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We narrowed to 119 results for: control GFP

Showing: 81 - 100 of 119 results

  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ... under the control of the cI857 temperature-sensitive repressor. The temperature-controlled expression..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They... at a dose and duration sufficient to kill all control cells and surviving “mutant” colonies were deep...GTPases involved in cell signaling pathways that control many cellular processes such as differentiation...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ...gRNA backbone plasmids with Addgene. Additional control plasmids for the TKO library will be available ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...

  2. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...both purified CPP-tagged protein and an untagged control could enter cultured cells and activate expression...Neuronal stem cells transduced with iTOP resulting in GFP expression. Image courtesy of Diego D'Astolfo. ...

  3. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ... state-of-the-art NIR FPs can be used similar to GFP-like probes by supplying a single gene to cells. ...2018). NIR FPs can be combined with blue-light controlled optogenetics, such as channelrhodopsins and tools...crosstalk-free three-color imaging with green and red GFP-like FPs (Shcherbakova et al., 2016), as well as ...for miRFPnanos, 35 kDa for miRFPs, and 27 kDa for EGFP) and a possibility to be used as internal tags due... normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0 was...

  5. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG). There...like to isolate a minimum of five. You have no control over how many insertions occurred. Having too many...segregate 3:1 in the T2 generation. You also have no control over where your construct will be inserted. If ...

  6. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...most important ways to spatially and temporally control gene expression, especially in in vivo models, ...-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem...functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT recombinase. When placed under different.... The use of AAV provides spatial and temporal control without the need for germline modification, and... and sequence overlap (coral mOrange2, jellyfish EGFP, and sea anemone mKate2.) They then successfully... is retained in Brainbow-3.0, but with mOrange2, EGFP and mKate2 as the fluorophores; mOrange2 is the ...

  7. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...vectors in the “controls” category, which includes things like viral vectors that deliver GFP.   Figure...Patrick, M., Guerin, K., & Fan, M. (2023). Quality control for Adeno-associated viral vector production. Neuromethods...

  8. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...ones? Many of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free...fluorescent state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure...

  9. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...needed and when is it not? What are the proper controls needed to pressure-test your FRET assay to make...molecular distances (see Table). However, proper control experiments are needed to establish the dynamic...These experiments include producing a positive control construct where the donor and the acceptor fluorophore... each other on the same protein and a negative control experiment conducted on donor-only labeled protein...protein, permeabilized cells  Cy5NTAc 32 25-45 GFP (fluorescein is similar)   Lumio Red Tc: CCPGCC 48...

  11. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral therapies such that the virus ...activated HIV-1, measured via HIV-1 promoter-driven GFP expression. In cell lines that can produce HIV-1 ...infection period rather than the ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR...

  12. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...contrast, the widely used green fluorescent protein (GFP) gives a bright green fluorescence when exposed to...genome, it is not expressed as it is under the control of a rhamnose-inducible promoter. The resolution...eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple...

  13. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...destination vector contains GFP, enabling you to select cells with high GFP expression. These cells have...cannot be released, adding temporal and/or spatial control to the system. pSQT1313 from the Joung lab allows...

  14. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...and labeled with primary antibody (0.01 µg/mL Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA...channelrhodopsin-2 or fluorescent reporter dTomato under the control of one of seven enhancers specific for different...expression of dTomato reporter (magenta) under the control of the BiPVe3 enhancer and parvalbumin (PV) immunostaining...Addgene cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene...excited to share our very own set of empty, HA-, and EGFP-expressing lentiviral backbones designed right here... packaging and strong expression after inserting EGFP and provide step-by-step protocols for viral generation...Figure 2). Looking for more? Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set...

  15. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ... laboratories to carry our independent quality control for a range of testing, from animal doping to forensics...Mammalian Cells Learn about the Dangers of Using GFP for Protein Localization Resources on Addgene.org...

  16. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...Structural Biology. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...Lindsey M. Costantini, and Erik L. Snapp. "Superfolder GFP is fluorescent in oxidizing environments when targeted...

  17. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...modifications in the same strain. MazF expression is under control of the AOX1 promoter whose expression is induced...used this cassette to knock out genes, knock in a GFP cassette, and introduced site-directed mutations,...

  18. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...SYFP2 Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-...world and corrective approaches and appropriate controls are required to make this method useful for dynamic...

  19. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...overcome by using a Tet-inducible virus to allow for controlled expression of reprogramming factors. Check out...the presence of transgenes is still an important control. Additionally, Sendai virus doesn’t rely on promoters...that multiple transfection makes it difficult to control the dose of plasmid the cells receive over the ...cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution...Ohtaka, and Mahito Nakanishi. "Novel Strategy to Control Transgene Expression Mediated by a Sendai Virus-Based...

  20. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...experiment, consider co-transfecting with GFP. This allows you to sort for GFP-positive cells and to enrich for...Engineering Using CRISPR/Cas Systems has expanded out of control. To further consolidate these FAQs, Le Cong worked...
Showing: 81 - 100 of 119 results