...see the new map in action). Read on to learn more about the improvements we’ve implemented and how they’...more functional display, you can start thinking about your next cloning experiment early on. Beyond these... to to download the static image file (PNG, see above) for your own reference or to paste directly into...identified a plasmid that you want to learn more about, updates to our sequence analyzer will allow you...the features shown in the static maps discussed above, but also have interactive functionalities. You ...enzyme, primer, or ORF to learn more information about it. For example, if you hover over the ampicillin...guide A quick guide to all of the tabs listed above and their functions. We hope that these updates...

...designed (listed above) worked well and I got clear bands using the conditions above. 2. Design your primers...essential for experiments but that nobody talks about. After months of troubleshooting, those people who...details. The entire process from start to finish took about 1 month. Cas9 delivery Overview: Before any ...is a loading control. Pro-Tips: 1. It’s all about the MOI. In retrospect I used a way too high Cas9...original publication they see GFP expression in about 20% of their Cas9-expressing cells. So, even when... gene has a weird mutation that you didn’t know about (which could affect the ability of the gRNA to bind...engineering has come. For comparison, in 2007 it took about 3 months to knockout a single allele in a human ...

...the Addgene Blog Learn More about Homology Directed Repair Learn More about Non-Homologous End Joining .... For a C-terminal tag, as shown in the example above, we inserted a linker and GFP tag at the end of ... and 1kb of homologous DNA flanking both sides (about 2.7kb total)—is inserted into a plasmid backbone... Plasmids, plasmids everywhere To find out more about gene edited hiPS cell lines generated using the ...line. The editing strategy and design described above can be used to create similar donor plasmids for...microhomology-mediated end-joining (MMEJ). To find out more about gene edited hiPS cell lines generated using these...

...introduction to my area, a line about the research I will be doing, and a line about what I hope to discover....themselves many times and will have useful advice about what reviewers in your area are looking for. Remember...One way to add originality to your work is to collaborate with specialists in different fields that can...the field. 3. Highlight your network and your collaborators It’s a good idea to move around to different...outcomes can include poster presentations, talks about your work, or even the successful use of your preliminary...quality application. Even following this advice, only about 50% of my grant applications have been successful...on your grant application to your peers and collaborators can also help you get some critical insight ...

...Addgene Blog Learn about Synthetic Biology with BioBuilder Use Addgene Videos to Learn about Basic Lab Techniques...species, but these studies generally leave questions about gene function wide open. What if two seemingly similar...been using yeast models (3, 4) to answer questions about the evolution of the genes involved in methionine...question, the plasmids are also used to teach students about restriction mapping and western blotting. Students...communicating science to the public. You can find out more about Clare at the Biology Dept. site.  References 1. ...PMC4596657. 5. Thomas, D., & Surdin-Kerjan, Y. Metabolism of sulfur amino acids in Saccharomyces cerevisiae...Functional genomics research in an introductory laboratory course. CBE-Life Sci. Educ. 17, ar1. (2018). ...

...more about Dr. Kwapata’s current work and thoughts on the need for more scientific collaboration across... connecting us with him. Learn more about our recent collaboration with Seeding Labs here. Special thanks...received an award from nonprofit and Addgene collaborator, Seeding Labs, for lab equipment that will help...Addgene Podcast.     Additional Resources Learn about Seeding Lab's Work with Lilongwe Univeristy of Agriculture...How You Can Contribute to the Addgene Blog Learn about Addgene Plasmid Donations for Rare Disease Research...

...Blog Learn about Engineered Cas9 PAM Variants Read about CRISPR Base Editing Learn about Homology Directed...editing specificity. We’re excited to learn more about xCas9 - here’s what we know so far! Why do we ...point mutations, also has a strict editing window about 15 nt upstream of the PAM. Even if there’s a PAM...in mammalian cells, finding xCas9 3.7 displayed about the same editing rate as SpCas9 at an NGG PAM. When...are exciting, there’s a lot we still don’t know about xCas9. “I’m not 100% sure xCas9 is going to be flat...Find CRISPR Plasmids for Mammalian Systems Learn about CRISPR History ...

...negotiate with friends, partners, and/or children about everything from what you’re going to eat for dinner...Negotiation as a zero sum game Some people think about negotiation as a zero sum game - that is, if I get... three examples depend on many specific details about the individuals, each example provides a scenario... difficult because of the need to be thoughtful about what is most important to each person. It can especially...of us associate with negotiating (check out more about conflict resolution). How to develop negotiation...before, during, or after a negotiation to think about the situation, your reaction, and the outcome. How... able to take my vacation? I want to learn more about a different area of the company. How can I negotiate...

...record readership with over 75,000 views. Changing Labor Laws Bring Increased Postdoc Wages Our most popular... for anyone considering a postdoc. In “Changing Labor Laws Bring Increased Postdoc Wages,” Future of Research...thorough yet concise rundown of how recently enacted labor laws will likely increase postdoc earnings in the...changes quickly and harmlessly. Learn much more about the laws and their possible repercussions in Gary...This post leads readers to our latest podcast all about graduate student life and how establishing a thriving...to Use Reddit for Science Communication Changing Labor Laws Bring Increased Postdoc Wages I hope you all... labor laws...

...wonderful tangents. Some groups asked about vaccines, others were curious about the agricultural repercussions...roles and responsibilities, I have to think a lot about how to balance those roles. I’m a father of three...community. This past year, I spent some time thinking about how I could combine all of these roles by reaching...guest presentations. If you’re a scientist thinking about reaching out to your community’s schools, here are...accountants running our math tables!  Be realistic about what you can teach  5-10 year olds in a single evening...DNA, protein, plasmid, gene, etc. Though I talked about CRISPR’s molecular mechanism, I was careful to keep... I gave my presentation five times to groups of about 50 students each, and each group went on its own...

...think about. Choosing a topic and gauging interest The first thing to do if you’ve thought about starting... discussions. Beyond the excitement of learning about new cutting edge science, researchers of all varieties...help stay current on new and emerging work by collaboratively discussing recent publications in a specific...up. At Addgene, we wanted to think more closely about the wealth of high-throughput sequencing (HTS) data...the paper.  Before the first journal club, think about the meeting itself A journal club should aim to ...published science. They also serve as a way to learn about work outside of one's own field in a structured ...hope that with some of the information described above you feel empowered to start your own journal club...

...created The Addictive Brain to spread awareness about how scientists utilize tax-payer money and government...Here, Sadangi and his team answer a few questions about The Addictive Brain and science communication for...science communication? At The Addictive Brain, we collaborate with scientists from around the world and help...numerous feedbacks from our non-scientist readers about how our articles have helped them understand different...at The Addictive Brain? This year, we plan to collaborate more with physicists, chemists, and mathematicians...having to extensively use Google search. Be careful about hidden connotations behind a word. Non-scientists...Sign up to receive our science career guide Learn about different careers in science communication Resources...

...for gene manipulation together with information about the genome are powerful resources for investigating...zebrafish genome was published in 2013, revealing that about 70% of human genes have a counterpart in zebrafish...networks that regulate processes from development to metabolism to fertility. Several zebrafish-specific gene...gene expression databases that provide information about when and where genes are active in developing embryos...genome sequences become available, new hypotheses about specific genetic interactions arise and tools to...genome, their long three-month generation time and labor-intensive maintenance compared to invertebrate model...Patrick Müller’s group at the FriedrichMiescher Laboratory of the Max Planck Society in Tübingen, Germany...

...To learn more about the STIN's podcast, email [email protected]    For more info about starting your...evens where scientists can grab a beer and chat about their work at a local bar. It doesn't have to be...1: Introduction - Vini and Amy tell us a little about themselves. Chapter 2: What is Science in the News...science communicators. In this chapter we learn about some of their events - science seminars, science...the lab and working with SITN. She points to collaboration and delegating as keys achieving success. Chapter...your own SciComm organization and to learn about some of SITN's other initiatives, email [email protected]...

...blog Read about COVID-19 research on the blog Visit our guide for external collaborations Resources ...scientific sharing. Already we’ve seen articles about COVID-19 on preprint servers and in journals, and...vary, everyone we spoke with sees sharing and collaboration as the best way that the scientific community...useful resources during this time. Facilitate collaborations within the scientific community The team behind... Canada is a database that provides information about reagents, funding and volunteer opportunities, protocols...CCRP2) at Rutgers University not only supports collaborations at Rutgers, but also lists data and experimental...

... passionate about. Check out my previous post if you're looking for more information about the benefits...every spurious result under the sun. Academia is about convincing results, and nothing is more convincing...is your passion? Even if no one else is excited about it, distinguish yourself with this passion. “Love...presentations and of course, networking and collaborating.” Getting out of the lab also “opens the student's...emphasizes the importance of passion, hard work, collaboration, and staying honest with personal goals. Stay...George Church for answering our burning questions about how they manage and motivate their labs! Update...

...databases mentioned above or, even better, first-hand knowledge from laboratories employing CRISPR on ... CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over 90% in ...targetable NGG PAM sites whereas the human genome has about 300 million. With such a sparsely targetable genome...worse by an ever increasing marketplace. Since 2013 about 11 tools have been added every year.   Breaking ...databases are still young and will require a collaborative effort from the community in order to succeed...screen shot and overview of the table can be found above). These categories are further discussed below (.... For this reason I generally recommend that laboratories that have already attempted several CRISPR experiments...

...allow you to collaborate within the platform and avoid countless emails. Important collaborative functionalities...of the software. We identified that when talking about ELN costs and limited budgets, “a large percentage...Besides that, academic institutions tend to think about the long term, so they need a solution for which...institution. In the study, respondents were also asked about how important ease of use is for them (if they are...or partners to access your data? If you are collaborating with other labs or clients, you might need share...comments, added files, notes etc. Detailed records about every action performed in the lab (user, date, time...Additional Resources on the Addgene Blog Learn about more tools for reproducibility 5 great apps for ...

...rely on what I know about those particular teams at that given time. I think about how previous iterations...disagreements. In fact, conflict resolution isn’t just about resolving conflicts between other people, but resolving...normally get along well with each other disagree about a specific decision, direction, or goal. Resolving...of lapsed communication. Making some assumptions about how something should be communicated is necessary...confusion around... Ownership: Misunderstandings about who is responsible for what tasks or projects is...conflicts.  Transitioning a conflict into a collaboration may not be the easiest resolution to accomplish...cooperation: avoiding, competing, accommodating, collaborating, and compromising. Though there may be a time...

...Science Communication blog series, you’ll learn about Caitlin Runne-Janczy, a Product Development Manager...G-proteins at the time. This program takes what you know about it [your area of research], and teaches you how ... hustle on the side. “I actually stumbled upon, about 6 months in, this opportunity to do scientific editing....” But Caitlin’s persistence paid off: “I spent about 6 months just editing and searching for a job and...Additional Resources on the Addgene Blog Learn more about alternative science careers here Be like Caitlin...just for good measure, here’s a few other posts about developing transferable skills while in grad school...school Resources on Addgene.org Learn more about careers at Addgene ...