Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 170 results for: cas9 expression plasmid

Showing: 81 - 100 of 170 results

  1. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...pool of lentiviral plasmids. Multiple rounds of digestion and ligation result in plasmid libraries containing...multiplexed gRNA expression (Wong et al., 2016), in addition to the combinatorial expression of other genetic...and ligation steps to build barcoded lentiviral plasmids containing one or more gRNAs. As each ligation...containing combinations of different gRNAs in each plasmid. Each gRNA combination can be tracked and quantitatively...activity and minimize off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180...transcriptional activation by an engineered CRISPR-Cas9 complex." Nature (2014). PubMed PMID: 25494202. ...PMC4559080. 8. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout screening in human cells." Science 343.6166...

  2. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ... to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab. Figure 2: Cas9 RNP complexed...genes more effectively than Cas9 complexed with sgRNA. Direct comparison of Cas9 RNPs prepared with an in...Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease,...on the Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein Tags Fluorescent...Addgene.org Browse CRISPR plasmids Check out our CRISPR guide pages Find ready-to-use Cas9 lentivirus ...target site. With the widespread adoption of CRISPR/Cas9 technology and rapid improvements in the field, ...from many pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis...

  3. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...recruit transcription factors to increase gene expression. CRISPRa methods vary in their transcriptional...chains, which are relatively large and are not expressed consistently throughout cells. VPR Description...Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic...J, Collins JJ, Church GM (2015) Highly efficient Cas9-mediated transcriptional programming. Nat Methods...Perrimon N, Collins JJ, Church G (2016) Comparison of Cas9 activators in multiple species. Nat Methods 13:563...transcriptional activation by an engineered CRISPR-Cas9 complex. Nature 517:583–588 . https://doi.org/10.1038...Gersbach CA (2013) RNA-guided gene activation by CRISPR-Cas9–based transcription factors. Nat Methods 10:973–...

  4. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.  ...2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative...Blainey’s lab has developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi...using selection by Puromycin, Zeocin®, or nuclear expression of BFP2.     Figure 1: CROPseq-multi ... over the original CROPseq. Find CROPseq-multi plasmids here Walton, R.T., Qin, Y., Blainey, P.C. (2024...Anzalone et al., 2019, including modifications to the Cas9 or reverse transcriptase enzymes, inhibition of ...end of the pegRNA, which is not well protected by Cas9. This is similar to the proposed mechanism for engineered...

  5. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...Communications Find the Cas12b plasmids  Plant base editing with a Cas9-adenosine deaminase fusion Caixia... paper in Science Find the anti-CRISPR expression plasmids CRISPR-based method for cell lineage tracing...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ... - this time using the Staphylococcus aureus Cas9 (SaCas9). This AAV-based genome editing system is regulated...Molecular Neuroscience Find the AAV-based SaCas9 genome editing plasmids String assembly gRNA cloning (STAgR...tRNA adenosine deaminase that is fused to a nicking Cas9. This base editor can be used for A•T to G•C edits...

  6. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ...effects of gRNA and Cas9 expression, they fail to account for the effects of Cas9-induced dsDNA breaks... that either stably expressed Cas9 or that had been lentiviral infected with Cas9. Validating the Library...Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved.... Unanswered questions about genome-wide CRISPR/Cas9 screen design Are non-targeting guides an appropriate...single-guide studies? Many strategies exist for reducing Cas9 off-target effects, but most studies have focused...previously identified essential genes while previous Cas9 and shRNA library screens only identified  60% of...screen. This toxicity was used a proxy for detecting Cas9 cutting. Safe targeting guides better control for...

  7. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    Published
    July 28, 2015, 2:30 p.m.
    ...of the second plasmid can be done in one day in one assembly reaction. This second plasmid saves you a ...alerts when plasmids become available.  We’ve also had some requests for variations on the plasmids we’ve developed...and Myers for speaking with us! Find the Plasmids: Plasmids for protein tagging with CRISPR Additional...efficiently, they’ve recently adapted the CRISPR/Cas9 system for protein tagging. We spoke with Mendenhall... available at Addgene. For more details on the plasmids that are currently available along with detailed...several projects and this is one related to gene expression. One goal of the ENCODE project is to look at...system, what we are doing is adding in a second plasmid to target an epitope tag onto the transcription...

  8. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...protein can then be co-expressed in essentially any transgenic zebrafish line expressing a GFP-tagged version...display increased neuronal expression in the brain while having decreased expression in the liver after systemic...only AAV.CAP-B10 causes brain wide expression, with no liver expression, after retro-orbital sinus vein ...mutations. These plasmids contain catalytically inactive dCas9 and microbial SSAPs (dCas9-SSAP) which insert...Increased neuronal expression using Cre-mediated AAV Targeted Evolution method (M-CREATE) dCas9-SSAP with reduced...

  9. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...off-target editing based on Cas9 off-target activity.  Find CRISPR base editing plasmids at Addgene Improving..., a base editor containing high fidelity Cas9 variant HF-Cas9 (Rees et al., 2017). HF-BE3 showed 37-fold...other methods. In a head-to-head comparison with Cas9, Cas9 modified 9/12 known off-targets with a 14% indel...individual Cas9. They also mutagenized the cytidine deaminase portion of the base editor to create SpCas9 base...et al., 2020 SpRY Cas9, available fused to ABEmax(7.10) or to BE4max   Evolved SpCas9 with NRN or NYN PAM... 1.1%. These numbers are a vast improvement over Cas9-mediated HDR for the loci tested, where average ...using a cytidine deaminase from sea lamprey fused to Cas9 nickase (Nishida et al., 2016). Target-AID acts ...

  10. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...This PITCh plasmic is cotransfected with a plasmid carrying Cas9, the PITCh-gRNA, and the locus-specifc gRNA...containing Cas9 and the PITCh-gRNA. Step 3: Contransfect the PITCh vector with the vector carrying Cas9 and ... The ready-made PITCh plasmids available from Addgene are perfect for expressing GFP from a given promoter...CRISPR-Cas9 with the PITCh systems.” Nat Protoc. 11(1): 118-33. PMID: 26678082. Find the plasmids from ...Guide Pages Find CRISPR Plasmids for Your Experiments Deposit Your New Plasmid Tools with Us  ...Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell lines can be created in about... knock-ins, some labs have used NHEJ for whole plasmid integration; however, since NHEJ is error-prone...

  11. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...the ability to distribute plasmids with the piggyBac™ transposon. These plasmids, when combined with a source... minimize expression levels of your gene of interest, as needed. Controlling the expression levels of ...useful resource. While Addgene cannot distribute plasmids with the piggyBac™ transposase itself, please ...genome fully intact, while, with a standard DNA plasmid transfection, a double-stranded break may occur...may be particularly useful when the gene being expressed has threshold effects, such as with signaling ... can also mitigate the deleterious effects of expression of certain genes, such as those that have negative...the development of site-specific nucleases (CRISPR/Cas9, TALENs, and ZFNs). However, several challenges ...

  12. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...specifically, they’ve proposed to use plasmids with modified nickase Cas9 protein in order to target the specific...studying four of those rare diseases will receive plasmids from Addgene worth a total of $5000.  Lowe Syndrome...involving the primary cilia. They’ll put a variety of plasmids to work to explore the effect of various OCRL1...pluripotent stem cell (iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit mutations into...looking at a bunch of different strategies with CRISPR-Cas9. There are so many different variants and strategies...there. This will help us acquire a lot of those plasmids so that we can try many different things in parallel..., will use GFP-ATP1A3, FLAG-ATP1A3, and ATP1B3 plasmids to explore the functional differences among mutations...

  13. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ... A plasmid in the host bacterium expressing dCas9 A plasmid in the host bacterium that expresses gene... which rely on the expression of gene III to create infectious particles. A plasmid-based genetic circuit...base-editing activity. A plasmid-based genetic circuit in the bacteria links the expression of gene III to base...compatible with all Cas9 and Cas12 homologs that the researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a...the DNA. ABEs are somewhat slow and need a strong Cas9 domain to “hold on” long enough to the DNA to induce...circuit in the bacteria links the expression of gene III to base editor activity and editing speed and consists... control of a T7 RNA polymerase and cannot be expressed until the base editor corrects early stop codons...

  14. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...alternative Cas9, from Streptococcus aureus, is as effective at editing mouse embryos as Cas9 from S. pyogenes...create a mouse model, the gRNA, Cas9, and donor oligonucleotide or plasmid components are brought together... Find CRISPR Plasmids for use in Mammals Check out Our CRISPR Guide Pages Find Plasmids for Mouse Targeting...guest bloggers, Wenning Qin and Haoyi Wang. CRISPR/Cas9 is revolutionizing the mouse gene-targeting field...ever to manipulate the mouse genome using CRISPR/Cas9. Similar to the human genome, the mouse genome ...robust in performance. In its natural setting, CRISPR-Cas9 is an acquired immune system in bacteria and archaea...) of about 125 nt that specifies the target, the Cas9 endonuclease that creates the DNA double-strand ...

  15. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...co-conversion markers. Expanding beyond Cas9 Since the development of CRISPR/Cas9 editing methods, other Cas proteins...and yeast cells using modified Cas9 proteins to regulate gene expression (CRISPRi/CRISPRa), visualize specific...University of California, Santa Cruz. Emerging CRISPR/Cas9 editing technologies have transformed the palette...regulation during developmental processes, CRISPR/Cas9 allows us to delete sequences and introduce mutations...remain in the edited animal. Development of CRISPR/Cas9 editing strategies in C. elegans As brilliantly ...step as simple as heat-shocking to induce the expression of a Cre recombinase contained within the knock-in...reported in 2017 uses the direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair...

  16. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...similarities and differences to Cas9. DNA targeting Cas enzymes Cas9 – The genome engineering workhorse...CRISPR’ without shortly thereafter hearing ‘Cas9’. The Cas9 nuclease is a genome engineering powerhouse...making Cas9 an adaptable choice for many genome engineering endeavors. For more information on Cas9 variants...same mechanism as traditional spCas9. If you are looking to modify RNA, Cas9 just won’t cut it – literally...CRISPR beyond spCas9 Cas proteins More resources on the Addgene Website CRISPR Plasmids and Resources...We are all oh so familiar with the nuclease Cas9, but how does it stack up to the new Cas enzymes on ...proceed to generate a blunt DNA double strand break. Cas9 has been studied for genome engineering purposes...

  17. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...nicking Cas9. A guide directs Cas9 to a site of interest, where it nicks its target DNA (left). Cas9 dissociates...specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources on Addgene....error prone PolI3M that’s fused to a nicking Cas9 (nCas9). Similar to mutagenesis with error prone PCR... a user-defined genomic region. EvolvR, a CRISPR-Cas9 based targeted mutagenesis method developed by the...Require stringent bacterial growth conditions. Expression of an error prone polymerase I (PolI3M) is capable... targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo...Browse our CRISPR guide pages Find bacterial CRISPR plasmids ...

  18. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...control expression of their genes of interest. While recombinases are powerful gene expression tools in...changes in a protein.    Cells expressing an RFP reporter plasmid for recombinase activity were exposed...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...head over to our CRISPR Plasmids and Resources page.  CDDR (CRISPR–Cas9-based Dual-fluorescent DSB Repair...linked SaCas9 ABE variants. It's also one of the smallest AAV-deliverable ABE.  Find the plasmid here ...

  19. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head... Get the plasmids at Addgene Sc++ is a codon-optimized, engineered Streptococcus canis Cas9 nuclease ...Applications (CHyMErA) Knockout Libraries express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. ...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...excitatory and inhibitory synapses, respectively. Once expressed in the neuron, the FingRs bind to the endogenous...

  20. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...Tight control of toxin expression is absolutely necessary, because leaky expression of toxin could select...Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria." Plasmid 51.3 (2004): 246-255. PubMed...introduce a mutation in the chromosome is to disrupt expression of a gene with an antibiotic resistance cassette...in the chromosome, sometimes interfering with expression of surrounding genes. However, there are ways...(Thomason et al., 2014). In recent years, CRISPR-Cas9 technology has also aided in the creation of scarless...exogenous enzymes, require multiple transformation and plasmid curing steps, and can generate off-target mutations...a mutated allele introduced from a conjugative plasmid replaces the chromosomal copy. This is a versatile...
Showing: 81 - 100 of 170 results