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We narrowed to 121 results for: lentiviral vector

Showing: 81 - 100 of 121 results

  1. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in...developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene...N.C., Goeden, N. et al. Adeno-associated viral vectors for functional intravenous gene transfer throughout...region VIII of AAV9-X1.1 to that of AAV1. This viral vector has brain endothelial cell-specific tropism, with... by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing. In a ...

  4. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ... as RNAP II promoters is commonly employed in lentiviral and retroviral constructs and we will discuss...discuss these in a future post on viral vector parts. Promoter specificity Aside from choosing a promoter based... Can be induced by IPTG. Commonly found in pET vectors. Very tightly regulated by the lac operators. Good...-inducer fucose Weaker. Commonly found in pBAD vectors. Good for rapid regulation and low basal expression...Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP...

  5. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...Viral Vectors 101: An Introduction to AAV Viral Vectors 101: AAV Variables that Matter Viral Vectors 101...choice of AAV vector for your experimental system. AAV1 AAV1 was the first viral vector approved for gene...by Adenovirus, Adeno-Associated Virus, and Lentiviral Vectors After Intravenous and Direct Kidney Injections...A viral vector that can target specific tissues, even when administered systemically, without causing...causing disease? Recombinant adeno-associated viral vectors, or rAAVs, can sound almost too good to be true...components. Created with BioRender.com. AAV vectors have both distinctive capsids and genomic structures...specific cells. This protein shell packages the vector’s genetic material, and variations between serotypes...

  7. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...differentiation Check out our lentivirus guide Sign up to receive our Viral Vectors 101 eBook ...single-cell level with CellTag CellTagging is a lentiviral based tagging method that labels cells with heritable...

  8. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some...performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic overview of CRISPR...gift from Feng Zhang and is described in Improved vectors and genome-wide libraries for CRISPR screening....Lines with Lentivirus Isolating a Monoclonal Cell Population by Limiting Dilution Lentivirus Production...of A549 cells transduced with Cas9 expressing lentivirus at the indicated multiplicity of infection (MOI...

  9. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ... a) Cells are transfected with three different vectors and flow cytometry is used to sort populations ... 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker...

  10. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...biosensor in optimal proportions from a vector backbone that allows lentiviral packaging as a single payload (...recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors (Janicot ...

  13. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ....org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources Request ready-to-use...-to-use viral preps of select CRISPR lentiviral plasmids Additional resources on the Addgene Blog  How...

  14. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...kit has a focus on infrastructure with shuttle vectors, several delivery systems which can be engineered...engineered into the TUs, and even viral gene silencing vectors. The Plant Parts III kit has a focus on transformation...Fragmid system was used to assess how different lentiviral plasmid backbone elements impact viral titer ...Gantner, Johannes, et al. “Peripheral Infrastructure Vectors and an Extended Set of Plant Parts for the Modular....0197185. Chamness, James C., et al. “An Extensible Vector Toolkit and Parts Library for Advanced Engineering...Rapid Design and Production of Diverse Expression Vectors.” ACS Synthetic Biology, vol. 6, no. 7, American...acssynbio.7b00016. McGee, Abby V., et al. Modular Vector Assembly Enables Rapid Assessment of Emerging CRISPR...

  15. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...Differentiate the Stem Cell Field Learn how lentiviral vectors can be used for gene delivery Resources ...and in vitro methods are similar except that the vectors carrying the transdifferentiation factors are directly... (iPSCs) by temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci U S A, 2011. 108(34): p. 14234...., Human induced pluripotent stem cells free of vector and transgene sequences. Science, 2009. 324(5928...mouse induced pluripotent stem cells without viral vectors. Science, 2008. 322(5903): p. 949-53. PubMed PMID...Addgene.org Visit our Stem Cell Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka...common delivery methods include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid...

  16. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...processing Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA...most often expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs...cloning strategies for constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org/10.1186...

  17. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...millions of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same family...family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified...cells. Remove this pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing...

  18. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools Viral Vectors 101:...create far safer viral vectors that can be used in BSL-1 or BSL-2 labs. Viral vectors are viruses that have...increase the viral vector’s carrying capacity. In order to allow the viral vectors to reproduce when experimentally...expressed via cells cultured with the vectors. This allows the viral vectors to reproduce only in experimentally...of Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral Vectors 101 eBook...: AAVs, lentivirus, gamma retrovirus, and adenovirus. And now that you know what viral vectors are, it...viral particle is called a virion. Finally, viral vectors, which are used in the lab, are distinct from viruses...

  19. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ... used - GUIDE-Seq and integrase-defective lentiviral vectors - which rely on insertion of DNA by the NHEJ...

  20. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...SspB-mCherry-p60 katanin module, which are also available as lentiviral plasmids if you want to make a stable cell line...Additional resources on the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org...
Showing: 81 - 100 of 121 results