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We narrowed to 109 results for: crispr library

Showing: 81 - 100 of 109 results

  1. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...of the special cases below:   Pooled Library A pooled library is a set of plasmids all built with the... Pooled libraries are normally supplied as liquid DNA with all of the plasmids in the library in a single...plasmids in a pooled library can range from a few hundred to millions. Pooled libraries must be verified ...sequences cloned into it. gRNAs are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs...help center article, "Can I deposit a pooled DNA library with Addgene?" or contact us at [email protected]...

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...other CRISPR plasmids are from the Zhang lab. In fact, the Zhang lab has shared over 40,000 CRISPR-Cas9...makes lentiCRISPR v2 great? It produces a ~10 fold higher titer virus, making it ideal for CRISPR pooled...pooled library screening experiments. Find lentiCRISPR v2.(If you’ve peeked through the rest of the list ...years - the majority of these being lentiviral or CRISPR tools. How many times have these plasmids been ...lentiGuide-Puro - This plasmid expresses the S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from...come.  Resources on the Addgene blog Browse our CRISPR posts Read our viral vector blog posts Read our...guide Resources on the Addgene website Browse our CRISPR collection Find plasmids for your research  Learn...

  3. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...about CRISPR Pooled Library Experiments Additional resources on the Addgene website Find CRISPR Plasmids...on your CRISPR background with our CRISPR Guide Find Software Tools for gRNA Design    ...updated in 2022 by Susanna Stroik.  The advent of CRISPR/Cas9 has made it easier than ever to make precise... be found in “The PAM Requirement and Expanding CRISPR Beyond SpCas9.” Single base pair edits Is the location...design programs. Find Validated gRNAs for Your Next CRISPR Experiment Cas9 expression systems Addgene carries...the various delivery systems can be found here “CRISPR 101: Mammalian Expression Systems and Delivery ...genome edit can be found in our blog post entitled “CRISPR 101: Validating Your Genome Edit”. Is Cas9 not ...

  4. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources...Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids...While much of CRISPR research has focused on genome editing, numerous discoveries have been made using...genomic region of interest targeted by a gRNA. CRISPR-mediated purification of a specific genomic region...method to purify a specific genomic region using the CRISPR system, consisting of dCas9 and targeting gRNA,...require multiple cloning steps (Fujita et al., 2013). CRISPR-mediated purification of a specific genomic region...Experimental use Since the initial report describing CRISPR-mediated purification of a specific genomic region...

  5. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...exist in the CGC library but that would be useful to our model  so that we can use CRISPR/cas9 to build ...accounts with  Addgene. We now have the incredible CRISPR/cas9 tools at our disposal to to modify the worm... crispr...

  6. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...resources: plasmid, pooled library, blog posts, protocols, and more. Selecting crispr in Guides will return...for “CRISPR” plasmids keeps more than half of these. Instead we can navigate first to the CRISPR Resources... search field front and center. Let’s type in “crispr” and see what comes up. Before even clicking the...return links including our CRISPR guide.  For a closer look at this new functionality and ways to navigate...Type” you can filter by experimental purpose (e.g. CRISPR) Beyond search What can you do if you don’t have...Resources page: The page links to guides, our CRISPR eBook, plasmid kits, and curated pages categorized ...

  7. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...Muscular Dystrophy with CRISPR Gene Editing Learn to Use CRISPR Pooled Libraries for Genome-Wide Screens...became much more realistic with the advent of CRISPR. Using CRISPR/Cas9 SAM to "shock and kill" To “shock ...HIV-1 out of the genome with CRISPR/Cas9 Before the introduction of CRISPR, HIV-1 genome editing with ...updated CRISPR Guide! Find CRISPR Plasmids for Your Research Browse gRNA Design Tools  ... to vanquish these hidden viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel...University has demonstrated two potential strategies for CRISPR-HIV therapeutics - one using dCas9-SAM to activate...genome from infected cells. Read on to learn how CRISPR can take on HIV-1 in vitro, and what obstacles ...

  8. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...With the meteoric rise of CRISPR technology, the ability to direct enzymes – from nucleases to transcription...version,” she said. “If we learn how to make the library even better, we would be very happy to share updated...

  9. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...And when CRISPR arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs....org/guides/crispr/” — SirHamhands, responding to a request for “any suggestion for CRISPR online course...NIH BRAIN Initiative. We also created a growing library of antibody educational resources, including our...

  10. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...COVID-19: CRISPR-Based Detection Tools. The CRISPR Journal 3:142–145. https://doi.org/10.1089/crispr.2020.0030... Recently, scientists used the genome-wide CRISPR libraries, Brunello (knock out) and Calabrese (activation...post that highlights five different methods to use CRISPR to detect SARS-CoV-2. Other protocols and tools...Konermann S, Hsu PD (2021) Genome-wide, bidirectional CRISPR screens identify mucins as critical host factors...

  11. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...Addgene.org Find all CRISPR plasmids and resources at Addgene Find CRISPR base editors Find CRISPR plasmids for...base. If you heard about CRISPR, one question probably comes to mind: can CRISPR be applied to fix the defective..., Y. Multiplex CRISPR-mediated base editing in rice. In Genome Engineering: The CRISPR-Cas Revolution,...Addgene blog Read all of our CRISPR 101 blog posts Download the CRISPR 101 eBook Read our blog posts ... to facilitate repair. Since the development of CRISPR-mediated base editing (BE), these types of repairs...et al., 2016 and Nishida et al., 2016) invented CRISPR-base editors by tethering cytosine deaminase with...adenine (A) to a guanine (G)? You may wonder if a CRISPR-base editor can perform this change. However, there...

  12. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...using Addgene’s lentiviral CRISPR tools. Schematic overview of CRISPR/Cas9 gene editing process...described in Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F...I was expecting when I set out to make my first CRISPR/Cas9 gene edit. I wanted to inactivate the gene...objective, it was surprisingly easy. It’s true, I CRISPRed. In this post, I’ll summarize the basic steps ...homologous recombination (Rago et al., 2007). Using CRISPR, I was able to knockout BRAF in a human cancer ... in Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Doench JG, Hartenian...maximize activity and minimize off-target effects of CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, ...

  13. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...Navigate a Scientific Enterprise in Flux CRISPR Kinome Libraries Available: Pooled and Individual Plasmid...

  14. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...cells By Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells ...multispecies approach to generate and screen a library of AAV9 variants (Chuapoco et al., 2023). They ...investigating NHP brains. Figure 3: Overview of library generation and screening that led to identification...

  15. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...sgRNAs Generating constructs to produce sgRNA for CRISPR experiments can be laborious, requiring multiple... and generation of sgRNA. Generation of sgRNA libraries, with maximum complexity, is achieved with synthetic... Addtional Resources on Addgene.org Browse Our CRISPR Resources Find Validated gRNAs Browse All Plasmids...

  16. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...xCas9, a CRISPR variant with PAM flexibility Resources on Addgene.org Get the basics of CRISPR in our ...our CRISPR guide Find all plasmids for base editing in our collection Sign up to get our free CRISPR eBook... et al., 2017) in combination with a synthetic library of TadA sequences to create the eighth generation...

  17. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ... within the keratinocytes. “We hope to use CRISPR libraries from Addgene to set up assays to see what ... contraction defects. The lab also plans to use CRISPR plasmids to generate deletions of candidate genes...

  18. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ... fragments can then be purified for subsequent library preparation and high-throughput sequencing. Improvements...on Addgene.org Find simple lab protocols Browse CRISPR plasmids for isolating a genomic locus Browse FRET...

  19. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...Nature Methods. 2015 Aug 31. CRISPR-Display: A novel CRISPR technology for targeting RNA cargos...technique, called CRISPR-Display, that may help answer this difficult question. CRISPR-Display uses the...system provides users greater temporal control over CRISPR/Cas9 mediated genome modification and gene expression...139-42. doi: 10.1038/nbt.3149. Light-inducible CRISPR-Cas9 system for endogenous gene activation Optogenetics...Nucleases, the Gersbach lab has modified the RNA-guided CRISPR-Cas9 system to create a tool that is quick, versatile...robust. Dubbed the LACE system for light-activated CRISPR-Cas9 effector, Polstein and Gersbach fused the ...targeted to a very specific site on the genome via the CRISPR system. The ease and flexibility of the LACE technology...

  20. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...blog CRISPR 101: Cytosine and Adenine Base Editors Read about four base editor reporters CRISPR 101: ...Editing CRISPR 101: Cas9 Structural Biology Additional resources on Addgene.org Browse CRISPR plasmids...editing Get a broad overview with our CRISPR Guide Check out our CRISPR 101 ebook ...G-to-G•C base editors developed using CRISPRi screens, target-library analysis, and machine learning. Nature...The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base...base editing tools First, let’s briefly review how CRISPR-derived base editing works in general. Base editors... on the outside of the enzyme. Figure 2: CRISPR R-loop. Created with BioRender.com. Base editors...
Showing: 81 - 100 of 109 results