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We narrowed to 488 results for: Ret;

Showing: 81 - 100 of 488 results

  1. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...Filonov et al., 2013). FRET biosensors were developed based on miRFP760-miRFP720 FRET pair, including Rac1...indicator (Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs were...optogenetics of Rho GTPases enabled by near-infrared FRET. Nat Chem Biol 14, 591-600 (2018). Shemetov, A.A...

  2. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...Evaluating Efficiencies of Dual AAV Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org...(2013) Effective delivery of large genes to the retina by dual AAV vectors. EMBO Mol Med 6:194–211 . https...truncated proteins are safe and effective in the retina of a mouse model of Stargardt disease. Hum Mol ...first time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an AAV transfer...

  3. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated sequences with...cloning and storage of unstable DNA and lentiviral/retroviral vectors. Used by Addgene for pooled library amplification...

  4. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...or PNS transduction, cell type specificity, or retrograde transport in neurons. (b) Genetic cargo can be...typically administered in the lateral tail vein or retro-orbital sinus. IT injections are administered in... Chen et al., 2022 PAL2 CNS: cerebrum; neuroretina Macaques N/A Stanton et al., 2023 AAV-MDV1A...could be used to target endothelial cells in the retina and spinal cord (Krolak et al., 2022). Systemic...macaques. It also effectively transduces the neuroretina. PAL2 detargets the liver and has increased DRG...Zheng, Q., Kozareva, V., Beddow, T., Tobey, I. G., Pacouret, S., Chen, A. T., Chan, Y. A., Ryvkin, D., Gu,...

  5. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...remember that some serotypes can be transported retrogradely and/or anterogradely along the axon. Others,...your gene and could lead to toxicity or trouble interpreting your results, while a low transduction efficiency...Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L. H. (2018). An Alternate...Introduction to AAV Viral Vectors 101: The AAV Retrograde Serotype Viral Vectors 101: Viral Applications...

  6. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ...in real time Proteins involved in endoplasmic reticulum quality control navigate a large and interconnected...factors present unique challenges for study. These secreted signaling proteins regulate vital cell and tissue...subtle changes in co-receptor dynamics, including NanoBRET assays to monitor the binding of fluorescently-labeled..., J., & Hill, S. J. (2021). Use of NanoBiT and NanoBRET to monitor fluorescent VEGF-A binding kinetics...

  7. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...to not have any effect on each other, they have discrete effects on the dependent variable, and those variables...others. Each test has different likelihoods of returning false positives/negatives, but they all generally...numerical value relative to quantiles derived from a theoretical distribution. If your data is normally distributed...130 (2012). 10.1097/SMJ.0b013e3182498ad5 Curran-Everett, Douglas. Explorations in statistics: statistical...

  9. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...Wilson New serotype AAVrg = AAV retrograde (also called AAV2-retro) Looking for more viral vectors? ...marker, a Flp-dependent channelrhodopsin in the retrograde serotype, and a dTomato under the control of ...

  10. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ... user, instead, don’t fret; we have backgrounds for you as well! If you use FRET, you might be interested...Fluorescent Proteins blog topic to learn more about FRET and other techniques using FPs! Otherwise, check...

  11. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...For example, searching the term "breast cancer" returns 40 screens performed in 26 different breast cancer...and other details relevant to screen result interpretation. The analytical method for score assignment...maintain consistency with published screen interpretations and allows users to query screen data without...

  12. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...find this hard so get used to it by making it a concrete goal to say “thank you, job well done” to one ...not the person - intent is usually good. Don’t pretend something went well when it didn’t… but don’t “...compare with your own perception? Tell me your interpretation of my feedback to you. What are We Learning...

  13. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...translated into proteins, or, in the case of retroviruses, reverse transcribe their gRNA into DNA, which...replicative ability. The remaining particle, which retains the protein coat (and envelope), has a “gutted”...they’re derived from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And now that you know what viral...

  14. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...selected to perform the transduction with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired...for the transduction step. Viral particles are secreted into the cellular media, making viral collection...integrate into the host genome (lentivirus and retrovirus) while others are non-integrating (adenovirus...

  16. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...increased yields in adenoviral (Coroadinha, 2006) and retroviral production systems (Shen, 2011). To our knowledge...also improved yields of the AAV2 derivative, rAAV2-retro, but had no effect on AAV5 yields. We further show...Effect of osmotic pressure on the production of retroviral vectors: Enhancement in vector stability." Biotechnology...

  17. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...protein secretion, making harvesting recombinant proteins a cinch. This is because it doesn’t secrete its ...proteins, making the recombinant protein its major secretion. Beyond sheer protein production, K. phaffii is...

  18. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...book! Bonus, Saini's following book Superior: The Return of Race Science is a terrifying and equally good...conclusion? In this series, there is always another secret."     - Marcy Patrick, Associate Director of Quality...force them on everyone I know. I am, of course, pretty biased as the founder, but our book club has become...

  19. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ... that a longer vent time would be sufficient to return the sample back to the original pH. What about...it back in the freezer? We then tested whether returning the tubes to the -80°C freezer after storage on...the pH changes in our solutions are transient and return to normal once the tube is opened and equilibrium...

  20. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...team members have opportunities to develop and stretch then they will be happier and more productive in...the person doing the task will have to learn and stretch a little, but be careful not to go too far. Is...Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual Development...
Showing: 81 - 100 of 488 results