We narrowed to 141 results for: ipp

1. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...lentiviral in nature, so please ensure that you are equipped and authorized to make and use lentivirus. Also...

2. dTAG - You're it!

   Type

   Blog Post

   Published

   June 21, 2018, 2:06 p.m.

   ...co-opt E3 ligases such as cereblon (CRBN) or von Hippel-Lindau (VHL) to bring the endogenous degradation...

3. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   Published

   April 10, 2018, 12:56 p.m.

   ...stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies are, however, compatible with most...

4. Visualizing Protein Turnover In Situ

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   Blog Post

   Published

   Jan. 16, 2018, 3:20 p.m.

   ...dynamics of newly synthesized proteins in rat hippocampal neurons." Nature neuroscience 13.7 (2010): 897...

5. RNA Extraction Without A Kit

   Type

   Blog Post

   Published

   March 24, 2020, 1:15 p.m.

   ... mid 1980’s set out to develop a protocol that skipped the ultracentrifugation altogether. Chomczynski...

6. Human Germline Editing Using CRISPR

   Type

   Blog Post

   Published

   Aug. 10, 2017, 2:19 p.m.

   ...always come with some uncertainty. As biologists equipped to understand the results of this paper, we should...

7. Antibodies 101: Selecting the Right Antibody

   Type

   Blog Post

   Published

   March 15, 2022, 1:15 p.m.

   ...feel free to make the decision by price point, shipping time, or other factors. Just remember that these...

8. Transferable Skills Guide: Problem Solving

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   Blog Post

   Published

   Nov. 30, 2021, 2:15 p.m.

   ...help convince a future employer that you are well equipped to work through challenges from day one in your...

9. Addgene: The First Twenty Years

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   Blog Post

   Published

   Dec. 19, 2024, 2:15 p.m.

   ... our repository grew to over 150,000 plasmids, shipped out our two millionth item, and had a cumulative...

10. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    Published

    Feb. 16, 2023, 7:05 p.m.

    ...domains (DD) are akin to degron tags, just with a flipped mechanism. When a protein is tagged with a DD, ...

11. Developing Lab Management Software for Biology

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    Blog Post

    Published

    Aug. 25, 2016, 2:30 p.m.

    ...technicians to retrieve the sample and get it ready for shipping. In the first case, we naively assume that everything...

12. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    Published

    Sept. 22, 2015, 2:30 p.m.

    ...work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by one,” and she is...

13. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...personalized medicine (2017).   1st Gen AdVs are stripped of regulatory genes E1 and E3. Without these genes...

14. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ...to divide. However, if fueled with caffeine and equipped with the pDCAF3 operon, the growth of this knockout...

15. Lab Automation at Addgene

    Type

    Blog Post

    Published

    March 22, 2018, 1:28 p.m.

    ...vial fiIling The majority of Addgene plasmids are shipped as agar stabs in glass vials. Have you ever wondered...

16. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ... tracking the synaptic maturation pattern of hippocampal adult-born granule cells over time that were ...

17. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...involved in education advocacy non-profits like Phipps Conservatory or the Brilliant Club and share your...

18. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...accessible genome editing tool.   References Mangeot, Philippe E., et al. "Genome editing in primary cells and...

19. Corralling and Cornering the Goldilocks Antibody

    Type

    Blog Post

    Published

    Aug. 9, 2022, 1:15 p.m.

    ... as showing a very bright signal only in the hippocampus compared to other regions, that informs us that...

20. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ...-1711-4 Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson...