunch is provided for participants during the Hack Days, and we’ve seen great success at ...university departments to establish, with the goal of fostering better communication between labs. Scientists ...

  16. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...naturally occurring Cas9 diversity via the Integral Microbial Genomes (IMG) database, Harrington et al discovered...are available from Addgene. Happy CRISPRing! References Harrington, Lucas, et al. "A thermostable Cas9...Yes Longevity in Serum?* No Yes Table 1. Comparing SpCas9 and GeoCas9. *For in vitro Cas9 RNP activity...temperatures much warmer than SpCas9 can tolerate. Harrington et al tested GeoCas9’s love of heat in two ways...can introduce genomic edits in mammalian cells. Harrington et al tested GeoCas9’s editing skills by transfecting...had similar levels of editing. See figure 4 in Harrington et al for more examples of GeoCas9’s editing ...sleeve is its increased longevity in human serum. Harrington et al suspected GeoCas9 might have higher stability...

  17. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ... once a week for several months at a time during the spring and fall semesters. Our scheduling was in ...in this situation, considering stopping (temporarily or permanently) the recurring meeting or perhaps ...Enter: the journal club.  A journal club is a recurring meeting amongst scientists that aims to help stay... At my former academic department, we had two recurring journal clubs on Immunology and Microbial Pathogenesis...club? Where will the journal club take place? Preparing for the first meeting There are many important...Addgene bioinformatics journal club! What happens during journal club? There are many common ways to run...meeting using teleconferencing software. Any file sharing service should also serve to share the materials...

  18. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...in more genome engineering technologies? Browse others on Addgene's Genome Engineering Guide. Fire ...libraries expand upon the CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA delivery...protein and cell ageing, as the spectrum shift occurs during Timer protein life due to a form of oxidation (...transcriptomes, as well as further optimization through engineering, Ed Boyden’s lab has identified a variety of ...field of optogenetics. Using a rational protein engineering approach, they designed Opto-RTKs, which activate...flavin cofactors and dimerize on exposure to light, bringing the intracellular kinase domains into contact ...activity levels and limited sensitivity under the stringently low expression conditions necessary to avoid ...

  19. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...to build a bench mentor-mentee relationship and bring your best self to the training experience.  The ... isn’t discussed often enough is the hands on mentoring you receive after you’ve found your mentor, though...lab (and possibly much more, depending on the mentoring style within the research group). The bench mentor-mentee...you don’t do proper preparation and pay attention during training, then you won’t be very proficient afterwards...how to prepare and things to keep an eye out for during training. Preparation before bench work Are there... something then you’ve done previously) you can bring those questions to your training! All of these things... the in-person training component. Documenting during training It’s hard to recall a lot of details, ...

  20. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...purchasing a gift card to use later or ordering take-out) or volunteering to foster a pet, for example. If ...is based in the lab. If you’re unsure what to do during this period, check out these tips and resources...time for exercise. Many studios and gyms are now offering online classes and participating in these is a...class twice a week at Addgene HQ, but now we’re sharing links to online yoga classes. Get outdoors. Taking...ride is a great activity for both exercise and clearing your mind. And because the outdoors is so huge...staying away from others.  Meditate. Meditating during the day is a great way to take a mental break, ...activities like team lunches, a virtual soup lunch, and sharing photos of our pets. #SocialDistancing may send...
Showing: 81 - 100 of 837 results