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We narrowed to 256 results for: human dat

Showing: 81 - 100 of 256 results

  1. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...becoming available. At the same time advances in human genetics led to the identification of several disease-causing...visual impairment that faithfully recapitulated the human condition. Luck brought all these key elements together...therapy. It is not known to cause any disease in humans, and its low immunogenicity is critical when systemic...results in preclinical studies some potential candidates for rare to ultra-rare ocular diseases are being... co-founded a non-profit to help push forward candidate therapies for these diseases. Riding on the early...

  2. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ... Editor of Naturejobs, part of Nature magazine. Humans are built to enjoy, remember, and listen to narratives... asking someone how and why they left academia. Humans are designed to pay attention and enjoy anecdotal...around the self, too — when talking to friends or dates or colleagues we tell tales about parts of our lives...email address far and wide, keep your website up to date, invite people to ask questions, be approachable...

  3. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...proteins is essential for human health. Failure to do so is implicated in several human diseases. Proper patterning..., etc., again in clear terms. "Implications for human disease" is vague. Instead, consider "A mechanistic... your candidacy. A major reason that a lot of candidates either get no interviews, or get several interviews...accomplish. No matter how many times I say this, candidates regularly try to convince me that their search...Nothing turns off an interviewer faster than when a candidate says "I'm not an expert in [your research area...with feedback from their own job searches. I've updated chapters to reflect new technologies, i.e. video...I'll try to answer your question and subsequently update the ebook. One of my graduate students encouraged...

  4. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...description of the VGS process and use cases in Human Gene Therapy. Existing methods for AAV quality control...Adeno-Associated Viral Genomes in Academic Settings. Human Gene Therapy Methods 30:195–205 . https://doi.org/10.1089/...the sequencing data from multiple AAV lots can be run in parallel and provide serotype data in minutes and...service in 2016, we adapted this platform to accommodate adeno-associated viral vector (AAV) samples and...open-source custom Python script that uses the VGS data to determine the percent of recombination present...pre-defined serotype specific signature sequences in the data. Unlike previously described thermodynamics-based...Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of Packaged Single-Stranded Adeno-Associated ...

  5. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ... use in revealing antigenic differences between human, canine and simian isolates. J Gen Virol., 68 (Pt...Mycobacterium abscessus, an increasingly common human pathogen notorious for its high resistance to antibiotics...luciferase is embedded in the I10 domain of the human titin protein and only exposed when the module unfolds...from Addgene!   Figure 1: Experimental validation for Anti-V5 [IPI-SV5-Pk1] in a western blot assay...design and studies of protein ubiquitination and degradation.   Find the CRBNmidi plasmid here!   Kroupova...

  6. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ...CRISPR knockout screens, the Bassik lab created a human knockout library that has 10 gRNAs per gene and ...on-target activity in high-throughput screens. The human and mouse versions of the Bassik CRISPR Knockout...genomic perturbation screens: gold standards for human functional genomics." Molecular systems biology ...infected with Cas9. Validating the Library The CRISPR knockout library was validated via a growth screen...

  7. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...economic and cultural importance of these animals to human populations around the world. Here at Addgene, we...revisit some of the ways these animals have impacted humans through biomedical research. The Camelid family...radioactively labeled sdAbs have become appealing candidates for the detection of different cancers (Jin, ...

  8. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...have emerged as a powerful model for understanding human physiology, development, and disease. In order to... to morphogenesis, cell fate specification, and human disease.     Sun, et al. bioRxiv. 2021. https:/...plants, this approach was also used to build and validate a ratiometric fluorescent biosensor based on the...

  9. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...neurotransmission. But with approximately 950 different human genes encoding GPCRs (Takeda S et al., 2002) and...Identification of G protein-coupled receptor genes from the human genome sequence. FEBS Letters 520:97–101 . https...donor and a fluorescent acceptor. TRUPATH uses an updated version of BRET called BRET2. Figure 1: TRUPATH...

  10. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...my target sequence into a backbone that uses the human U6 promoter to drive expression, is it necessary...nucleotide to the start of my target sequence? A2: The human U6 promoter prefers a 'G' at the transcription start...Systems has expanded out of control. To further consolidate these FAQs, Le Cong worked with Addgene Senior...

  11. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...health-related single nucleotide polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides a method to detect nucleic...targeted to a specific DNA sequence, such as the Human papilloma virus (HPV) genome, via a crRNA. An ssDNA-fluorescently...two similar types of HPV, HPV16 and HPV18, from human cells, at atomolar levels, within one hour. DETECTR...but is undergoing validation studies.     Jennifer Tsang contributed to updating this article. References...Originally published Aug 30, 2018 and updated April 16, 2020. Sensitive and specific nucleic acid detection...Doudna lab has used Cas12a’s non-specific ssDNA degradation to develop the method termed DNA Endonuclease...DETECTR harness the promiscuous cleavage and degradation of neighboring ssRNA and ssDNA by Cas13 and Cas12a...

  12. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ... recent plasmid deposit please sign up here.  Human whole genome sgRNA iBAR library Article contributed...Since the sgRNAiBAR library covers every annotated human gene, it enables genome-wide screening with a high...one gRNA. The iBAR method resulted in high quality data even when used at a high multiplicity of infection...

  13. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...our recent blog post. Fatima Enam: Biosensors for human milk oligosaccharides Fatima Enam is currently a...developing fluorescent protein-based biosensors for Human Milk Oligosaccharides (HMOs). HMOs are thought to...development as a researcher and science communicator.  Update (7/17/2018): Check out Fatima's recent publication...

  14. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...Lin, Steven, et al. "Enhanced homology-directed human genome engineering by controlled timing of CRISPR... "Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins...nucleofection as well as the sequences of our validated editing reagents can be found on our plasmid pages...

  15. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...-editing field. It has the possibility to treat human genetic diseases, and several treatments are currently.... “Advances in CRISPR/Cas-based Gene Therapy in Human Genetic Diseases.” Theranostics. https://doi.org...originally written in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an Addgene co-op....” Cystic fibrosis may not be the most likely candidate for gene therapy, however, because the disease...

  16. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ... Kroemer often functions as one of the “healthy human volunteers” in his studies! This was followed by...He also highlighted the UCSF Immunoprofiler - a human biopsy analysis platform focused on analysing immune... guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University! Cancer therapeutics ... potential to become an open-source pipeline and data repository for cancer researchers. Go open-source...

  17. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...novel form of AI that can learn new tasks and beat humans at the ancient Chinese game Go). In addition, there...disciplines to better understand neurobiology and human cognition. Developing better AI programs gives neurobiologists...really in all of biology) require large data sets. Whether these data sets include videos of behavior, many...

  18. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    .... et al. Isolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Meth... mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature Biotechnology 31, 822–826 (2013). ...genome editing. When it comes time to (hopefully) validate successful mutation of your favourite gene, there...guide you through the rough waters of mutation validation, such as this "CRISPR 101" post. Next-generation...While these methods paved the way for mutation validation, we found these assays frustrating to work with...post, we’ll introduce digital PCR as an emerging validation technology. Digital PCR has several advantages...editing workflow. Digital PCR comparison to other validation methods As mentioned, these assays have several...

  19. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...lentiviral prep. It's a human CRISPR inhibition library that inhibits over 18,000 human genes and is used for...quality control. For an estimate on availability date or to be notified when a particular catalog item...

  20. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...targeting the entire genome, overexpression of all human transcription factors, barcoded libraries to trace...features make them an ideal delivery system for human patients. Multiple viral-based gene therapies have...emerging viral applications and give you some recommendations for putting viruses to work. Viral applications...which target complementary mRNA sequence for degradation. Viral introduction of shRNA plasmids can induce...
Showing: 81 - 100 of 256 results