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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 235 results for: bon;

Showing: 81 - 100 of 235 results

  1. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...tagging the kinesins KIF5A and KIF5B as well as backbones for generating other SNAPf-, CLIPf-, and CLAP-...biosensor in optimal proportions from a vector backbone that allows lentiviral packaging as a single payload...

  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...Thankfully, this is a hot topic with many resources! Bondeson et al. (2022) reviews all the tags discussed here...choice!   References and Resources References Bondeson, D.P., Mullin-Bernstein, Z., Oliver, S. et al....

  3. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...the desired mutation and excision of the vector backbone. To do this, rhamnose is added which induces expression...point will be those that have excised the vector backbone. Vectors pTOX4, pTOX5, and pTOX6 express the ...

  4. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... based on the copy number of the empty plasmid backbone or a researcher’s personal observations. If you..., buffering agents, and/or supplying additional carbon sources such as glycerol or glucose (in moderation...

  5. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...generating transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified Cas9 protein and in vitro...Topic Page Learn about CRISPR Delivery Using Ribonucleoproteins (RNPs) Read Tips for a 1st Time CRISPR User...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...making the ribosome skip the synthesis of a peptide bond at the C-terminus of a 2A element, leading to separation...insert the whole multicistronic cassette into a backbone as a single unit. Although it is recommended to...

  7. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm. Microbial Cell Factories...-White Screening Choosing Your Perfect Plasmid Backbone  ...

  8. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...plasmid backbone that was designed specifically for transient expression (find some useful backbones here...

  9. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...protocol!  First, the authors created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly...consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

  10. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...compare their options for tools such as empty backbones, lentiviral packaging systems, and luciferase ...Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids ...

  11. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...an option to transduce cells directly with ribonucleoprotein cargo. eVLPs contain no genetic material, ...particles for transient delivery of prime editor ribonucleoprotein complexes in vivo. Nature Biotechnology, 10.1038...

  12. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...interest, painstakingly cloned into the perfect empty backbone, made sure to add the right tags to your gene,...metabolism pathway Inability to utilize arabinose as a carbon source leuB β-isopropyl malate dehydrogenase ...

  13. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...uses the direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates...//doi.org/10.1093/nar/gku289 Chen C, Fenk LA, de Bono M (2013) Efficient genome editing in Caenorhabditis...

  14. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...plays some role, and mismatches with more hydrogen bonds are more readily ligated than those with few. For...many ligases, G:T mismatches, with two hydrogen bonds and a base-pair size nearly indistinguishable from...

  15. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...activity and instead become trapped in a covalent bond with its substrate. Then, all it takes is a bit ...find self-labeling fusion proteins or cloning backbones to study your gene of interest with these tools...

  17. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...for the target:probe/primer bonds to be more stable than the hairpin bond.  Alternatively, chemistries...

  18. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...domain that promotes the formation of an isopeptide bond between 2 peptide tags, SpyTag and KTag. The group...plasmids for a given species are based on the same backbone to permit expression levels to be directly compared...

  20. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ... over to our YouTube channel and watch it now! (Bonus: Tim kindly offered to finishing answering all submitted...
Showing: 81 - 100 of 235 results