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We narrowed to 484 results for: Vit

Showing: 81 - 100 of 484 results

  1. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...A major limitation of APEX was its low sensitivity. Sensitivity could be increased with higher expression...aggregation. The Ting lab hypothesized that the low sensitivity was due to suboptimal folding or stability. Thus...identified a mutant with significantly increased sensitivity that did not affect the function or efficiency... BirA* molecule that can reassemble to restore activity when fused to two interacting proteins (Schopp...

  2. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...the Mushroom Kingdom. Kravitz Lab 2019 Holiday Door is up and running! @LexKravitz pic.twitter.com/J5bjHQo565...TannevanderWal) December 18, 2019   Holiday door The Kravitz Lab went all out to deck their door. What a fun... December 3, 2019 Hope you’ve enjoyed the festivities in the last month!  ...

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...this post: Glutamate indicators with increased sensitivity and tailored deactivation rates Addgene's own...interneurons Glutamate indicators with increased sensitivity and tailored deactivation rates By Mike LacyGlutamate...significantly improved brightness, kinetics, and sensitivity. iGluSnFR4f is well-suited for imaging rapid ... (2025). Glutamate indicators with increased sensitivity and tailored deactivation rates. bioRxiv, 2025.03.20.643984...). Reprogramming site-specific retrotransposon activity to new DNA sites. Nature. doi: https://doi.org...

  4. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...concentrations are low, pADH1 displays low promoter activity (~20% activity of the strong yeast promoter pTEF). As ...enabling it to bind the promoter and repress pADH1 activity. Researchers have engineered pADH1 variants that...“middle” ADH1 promoter pADH1m, which maintains activity during the late ethanol consumption phase of yeast...is active. In this way, you can interrogate the activity of uncharacterized promoters. Similarly, placing...

  5. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...further rounds of RNAi. This process, known as “transitivity,” calls into question any claims about specificity...specificity of a given RNAi technique. While transitivity enhances the potency of RNAi, it can also increase...transgene, followed by a fluorescence readout of RNAi activity. As a plant researcher, the traditional hpRNA ...Mueller WF, Clauder-Münster S, Eberhard D, Fälth Savitski M, Grandi P, Jakob P, Michon A-M, Sun H, Tessmer... W (2019) Biological plasticity rescues target activity in CRISPR knock outs. Nat Methods 16:1087–1093...

  6. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...enzyme that has been mutated to block its catalytic activity and instead become trapped in a covalent bond ...specificity for their own ligands with minimal cross-reactivity. Other strategies include using engineered ligases...2). If you’re working with purified proteins in vitro, you can even use the same tag for multiple proteins... actin filament assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled...Vivo Covalent TMP-Tag Based on Proximity-Induced Reactivity. ACS Chemical Biology, 4(7), 547–556. https:/...

  7. Addgene Stands with the Scientific Community

    Type
    Blog Post
    Published
    April 23, 2025, 1:30 p.m.
    ...agility as a nonprofit resource can prove to be vital during this period of constrained funding. Addgene... even more valuable as labs look to maximize productivity and efficiency with fewer resources. Fostering...community has demonstrated remarkable resilience and creativity in the face of funding challenges. By strengthening...benefit of everyone. We Want to Hear from You We invite researchers to share how these funding changes ...

  8. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...of plasmid vectors and/or rely on cumbersome in vitro synthesis of single guide RNAs (sgRNAs), are time-consuming...tracrRNA system is much more potent than the in vitro-transcribed single guide RNA (IVT sgRNA) approach...Direct comparison of Cas9 RNPs prepared with an in vitro-transcribed (IVT) sgRNA versus synthetic cr:tracrRNA...efficiently and serves as a good control for both the activity of CRISPR reagents and ICC antibody specificity...of the crRNA (aim for 40-60% GC content) crRNA activity Local chromatin structure (although we could successfully...

  9. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ...especially when done in vivo. There are also sensitivity issues when attempting to characterize mutations... the need for a faster and higher-throughput in vitro approach. Using data from The Cancer Genome Atlas...them had not yet been functionally validated. In vitro assay allows first-time functional annotation of...collection to screen individual perturbations in vitro using Ba/F3 and MCF10A cellular models. Ba/F3 and...

  10. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ... H+ specific response cytoplasmic retention insensitivity to other ions In BRET, A mutant Renilla luciferase...mutagenized and screened for speed and voltage sensitivity. This hierarchical screen led to the generation...QuasAr2 possess mutations for improved voltage sensitivity and speed, and an endoplasmic reticulum export...construct, named CheRiff, exhibits high light sensitivity and fast kinetics. By combining both the voltage...produced luciferase is truncated and lacks enzymatic activity. The Luc plasmid (intronless) is useful for counter-screening...vector that enables protein expression in any in vitro translation system. The gene set was carefully chosen...the study of promoters with either weak or high activities (Bert, et al. 2000). To design pXPG, the authors...

  11. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...been removed/mutated to neutralize the nuclease activity. For strains that still carry this gene, there... purification protocol to remove endonucleases activity. For these reasons choosing the correct solution...any divalent cations like Mg2+, preventing the activity of DNase. While this is very important to preserve...temperature or at 4°C. Storing DNA: temperature and longevity You should consider a couple of more things when...

  12. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...this episode of Hot Plasmids! Light controls the activity of a microtubule-associated protein to regulate...quantification of base-editing and Cas9 cleavage activity Article contributed by Angela Kaczmarczyk  Listen...fluorescence is activated when APOBEC-Cas9n-UGI-mediated activity edits out a frame-shift mutation within the mCherry... utility in quantifying multiple gene editing activities. Click here to find the ACE reporter plasmid...

  13. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ... a voltage sensor AAV Historically, electrical activity in the brain was measured with implanted electrodes...reporter allows a way to monitor neuronal electrical activity through imaging. Since it’s illuminated by red...fluorescence and resting fluorescence. It is useful for activity imaging of large populations of densely labeled...fluorescence and resting fluorescence.  It is useful for activity imaging of large populations of densely-labeled...

  14. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...contributed by Aliyah Weinstein Sparse Predictive Activity through Recombinase Competition (SPARC) is a fully...788679 A destabilized GFP reporter for calcineurin activity in yeast Article contributed by Leah Schwiesow...propidium iodine (PI), allowing for calcineurin activity measurements in stressed or aging cell populations...al., 2020. Find the GFP reporter calcineurin activity here Diessl et al., Microbial Cell, 2020. https...

  15. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...motivated by a particular interest in monitoring the activity and biochemistry of living neurons in an effort... calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes...approach he and his colleagues took to optimize sensitivities can now be applied to dozens of other published...concentrations than calcium, requiring greater sensitivity. He and his colleagues came up with a solution...

  16. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ... expertise, publications, and plan for future activities; including the interest of the greater scientific...one that you can pay for, but one that you are invited to join (selected due to expertise in field). Publicity...journal editors to request to be a reviewer. Proactivity is very important in this case.  You can still...it as documentary evidence for your case – the invitation to be a peer reviewer needs to be addressed to...least 3 full-time people engaged in researcher activities. The EB-1B requires that you provide two out ...

  17. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...increase activity, enhance binding between the template and primer binding site, increase processivity, or ...prime editor sequence to improve expression and activity In addition to the numerous innovations that streamline...mutations in Cas9 previously shown to improve nuclease activity (Chen et al., 2021). In a system as complex as...R221K N394K Cas9 mutations Improve Cas9 nuclease activity ~ ~ ~ ~ ✓ ✓ ✓ Evolved reverse...

  18. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...study tested AAV infectivity after a range of pH exposures. They showed that AAV infectivity was not altered...alter the infectivity of the virus. However, there was a substantial reduction in infectivity after 3 weeks...

  19. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...WYL1 increases RspCas13d and EsCas13d cleavage activity The majority of Type VI-D loci contain accessory... by RspCas13d. RspWYL1 also increased EsCas13d activity, indicating that WYL domain-containing proteins...proteins may be broader regulators of Cas13d activity. This property makes WYL proteins an intriguing counterpart...anti-CRISPR proteins that negatively modulate the activity of Cas enzymes, some of which are also functional...

  20. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...polymerase, which has 3′→5′ exonuclease activity. T4’s polymerase activity can be thwarted if no dNTPs are available...to polymerize, allowing the slower exonuclease activity to proceed. By including only one type of dNTP... reading Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Li MZ, Elledge...
Showing: 81 - 100 of 484 results