We narrowed to 84 results for: cas9 expression construct

...(tTA, also expressed by your transfer vector) binds to the TRE and activates the expression of your gene...the different vectors used, lentiviral vector constructs have proven very useful due to their ability ...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral...generation, the HIV Tat gene (previously used to drive expression from the LTRs) has been removed, Rev (which facilitates... facilitates nuclear export) is expressed from a separate plasmid, and the promoter of the 5’LTR has been...Stable integration into host genome and stable expression of the transgene   Transfer plasmids The transfer...a simple set-up where the gene of interest is expressed together with a marker (Fluorescent protein or...

... vitro, and can be used for measuring protein expression or localization in cells, or even single-particle...background signal (especially for some older split-GFP constructs or certain cell environments). Also consider ...Split-horseradish peroxidase (Alice Ting Lab 2016)  Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015...

...different versions of dCas9. Following integration, Mobile-CRISPRi is stably expressed with no selection ... system. This system has two parts: a plasmid expressing the Tn7 transposition genes (tnsA, tnsB, tnsC...efficiency, as measured by knockdown of a constitutely expressed RFP reporter, was variable across bacterial strains... a proof of principle, Peters and colleagues constructed a 40-member library targeting 20 genes of Enterobacter...induction of CRISPRi knockdown. As expected, bacteria expressing guides that target essential genes were less ...A Tn7-based broad-range bacterial cloning and expression system." Nature methods 2.6 (2005): 443. PubMed...antibiotic resistance selection markers, gRNAs, and dCas9 proteins. Second, it can be introduced into a broader...

...al., 2024). In MagIC-cryo-EM, target proteins (expressed as a GFP fusion in this proof-of-concept study...showed that AAV-delivered CHARM could silence the expression of prion protein in mouse brains for at least...gene product; it simply turns the target protein expression off. Plus, DNA methylation is a long-lasting ...-silencing CHARM variants that stop their own expression after silencing their target — potentially improving...by targeting marker proteins that are uniquely expressed in a given cell type. For example, glial fibrillary...GFAP) is a glia-specific intermediate filament expressed in astrocytes throughout the brain, making it ...Fourier-shell correlation curve (D), and the reconstructed 3D map with 3.1 Å global resolution (E). Figure...