We narrowed to 122 results for: lentiviral vector

...facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in...developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene...N.C., Goeden, N. et al. Adeno-associated viral vectors for functional intravenous gene transfer throughout...region VIII of AAV9-X1.1 to that of AAV1. This viral vector has brain endothelial cell-specific tropism, with... by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing. In a ...

...protein in macrophages/monocytes using lentiviral expression vectors. I also used plasmids made and shared...

...high-throughput manner. They designed the pXPR_053 lentiviral expression vector, which contains an sgRNA insert site.... 2018. PubMed PMID: 29300010. miRNA reporter vectors shed new light on mechanisms of miRNA-mediated ...

... as RNAP II promoters is commonly employed in lentiviral and retroviral constructs and we will discuss...discuss these in a future post on viral vector parts. Promoter specificity Aside from choosing a promoter based... Can be induced by IPTG. Commonly found in pET vectors. Very tightly regulated by the lac operators. Good...-inducer fucose Weaker. Commonly found in pBAD vectors. Good for rapid regulation and low basal expression...Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP...

...via a lentiviral vector to cell lines that either stably expressed Cas9 or that had been lentiviral infected...cheat sheet Read our Quick Guide to All Things Lentivirus Resources on Addgene.org Check out this guide...

...In addition, several libraries are offered as lentiviral preparations to save you a few steps! To check...Additional resources on the Addgene blog Viral Vectors 101: Preparing Pooled Libraries Pooled Library ...

...differentiation Check out our lentivirus guide Sign up to receive our Viral Vectors 101 eBook ...single-cell level with CellTag CellTagging is a lentiviral based tagging method that labels cells with heritable...

...expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some...performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic overview of CRISPR...gift from Feng Zhang and is described in Improved vectors and genome-wide libraries for CRISPR screening....Lines with Lentivirus Isolating a Monoclonal Cell Population by Limiting Dilution Lentivirus Production...of A549 cells transduced with Cas9 expressing lentivirus at the indicated multiplicity of infection (MOI...

... a) Cells are transfected with three different vectors and flow cytometry is used to sort populations ... 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker...

...biosensor in optimal proportions from a vector backbone that allows lentiviral packaging as a single payload (...recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors (Janicot ...

... study of SARS-CoV-2 are the lentiviral production and packaging vectors VSV-G and psPAX2, which were ...

....org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources Request ready-to-use...-to-use viral preps of select CRISPR lentiviral plasmids Additional resources on the Addgene Blog  How...

...women with TS (Trolle et al., 2016). Using lentiviral vectors from Addgene, the lab will express these ...

...kit has a focus on infrastructure with shuttle vectors, several delivery systems which can be engineered...engineered into the TUs, and even viral gene silencing vectors. The Plant Parts III kit has a focus on transformation...Fragmid system was used to assess how different lentiviral plasmid backbone elements impact viral titer ...Gantner, Johannes, et al. “Peripheral Infrastructure Vectors and an Extended Set of Plant Parts for the Modular....0197185. Chamness, James C., et al. “An Extensible Vector Toolkit and Parts Library for Advanced Engineering...Rapid Design and Production of Diverse Expression Vectors.” ACS Synthetic Biology, vol. 6, no. 7, American...acssynbio.7b00016. McGee, Abby V., et al. Modular Vector Assembly Enables Rapid Assessment of Emerging CRISPR...

...Differentiate the Stem Cell Field Learn how lentiviral vectors can be used for gene delivery Resources ...and in vitro methods are similar except that the vectors carrying the transdifferentiation factors are directly... (iPSCs) by temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci U S A, 2011. 108(34): p. 14234...., Human induced pluripotent stem cells free of vector and transgene sequences. Science, 2009. 324(5928...mouse induced pluripotent stem cells without viral vectors. Science, 2008. 322(5903): p. 949-53. PubMed PMID...Addgene.org Visit our Stem Cell Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka...common delivery methods include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid...

...processing Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA...most often expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs...cloning strategies for constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org/10.1186...

...millions of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same family...family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified...cells. Remove this pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing...

...true" %}{% raw %}"Browse Empty gRNA Expression Vectors"{% endraw %}{% end_module_attribute %}{% module_attribute...href":"https://www.addgene.org/crispr/empty-grna-vectors/","type":"EXTERNAL"},"open_in_new_tab":true,"no_follow... guide (synthetic, in vitro transcription, or lentiviral delivery) can affect the accuracy of a predictive...

... used - GUIDE-Seq and integrase-defective lentiviral vectors - which rely on insertion of DNA by the NHEJ...

...SspB-mCherry-p60 katanin module, which are also available as lentiviral plasmids if you want to make a stable cell line...Additional resources on the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org...