We narrowed to 141 results for: ipp

1. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...lentiviral in nature, so please ensure that you are equipped and authorized to make and use lentivirus. Also...

2. dTAG - You're it!

   Type

   Blog Post

   Published

   June 21, 2018, 2:06 p.m.

   ...co-opt E3 ligases such as cereblon (CRBN) or von Hippel-Lindau (VHL) to bring the endogenous degradation...

3. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   Published

   April 10, 2018, 12:56 p.m.

   ...stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies are, however, compatible with most...

4. Visualizing Protein Turnover In Situ

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   Blog Post

   Published

   Jan. 16, 2018, 3:20 p.m.

   ...dynamics of newly synthesized proteins in rat hippocampal neurons." Nature neuroscience 13.7 (2010): 897...

5. Addgene: The First Twenty Years

   Type

   Blog Post

   Published

   Dec. 19, 2024, 2:15 p.m.

   ... our repository grew to over 150,000 plasmids, shipped out our two millionth item, and had a cumulative...

6. Antibodies 101: Selecting the Right Antibody

   Type

   Blog Post

   Published

   March 15, 2022, 1:15 p.m.

   ...feel free to make the decision by price point, shipping time, or other factors. Just remember that these...

7. Transferable Skills Guide: Problem Solving

   Type

   Blog Post

   Published

   Nov. 30, 2021, 2:15 p.m.

   ...help convince a future employer that you are well equipped to work through challenges from day one in your...

8. Human Germline Editing Using CRISPR

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   Blog Post

   Published

   Aug. 10, 2017, 2:19 p.m.

   ...always come with some uncertainty. As biologists equipped to understand the results of this paper, we should...

9. Plasmids 101: The protein expression toolbox

   Type

   Blog Post

   Published

   Feb. 16, 2023, 7:05 p.m.

   ...domains (DD) are akin to degron tags, just with a flipped mechanism. When a protein is tagged with a DD, ...

10. RNA Extraction Without A Kit

    Type

    Blog Post

    Published

    March 24, 2020, 1:15 p.m.

    ... mid 1980’s set out to develop a protocol that skipped the ultracentrifugation altogether. Chomczynski...

11. Developing Lab Management Software for Biology

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    Blog Post

    Published

    Aug. 25, 2016, 2:30 p.m.

    ...technicians to retrieve the sample and get it ready for shipping. In the first case, we naively assume that everything...

12. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    Published

    Sept. 22, 2015, 2:30 p.m.

    ...work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by one,” and she is...

13. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...personalized medicine (2017).   1st Gen AdVs are stripped of regulatory genes E1 and E3. Without these genes...

14. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ...to divide. However, if fueled with caffeine and equipped with the pDCAF3 operon, the growth of this knockout...

15. Lab Automation at Addgene

    Type

    Blog Post

    Published

    March 22, 2018, 1:28 p.m.

    ...vial fiIling The majority of Addgene plasmids are shipped as agar stabs in glass vials. Have you ever wondered...

16. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ...-1711-4 Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson...

17. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...accessible genome editing tool.   References Mangeot, Philippe E., et al. "Genome editing in primary cells and...

18. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ... tracking the synaptic maturation pattern of hippocampal adult-born granule cells over time that were ...

19. Corralling and Cornering the Goldilocks Antibody

    Type

    Blog Post

    Published

    Aug. 9, 2022, 1:15 p.m.

    ... as showing a very bright signal only in the hippocampus compared to other regions, that informs us that...

20. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...involved in education advocacy non-profits like Phipps Conservatory or the Brilliant Club and share your...