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Showing: 81 - 100 of 109 results

  1. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity...

  3. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...cutting-edge imaging technologies such as array tomography, super-resolution STORM imaging, and electron...

  6. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...to Tony from legal, Diana in finance, and Wu in graphic design. You could dread these interactions, but...

  7. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation...

  8. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...by one-step immobilized metal ion adsorption chromatography (IMAC) using Ni-NTA magnetic beads. Workflow...

  9. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...vector particles.     Figure 3: Electron micrograph of AAV vector preparation shows that the vast...

  10. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...(2024). Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders...

  12. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...: While the plot generated often looks like the graph on the left, it might make more sense once you realize...

  14. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...pitch that requires reading two pages or even a paragraph to get to the punch line is a terrible sales pitch...

  16. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...labeling it. The label can then be visualized by autoradiography and X-ray film for radioactively labeled probes...

  18. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...for mismatches within the gRNA sequence. Graphic courtesy of Maya Kostman. The most commonly...
Showing: 81 - 100 of 109 results