...the question of how much data was necessary and reasonable. PLOS has since clarified their data sharing ... in having exclusive access to data and enjoy reasonable time to make sense of it, with their decision...The real cost of making data accessible A more reasonable concern with the data policy was raised by researchers...how results were obtained and this alone is a reasonable motivation for increasing both data accessibility...published data. The importance of data sharing The reason this topic is important to my lab is three-fold... welcomed. However, expectations must also be reasonable, sensitive to the data type and made as facile...

...with Gibson assembly. While this is a comprehensive mutagenesis method, a drawback to using Gibson assembly...plasmids ready for deep mutational scanning.     3 reasons to be interested in deep mutational scanning Hundreds...rate of mutagenesis, and low throughput.       Gibson Assembly: An easy-to-use technique that joins homologous...especially single basepair deletions. Therefore, Gibson assembly is better suited for creating small, focused... PubMed PMID: 27723752.  4. Fowler, Douglas M., Jason J. Stephany, and Stanley Fields. “Measuring the ...

...2015b) under a CC-BY 4.0 license. Long et al., Nelson et al., and Tabebordbar et al. each chose to try.... used SpCas9 in conjunction with AAV9, whereas Nelson et al. and Tabebordbar et al. used the shorter ...not subject to nonsense-mediated decay. Although Nelson et al. observed only 2% genome editing in one experiment...persist long-term. In accordance with this result, Nelson et al. find that dystrophin restoration is maintained... E, Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal genome editing partially restores ...Dec 31. pii: aad5725. PubMed PMID: 26721683. 3. Nelson CE, Hakim CH, Ousterout DG, Thakore PI, Moreb EA...

...path. Because there is no demand for a favor, the person you ask will be more likely to say yes and more... Informational Interviews are best conducted in person. Perhaps you can offer to buy coffee or lunch at... your interviewee? It is always best to meet in person to develop relationships, but if it is not possible.... Keep in mind that this is a good time to ask personal questions about salary, opportunities for advancement...advancement, challenges and disappointments. These more personal questions can elicit the most valuable insights...

...secrets - during a collaboration, members of Maureen Hanson’s and Rima Menassa's labs made the accidental discovery...created a transgenic GFP-containing organism,” says Hanson, “using a gene encoding one of the unexpectedly...Sattarzadeh A, Saberianfar R, Zipfel WR, Menassa R, Hanson MR. Green to red photoconversion of GFP for protein... PubMed. McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL. A bright and photostable photoconvertible...from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS, Verkhusha VV...

...DNA. Finally, in 1953, Franklin along with James Watson and Francis Crick resolved the DNA double helix...engineering itself is attributed to SciFi writer Jack Williamson who used it in his 1951 novel Dragon’s Island...movies. Rampage (2018), starring Dwayne “The Rock” Johnson, calls out CRISPR as the genetic engineering experiment...Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR (2019) Search-and-replace...Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2018) Publisher Correction: Programmable...:3870–3879 . https://doi.org/10.1093/nar/gkf495 Watson JD, Crick FHC (2003) Reprint: Molecular Structure...

...and Hoess, 1984; Van Duyne, 2001; Christenson Wick and Krook-Magnuson, 2018). Optogenetic FLEx vectors ...PubMed PMID: 11340053. Wick, Zoé Christenson, and Esther Krook-Magnuson. "Specificity, versatility, and...Nature 351.6326 (1991): 489. Hirsch, Matthew L., Sonya J. Wolf, and R. J. Samulski. "Delivering transgenic...PMCID: PMC5111696. Towne, Chris, and Kimberly R. Thompson. "Overview on research and clinical applications...

... should I try research? Personally, I have always been a pretty curious person. I like to ask questions...anyone else in the world knows it—to be the first person to make a discovery. I can’t tell anyone else what...place to begin. Here’s my advice: Reflect on your personal interests While it’s perfectly fine to be unsure... also makes for an experience more tailored to personal interests. Talk to people, ask questions, and ...First-Generation College Students. Social Psychological and Personality Science 11:647–657 . https://doi.org/10.1177/...

...technological change happens really fast,” Thompson says. To do this, Thompson reached out to MIT labs in the Synthetic... classification This inquiry started when Neil Thompson, a research scientist at MIT’s Computer Science...query coverage) that could be determined through comparisons with a reference sequence database. Through a...Kunjapur, Aditya M., Philipp Pfingstag, and Neil C. Thompson. "Gene synthesis allows biologists to source genes...

...proteins without changing excitation wavelength (Salomonnson et al. 2012). They can also be excited efficiently...emission intensities from fluorescent proteins (Salomonnson et al. 2012). Finally, it is worthwhile to test...18718768. PubMed Central PMCID: PMC2569002. 3. Egen, Jackson G., et al. "Macrophage and T cell dynamics during...18261937. PubMed Central PMCID: PMC2390753. 4. Egen, Jackson G., et al. "Intravital imaging reveals limited ...21527931. PubMed Central PMCID: PMC4772972. 11. Salomonnson, Emma, et al. "Cell-based and in vivo spectral...Wolfgang Weninger. "Dendritic cell behaviour in vivo: lessons learned from intravital two-photon microscopy."...

...favourable ratings to the abstracts that affirmed their personal beliefs even though all three texts were otherwise...were exhibiting a phenomenon called “motivated reasoning,” the catchall term for a range of neat little...little tricks our minds perform to avoid cognitive dissonance (Kunda, 1990). More specifically, they were engaging...sources of information they don’t like, looking for reasons to dismiss them. There’s evidence to show that ...nclimate1547 Kunda, Z. (1990). The case for motivated reasoning. Psychological bulletin, 108(3), 480. More resources...

...essential for the structure of paraspeckles. Clemson CM, Hutchinson JN, Sara SA, Ensminger AW, Fox AH, Chess...your favorite biological phenomenon, there’s a reasonable good chance that Addgene can help. Though it ...Fox Elaine Fuchs Kai Ge Robert Kingston Thoru Pederson Phil Sharp Julie Ahringer Alexander Stark Bas..., especially Karen Reddy (@theReddyLab), Thoru Pederson, Joanna Wysocka, Kerstin Bystricky, Ben Martin...

...effectively contribute as part of a team.” As a seasoned educator, Kuldell also knew that the best days...student engagement and joy, rather than a perfect lesson plan or outstanding test scores.  As a result, ...are provided with synthetic biology lab kits and lesson plans adapted from her own MIT curriculum. Drawing...investigate together. Teachers decide which BioBuilder lessons to teach, and how — some choose to run full semester-long...semester-long classes, some pick and choose a few lessons to include each year; others use BioBuilder as ...

..., and substitutions - at the same efficiency as Gibson Assembly and GeneArt cloning - but at a much lower...digested and ligated into an appropriate vector. Gibson Assembly, GeneArt Cloning, and SLIC can all be ...When REPLACR-mutagenesis goes head-to-head with Gibson assembly and GeneArt cloning, the results are favorable...hands!     REPLACR-Mutagenesis Megaprimer PCR Gibson Assembly GeneArt Cloning Types of Mutations Insertions...Made Competent Cells Required Yes No No No Comparison of REPLACR-mutagenesis with other common methods...

...Twitter, LinkedIn and Facebook to see their online personas. Some have online coaching tools that can be very...remotely (by phone, Skype or Google Hangout) but in-person coaching is nice if you can get it.  Because I ...this offers the option of matching with the best person in the group for your needs. Beth Schachter of ... Michelle Capes at Adeptify Me. While based in Madison, Wisconsin she also works remotely and has experience...support for issues of diversity in science.  A great person to contact is Twitter’s @DrQualls. Marquita Qualls...

...genetic tools. Mobile-CRISPRi from the labs of Jason Peters, Oren Rosenberg, and Carol Gross provides...Mobile-CRISPRi is introduced using the bacterial Tn7 transposon system. This system has two parts: a plasmid ...Introducing Mobile-CRISPRi into bacteria. Top: A Tn7 transposon containing the CRISPRi components and a plasmid...methods 2.6 (2005): 443. PubMed PMID: 15908923. Johnson, Christopher M., and Alan D. Grossman. "Integrative...4.2 (2019): 212. PubMed PMID: 30675034. Peters, Jason M., et al. "Enabling genetic analysis of diverse...

...Kostick, K., Torgerson, L., Muñoz, K. A., Hsu, R., Kalwani, L., Sierra-Mercado, D., Robinson, J. O., Outram... rat CNS: AAV PHP.EB vector dose–response and comparison to AAV PHP.B. Gene Therapy, 25(5), 392–400. https...00981-0 Wright, J. F., Le, T., Prado, J., Bahr-Davidson, J., Smith, P. H., Zhen, Z., Sommer, J. M., Pierce... Jason Nasse...

... Jake Watson and Javier García-Nafría from the MRC Laboratory of Molecular Biology. Jake Watson is a postdoctoral...This post was contributed by Jake Watson and Javier García-Nafría from the MRC Laboratory of Molecular...receptor function.     Follow them on Twitter @jakefwatson and @JGarciaNafria.  References 1. Jones, Douglas...PMCID: PMC331480. 3. García-Nafría, Javier, Jake F. Watson, and Ingo H. Greger. "IVA cloning: a single-tube...

...libraries that do just that (Doench et al., 2016, Sanson et al., 2018). These libraries utilize optimized...additional transcription factors to the targeted region (Sanson et al., 2018) . This tracrRNA was combined with...selected per gene and divided into two sets, A and B. Sanson et al. demonstrated that the Calabrese library ... more hits than the SAM library approach in a comparison study screening for vemurafenib resistance in...: 25494202. PubMed Centra PMCID: MC4420636.  5. Sanson, Kendall R., et al. "Up, down, and out: next generation...

...As a person with many roles and responsibilities, I have to think a lot about how to balance those roles...your community’s schools, here are a few tips and lessons I’ve picked up from my recent experiences. STEM...history helped demonstrate that a) I was a real person who used to be a middle schooler himself and b)...back and trying again next year. There are many reasons why you might want to reach out to your school ...tumultuous time in scientific history. Whatever your reasons, let us know how it goes! Acknowledgements: I...