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We narrowed to 125 results for: grna design

Showing: 81 - 100 of 125 results

  1. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...human cells (Kim et al, 2016). The Waldman lab has designed their AAV tagging vectors (pAAV-SEPT-Acceptor ...vectors for your specific gene, the lab recommends designing homology arms with the modification of interest...use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in second a second...small Cas9 homologs like SaCas9 and their requisite gRNAs take up essentially all of an AAV’s ~4.5 kB packaging...

  2. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...replacing it or disrupting it with an piece of DNA designed in the lab. Specially constructed plasmids can...interest is difficult to target specifically with a gRNA). Be sure to keep these techniques in mind when ...locus with homologous sequence using a specially designed vector that contains sequence homology to the ... the process, we’ll walk through an experiment designed to knock out exon 2 of a given gene.   ... Figure 1: A knockout targeting vector designed to insert a resistance gene. The vector contains a neomycin... to select against random recombinants.   Design your targeting construct. For recombination to ... Figure 2: A knockout targeting vector designed to insert and later remove a resistance gene. ...

  3. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed gRNA systems...cloning, or MoClo, is a systematic cloning strategy designed by synthetic biologists to perform large scale...

  4. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...subsequently tested the original BE3 and HF-BE3 using a gRNA with high off-target cutting, and they found that...codons) with kanamycin cassettes and were carefully designed to allow for easy removal of the resistance cassette... constructs (wild-type and truncation mutants), gRNAs used for TZAP gene editing, and a Hi6xs-MBP tagged...as Cys4, tRNA, and ribozyme systems for multiplex gRNA expression. Expression of toolkit components can...compatible backbones that can be combined with your gRNA and repair templates of choice though modular cloning...frequently and has developed a website to aid in plasmid design and construction. We look forward to the many plant... a single, unique, and constitutively expressed sgRNA targeting the 5’ end of each essential gene. Koo...

  6. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...heterodimeric fusion of wild-type TadA and TadA7.10, they designed one set of ABE8’s similarly (ABE8.x-d). In order...cellular DNA and RNA dependent or independent of the gRNA used (Gaudelli et al., 2017). The authors tested...al., 2020) was able to reduce off-target RNA and gRNA-dependent DNA editing while maintaining on-target...

  7. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...the mutated spike protein and a barcode, and is designed so that the SARS-CoV-2 spike RBD is displayed ... system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool...cerevisiae genes with 6-12 guides per gene where gRNA expression is induced by anhydrous tetracycline....

  8. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...target DNA sequence as directed by the guide RNA (gRNA), while the CRY2PHR-activator fusion floats freely...shown in the figure below. Their most successful design utilized Magnet photoswitchable proteins derived...occurs. Bubeck et al. expressed a telomere-targeting gRNA and a dCas9 fusion protein with three red fluorescent...Photocleavable ssDNA oligonucleotides Cas9, photocaged-gRNA Gene editing Manna et al., 2019   NPPOC-caged ..., 2020   NPOM-caged nucleobases Cas9, photocaged-gRNA Gene editing For more details, check out this...

  10. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest. Promoter The promoter...this post, you’ve got the knowledge to be able to design a construct for the successful and specific expression...

  11. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...Origin of Replication What is a Plasmid? How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's...Electrophoresis Bacterial Transformation How to Design a Primer Creating Bacterial Glycerol Stocks Inoculating...Analysis Videos What is a Plasmid? How to Design Primers for PCR Agarose Gel Electrophoresis Inoculating...all 101s on a particular topic into beautifully designed and easy-to-reference eBooks. Figure 3: Global...changes, like using alt-text, captions, and inclusive design in our digital content. We have implemented proactive...

  12. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design Tools ...currently undergoing a renaissance. Yosef et al. designed a two-phage CRISPR system to resensitize antibiotic-resistant...Ahmed A. Gomaa, and Rodolphe Barrangou. “A CRISPR design for next-generation antimicrobials.” Genome Biol...

  13. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ... Both Snapgene and NEB have tools that help you design primers for PCR amplification of fragments to incorporate...demonstration of how to use Snapgene’s program to design primers for Gibson Assembly. For a simple example...without scars as depicted below. First, you need to design primers to amplify the two fragments while also...assembly, long primers for each fragment must be designed and ordered, and are specific for each fragment...linearized vector, the Cas9 enzyme along with a specific gRNA was used to cleave the 22kb vector. When followed...ratios of product to insert, and tools for primer design, so it’s always best to check in with the instructions...

  15. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...please improve your plasmid maps!" After thoughtful design, vetting, and tweaking, we’re excited to announce...commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display, you...

  16. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by the Loren Looger and Kaspar Podgorski...Aggarwal et al., 2025).In this latest work, the team designed and screened a large library of iGluSnFR3 mutants... HA-, and EGFP-expressing lentiviral backbones designed right here at Addgene. Here's what makes these... stable cell line creation a breeze. Modular by design: Key elements (promoter, tag, polyA site) are flanked... plasmids for flexibility and speed, so you can design, test, and innovate faster than ever. Plus, we'...scarless insertion of the payload (teal). The Cas9 gRNA targeting guide and the transposon RNA homology ...

  17. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...introduced through non-homologous end joining (NHEJ). Designing an RNA editor The Zhang lab envisioned a two-component...edit to be made when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine...various spacer lengths from 30-84 nucleotides, and designated this system REPAIRv1. Using next-generation sequencing...

  18. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... This vastly increases the possibilities when designing screens. Each serotype has its own tropism, or...dedicated to expressing Cas9, while the other houses the gRNA and scaffold. If you don’t feel like doubling the...Adames, A. C., Saur, D., & Rad, R. (2022). Tutorial: design and execution of CRISPR in vivo screens. Nature...

  20. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ... systems, you will have to design your crRNAs using other web-based design tools (for C-terminus tagging...knock-in. Another good design tool can be found at Desktop Genetics .For ssODN design, remember to incorporate...mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA. J Biotechnol...bioinformatics tool for guide RNA and donor DNA design, and implemented our optimised protocols into a...genome browser: https://www.ensembl.org/index.html Design crRNA/ssODN: Using Ensembl Transcript ID(s) as ...Dewari et al. 2018). Using the first crRNA we designed for ~250 genes, we have had about 30% tagging ... in a couple of weeks by combining our “TAG-IN” design tool with the csRNP method. We anticipate that ...
Showing: 81 - 100 of 125 results