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...transduction of both the CNS and PNS.That is until the Gradinaru Lab at Caltech stepped up to the challenge. Find...therapy treatments for neurological diseases). The Gradinaru Lab has a lot going on. Here are the key takeaways...

...makers have moved away from long written proposal narratives. Now they require grant seekers to use online...demand for stronger budgets with clear budget narratives, logic models, established partnerships and collaborations...

...mice is likely due to how the Deverman and the Gradinaru labs engineered PHP.B. PHP.B was created by first...phenotype. Exome sequencing of related hybrid mice narrowed the search to a ~4.5 Mbp region of the mouse chromosome...

...replicate in, and persist in. Some plasmids have a narrow host range, while others have a broad host range...2009). Figure 1: Idealized examples of a narrow host range plasmid with only one ori and a broad...

...to see these populations, I suggest using the imaginary quadrant to help (Figure 5). Even though this ...is define a point where the peak is sufficiently narrow to count as a “true” signal.  Figure 8: ...

...CBE. Base editors for the plant genome These preliminary studies demonstrated successful base editing ...than one genomic site simultaneously.     These binary vectors could be used to generate stable genome...

...some microscopy enthusiasts to start a microscopy seminar to connect the imaging community and to create ...for lunch, make a point of talking to them at a seminars, or even set up regular meetings. Meeting people...

... courses of treatment with antibiotics These scenarios expose bacterial populations to antibiotics at...counter-intuitive considering the other three scenarios, but a short treatment regiment might wipe out...

...introductory teaching and research environments (Idalia & Bernardo, 2017).  Escherichia coli. Image from NIAID...doi.org/10.1242/dmm.002733 Idalia, V.-M. N., & Bernardo, F. (2017). Escherichia coli as a Model Organism...

...BioRender.com   The first couple of steps are preliminary steps that are not exclusive to IF, but they ... epitope, and sample type (Im, et al. 2019). To narrow down which specific methods to use, start by reading...

...with early career science policy groups, attend webinars, and learn more about the field.  Read the CCST...accelerator for some hands-on guidance.   Check out my webinar via the NC Science Policy student group SciPolPack...

...fellowship grants) A project with sufficient preliminary data to establish your own lab and get an independent...their research. They dread giving a departmental seminar or a joint lab meeting. I've been told that PFs...

...purchased from various other suppliers with a typical turnaround time of 5-7 days. The crRNA and tracrRNA are ...from commercial suppliers of CRISPR reagents (turnaround time 5-7 days); because the tracrRNA is the same...

...during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN’s QIAwave, and Milllipore Sigma’s GenElute...in plasmid purification kits Kit Series NEB Monarch QIAGEN QIAwave Millipore Sigma GenElute Reduced...

... studies with clinical endpoints. In the first scenario, robust and efficient generation of iPSCs is the...better suited for these situations. The second scenario requires higher levels of safety and as little...

...onto other proteins to confer functionality. In Narayan et al., they used this now classic technique to... of their useful reagents shared via Addgene. Narayan S, et al. Cell Reports. 2017. PubMed PMID: 28813671...

...CapsidsResearchers: Cynthia Arokiaraj; Viviana Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation...

...and Deeper” by Jorge Cham   2)   “Boxplot” by Maki Naro   3)   “Bird and Moon” by Rosemary Mosco   4)   ...

...filters on the right-hand side of the search page to narrow your keyword search to one of these organisms. ...

...other video series.   And if you haven’t seen our webinar “Navigating an Expanding Landscape for Engineered...